Studies on the control mechanism of osteoclast differentiation by non-coding RNA

• Project/Area Number: 18390497
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pathobiological dentistry/Dental radiology
• Research Institution: Tokyo Medical and Dental University
• Principal Investigator: SHIMOKAWA Hitoyata Tokyo Medical and Dental University, 大学院・医歯学総合研究科, 准教授 (80014257)
• Co-Investigator(Kenkyū-buntansha): 青木 和広 東京医科歯科大学, 大学院・医歯学総合研究科, 助教 (40272603)
• Research Collaborator: WANTIDA Sriarj 東京医科歯科大学, 大学院・医歯学総合研究科, 大学院生
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥15,360,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥1,260,000); Fiscal Year 2008: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000); Fiscal Year 2007: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2006: ¥9,900,000 (Direct Cost: ¥9,900,000)
• Keywords: 発生・分化 / 発現制御 / マイクロアレイ / 破骨細胞 / miRNA / 発現製御

Research Abstract

19年度は共存培養による破骨細胞の分化誘導系を用いて得られた十数種類のmiRNAについて個々に解析を行った。そのうちの数種類について変化が確かめられた。
20年度は骨髄細胞を用いた破骨細胞形成系により確かめることと、個々のmiRNAについての解析を行った。

Research Products

• [Journal Article] Inhibition of the classical NF-kB pathway prevents osteoclast bone-resorbing activity (2009)
  • Author(s): Soysa N, Alles N, Shimokawa H, Jimi E, Aoki K, Ohya K
  • Journal Title: J Bone Mrtab Vol.27(2) Pages: 131-139
• [Journal Article] Bovine dentine organic matrix down-regulates osteoclast activity (2009)
  • Author(s): Wantida S, Aoki K, Ohya K, Takagi Y, Shimokawa H
  • Journal Title: J Bone Miner Metab Vol.27 Pages: 315-323
  • NAID: 10025337365
• [Journal Article] Inhibition of the classical NF-kappaB pathway prevents osteoclast bone-resorbing activity. (2009)
  • Author(s): Soysa, N. S., Alles. Shimokawa, H., Jimi, E., Aoki, K., Ohya, K.
  • Journal Title: J Bone Miner Metab 27 Pages: 131-9
  • Peer Reviewed
• [Journal Article] Bovine dentine organic matrix down-regulates osteoclast activity. (2009)
  • Author(s): Sriarj, W., Aoki, K., Ohya, K., Takagi, Y., Shimokawa, H.
  • Journal Title: J Bone Miner Metab 27 Pages: 315-23
  • NAID: 10025337365
  • Peer Reviewed
• [Journal Article] Insulin-like Growth Factor I Regulates Apoptosis in Condylar Cartilage (2008)
  • Author(s): Yokota T, Shipmokawa H, Shibata S, Itoh K, Baba Y, Ohya K, Ohyama K, Suzuki S
  • Journal Title: J Dent Res Vol.87(2) Pages: 159-163
• [Journal Article] Lipopolysaccharide-induced bone resorption is increased in TNF type 2 receptor-deficient mice in vivo (2008)
  • Author(s): Mian MD, Saito H, Alles N, Shimokawa H, Aoki K, Ohya K
  • Journal Title: J Bone Miner Metab Vol.26 Pages: 469-477
  • NAID: 10024459458
• [Journal Article] Bone Morphogenetic Protein-2(BMP-2)and Vascular Endothelial Growth Factor(VEGF)Transfection to Human Periosteal Cells Enhances Osteoblast Differentiation and Bone Formation (2008)
  • Author(s): Samee M, Kasugai S, Kondo H, Ohya K, Shimokawa H, Kuroda S
  • Journal Title: J Pharmacol Sci Vol.108 Pages: 18-31
  • NAID: 10024322237
• [Journal Article] Lipopolysaccharide-induced bone resorption is increased in TNF type 2 receptor-deficient mice in vivo. (2008)
  • Author(s): Hussain Mian, A., Saito, H., Alles, N., Shimokawa, H., Aoki, K., Ohya, K.
  • Journal Title: J Bone Miner Metab 26 Pages: 469-77
  • NAID: 10024459458
  • Peer Reviewed
• [Journal Article] A tumor necrosis factor receptor loop peptide mimic inhibits bone destruction to the same extent as anti-tumor necrosis factor monoclonal antibody in murine collagen-induced arthritis. (2007)
  • Author(s): Saito H. Kojima T, Takahashi M, Horne WC, Baron R, Amagasa T, Ohya K, Aoki K.
  • Journal Title: Arthritis and rheumatism 56 Pages: 1164-1174
  • Peer Reviewed
• [Journal Article] Small GTPase Ras and Rho Expression in Rat Osteoblasts during Spaceflight. (2007)
  • Author(s): Kumei Y, Shimokawa H. Ohya K, Katano H. Akiyama H, Hirano M, Morita S.
  • Journal Title: Annals of the New York Academy of Sciences 1095 Pages: 292-299
  • Peer Reviewed
• [Presentation] The control release of TNF-α is necessary to mimic the LPS-induced bone resorption on calvaria (2009)
  • Author(s): Nagano K, Aoki K, Mian H, Alles N, Shimokawa H, Morimoto N, Akiyoshi K, Ohya K
  • Organizer: 第82回日本薬理学会年会
  • Place of Presentation: 横浜
• [Presentation] Effects of Dentine Organic Matrices on Osteoclastogenesis. (2008)
  • Author(s): Sriarj, W., Aoki, K., Ohya, K., Takagi, Y., Shimokawa, H.
  • Organizer: 86^<th> General Session & Exhibition of the IADR
  • Place of Presentation: Toronto, Canada
  • Year and Date: 2008-07-03
• [Presentation] Non-canonical NF-κB pathway regulates osteoclast formation and function (2008)
  • Author(s): Soysa N, Alles N, Aoki K, Shimokawa H, Jimi E, Ohya K
  • Organizer: The 81^<th> Annual Meeting of the Japanese Pharmacological Society(81回日本薬理学会年会)
  • Place of Presentation: 横浜
• [Presentation] NIK MUTATION IN ALY/ALY MICE CAUSES OSTEOPETROSIS AND REDUCE RANKL INDUCED OSTEOCLASTOGENESIS (2008)
  • Author(s): Soysa N, Alles N, Mian MD, Yasuda H, Jimi E, Shimokawa H, Aoki K, Ohya K
  • Organizer: 2^<nd> International Conference on Osteoimmunology : Interactions of the Immune and Skeletal Systems
  • Place of Presentation: Rhodes(Greece)
• [Presentation] Defective Alternative Pathway of NF-κB Causes Mild Osteopetrosis in Mice (2008)
  • Author(s): Soysa N, Alles N, Aoki K, Shimokawa H, Jimi E, Ohya K
  • Organizer: 86^<th> General Session & Exhibition of the IADR
  • Place of Presentation: Toronto
• [Presentation] Effects of Dentin Organic Matrices on Osteoclastogenesis (2008)
  • Author(s): Sriarj W, Aoki K, Ohya K, Takagi Y, Shimokawa H
  • Organizer: 86^<th> General Session & Exhibition of the IADR
  • Place of Presentation: Toronto
• [Presentation] LPS投与による抜歯後骨新生の抑制はTNF-α依存的である (2008)
  • Author(s): 友松伸允, 青木和広, Mian MD, 下川仁彌太, 天笠光雄, 大谷啓一
  • Organizer: 第28回日本骨形態計測学会
  • Place of Presentation: 東京
• [Presentation] LPS-induced Inhibition of Osteogenesis is TNF-α Dependent (2008)
  • Author(s): Tomomatsu N, Aoki K, Hussain MA, Alles N, Shimokawa H, Amagasa T, Ohya K
  • Organizer: 30^<th> ASBMR Annual Meeting
  • Place of Presentation: Montreal
• [Presentation] 破骨細胞機能抑制ペプチドによる臨床応用の可能性 (2008)
  • Author(s): 青木和広, Alles N, Soysa N, 自見英治郎, 下川仁彌太, 大谷啓一
  • Organizer: 第50回歯科基礎医学会学術大会・総会
  • Place of Presentation: 東京