High Performance Ti implant by photo-immobilization of cytokine and its effect oncell adhesion, proliferation and differentiation

• Project/Area Number: 18390513
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 補綴理工系歯学
• Research Institution: Okayama University
• Principal Investigator: YOSHIDA Yasuhiro Okayama University, 大学院・医歯薬学総合研究科, 准教授 (90281162)
• Co-Investigator(Kenkyū-buntansha): SUZUKI Kazuomi 岡山大学, 大学院・医歯薬学総合研究科, 教授 (30050058) ; YAMAMOTO Toshio 岡山大学, 大学院・医歯薬学総合研究科, 教授 (30107776) ; NAGAOKA Noriyuki 岡山大学, 大学院・医歯薬学総合研究科, 教授 (70304326) ; ITO Yoshihiro 理化学研究所, 伊藤ナノ医工学研究室, 研究員 (40192497)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥18,220,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥2,820,000); Fiscal Year 2008: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2007: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2006: ¥6,000,000 (Direct Cost: ¥6,000,000)
• Keywords: 歯科理工学 / 歯科材料学 / 生体材料学 / 歯科インプラント学 / サイトカイン / チタン / 光固定 / インプラント / 固定 / 光反応 / 細胞接着 / 増殖 / 分化

Research Abstract

インプラント周囲の組織再生能を向上すべく、様々な表面改質法が考案されている.しかし生体分子の固定化に関しては、未だ実用的な手法は報告されていない.本研究では、光反応性ゼラチンによるチタン表面への生体分子固定化法を新たに開発し、その結果、チタン表面にゼラチンを微細構造状に光固定化できることが明らかとなった.様々な生体分子を共固定化することにより、チタン表面への生体機能性付与が可能となると期待される.

Research Products

• [Journal Article] Polyphosphoric acid treatment promotes bone formation around titanium implants (2009)
  • Author(s): Maekawa, K., Shimono, K., Oshima, M., Yoshida, Y., Van Meerbeek, B., Suzuki, K., Kuboki, T.
  • Journal Title: Journal of Oral Rehabilitation 36 Pages: 362-367
• [Journal Article] Antibacterial effect of baresin matrix (2009)
  • Author(s): Namba, N., Yoshida, Y., Nagaoka, N., Takashima, S., Matsuura-Yoshimoto, K., Maeda, H., Van Meerbeek, B., Suzuki, K., Takashiba, S.
  • Journal Title: Dental Materials 25 Pages: 424-430
• [Journal Article] Effect of polyphosphoric-acid pre-treatment of titanium on attachment, proliferation and differentiation of osteoblast like cells(MC3T3-E1) (2008)
  • Author(s): Maekawa, K., Yoshida, Y., Mine, A., Van Meerbeek, B., Suzuki, K., Kuboki, T.
  • Journal Title: Clinical Oral Implants Research 19 Pages: 320-325
• [Journal Article] Promotion of hydroxyapatite-associated, stem cells-based bone regeneration by CCN2 (2008)
  • Author(s): Ono, M., Kubota, S., Fujisawa, T., Sonoyama, W., Kawaki, H., Akiyama, K., Oshima, M., Nishida, T., Yoshida, Y., Suzuki, K., Takigawa, M., Kuboki, T.
  • Journal Title: Cell Transplantation 17 Pages: 231-240
• [Journal Article] Influence of the chemical structure of functional monomers on their adhesive performance (2008)
  • Author(s): Van Landuyt, K.L., Yoshida, Y., Hirata, I., Snauwaert, J., De Munck, J., Okazaki, M., Suzuki, K., Lambrechts, P., Van Meerbeek, B.
  • Journal Title: Journal of Dental Research 87 Pages: 757-761
• [Journal Article] Modification of the titan surface with photoreactive gelatin to regulate cell attachment (2007)
  • Author(s): Heydari, M., Hasuda, H., Sakuragi, M., Yoshida, Y., Suzuki, K., Ito, Y.
  • Journal Title: Journal of Biomedical Materials Research : Part A 83 Pages: 906-914
• [Journal Article] Chemical interaction of polyphosphoric acid with titanium and its effect on human bone marrow derived mesenchymal stem cell behavior (2007)
  • Author(s): Maekawa, K., Yoshida, Y., Mine, A., Fujisawa, T., Van Meerbeek, B., Suzuki, K., Kuboki, T.
  • Journal Title: J Materials Research : Part A 82A Pages: 195-200
• [Journal Article] Promotion of attachment of human bone marrow stromal cells by CNN2 (2007)
  • Author(s): Ono, M., Kubota, S., Fujisawa, T., Sonoyama, W., Kawaki, H., Akiyama, K.,Oshima, M., Nishida, T., Yoshida, Y., Suzuki, K., Takigawa, M., Kuboki, T.
  • Journal Title: Biochemical and Biophysical Research Communications 357 Pages: 20-25
• [Journal Article] Gel phase formation at resin-modified glass-ionomer/tooth interfaces (2007)
  • Author(s): Coutinho, E., Yoshida, Y., Inoue, S., Fukuda, R., Snauwaert, J., Nakayama, Y., De Munck, J., Lambrechts, P., Suzuki, K., Van Meerbeek, B.
  • Journal Title: Journal of Dental Research 86 Pages: 656-661
• [Journal Article] Origin of interfacial droplets self-etch adhesives (2007)
  • Author(s): Van Landuyt, K.L., Snauwaert, J., De Munck, J., Coutinho, E., Poitevin, A., Yoshida, Y., Suzuki, K., Lambrechts, P., Van Meerbeek. B.
  • Journal Title: Journal of Dental Research 86 Pages: 739-744
• [Journal Article] Modification of the titan surface with photoreactive gelatin to regulate cell attachment. (2007)
  • Author(s): Heydari, M., Hasuda, H., Sakurag
  • Journal Title: Journal of Biomedical Materials Research: Part A 83 Pages: 906-914
  • Peer Reviewed
• [Journal Article] Effect of 4-MET-and 10-MDP-based priresin bonding to titanium (2006)
  • Author(s): Tsuchimoto, Y., Yoshida, Y., Mine, A., Nakamura, M., Nishiyama, N., Van Meerbeek, B., Suzuki, K
  • Journal Title: Dental Materials Journal 25 Pages: 120-124
• [Journal Article] Effect of surface pre-treatment on durability of resin-based cements bonded to titanium (2006)
  • Author(s): Tsuchimoto, Y., Yoshida, Y., Takeuchi, M., Mine, A., Yatani, H., Tagawa, Y., Van Meerbeek, B., Suzuki, K., Kuboki, T.
  • Journal Title: Dental Materials 22 Pages: 545-552
• [Journal Article] Chemical interaction of phosphoric acid ester with hydroxyapatite (2006)
  • Author(s): Fukegawa, D., Hayakawa, S., Yoshida, Y., Suzuki, K., Osaka, A.,Van Meerbeek, B.
  • Journal Title: Journal of Dental Research 85 Pages: 941-944
• [Presentation] Strates to create the super tooth (2009)
  • Author(s): Yoshida, Y.
  • Organizer: Nano-interfacial control for functional reconstruction of human hard tissues Global Center of Excellence Program ; International Research Center for Molecular Science in Tooth and Bone Diseases : G-COE 1st International Symposium
  • Place of Presentation: Tokyo Gakusei Kaikan, Tokyo, Japan
  • Year and Date: 2009-01-22
• [Presentation] Tissue-Biomaterial Interface Research for Prosthodontic Dentistry (2008)
  • Author(s): Yoshida, Y.
  • Organizer: Interfacial control for functional reconstruction of human hard tissues. The International Symposium of the 117th Scientific Meeting of Japan Prosthodontic Society in Nagoya, The International Congress of Prosthodontics 2008 in Nagoya, The 1st Biennial Joint Congress of Chinese Prosthodontic Society, Japan Prosthodontic Society and Korean Academy of Prosthodontics
  • Place of Presentation: Nagoya Congress Center, Nagoya, Japan
• [Presentation] Nano-interfacial control for functional reconstruction of human hard tissues. The 9th International Symposium on Biomimetic Interfaces (2007)
  • Author(s): Yoshida, Y.
  • Organizer: Recent trends of nanobiotechnology in medical and dental research
  • Place of Presentation: Seoul National University, Seoul, Korea
  • Year and Date: 2007-02-01
• [Presentation] Nano-interfacial control for hard tissue reconstruction. (2007)
  • Author(s): Yoshida, Y.
  • Organizer: The Annual Scientific Meeting of the Association for Dental Sciences of the Republic of China
  • Place of Presentation: The TaiPei International Convention Center, Taipei, Taiwan.
• [Presentation] Surface modification of Ti implants for promotion of bone regeneration (2007)
  • Author(s): Yoshida, Y., Suzuki, K.
  • Organizer: The 6th International Symposium on Titanium in Dentistry. International Conference Center
  • Place of Presentation: Kyoto, Japan
• [Presentation] 光反応性ゼラチンによるチタン表面の生物学的改質 (2006)
  • Author(s): 吉田靖弘, 鈴木一臣
  • Organizer: 第48回日本歯科理工学会学術講演会
  • Place of Presentation: 名古屋
• [Remarks] 第48回日本歯科理工学会学術講演会発表優秀賞(2007年)