Reduction of oral carriage of periodontal bacteria by the use of the induction of beta defensin through molecular pattern recognition into saliva
| Project/Area Number |
18390516
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
補綴理工系歯学
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| Research Institution | Hiroshima University |
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Principal Investigator |
NIKAWA Hiroki Hiroshima University, Faculty of Dentistry, Professor (10228140)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2007: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
Fiscal Year 2006: ¥10,400,000 (Direct Cost: ¥10,400,000)
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| Keywords | saliva / beta defensin / periodontal bavteria / citric acid / βデフェンシン / ガム / クエン酸 |
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| Research Abstract |
Objective: In a preliminary study, we noted that citric acid has the potency to induce human beta defensins (hBD-2) in salivary secretions. As hBDs are thought to ameliorate periodontal disease by suppressing key periodontal pathogens we developed a citric acid-containing chewing gum (CA-gum) to test this hypothesis. Here we report, the effect of CA-gum on three major periodontal pathogens Prevotella intermedia (Pi), Tannerella forsythia (Tf), Treponema denticola (Td) and Fusobacterium species (Fb).
Methods: A randomized, double-blind, placebo-controlled trial with 61 individuals, was conducted over 14 days. Each subject was allowed to chew CA-gum (31 subjects) or placebo gum (30 subjects) at irregular intervals, but was instructed to eat at least three pieces of gum a day. None had active caries, symptoms of gingivitis or periodontal disease, smoking history, or concurrent medication. To determine the levels of oral carriage of the putative pathogens, saliva was collected from each subject by paraffin chewing in a standard manner and, the number of total cultivative microorganisms (CFUs) determined. Five key periodontal pathogens, Pg, Pi, TI Td and Fb present in saliva were determined quantitatively using real-time polymerase chain reaction (PCR) (BML Inc., Saitama, JAPAN).
Results: Compared with the placebo chewing gum group the CA-gum group had significantly lower levels of Prevotella intermedia, Tannerella forsythia, and Fusobacterium spp. in their saliva. (Wilcoxon t-test; p<0.01). However, chewing of either type of gum did not affect the salivary carriage of total cultivative bacteria (Wilcoxon t-test; p>0.05). Conclusion: Consumption of citric acid-laced chewing gum reduces significantly the burden of periodontal pathogens in saliva.
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Report
(3 results)
Research Products
(4 results)
