Development of high-turnover bone substitutes using hydrothermally synthesized 8-TCP composed of rod-shaped particles and RANKL

• Project/Area Number: 18390519
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: 補綴理工系歯学
• Research Institution: Nagasaki University
• Principal Investigator: IKEDA Tohru Nagasaki University, 大学院・医歯薬学総合研究科, 教授 (00211029)
• Co-Investigator(Kenkyū-buntansha): IOKU Koji 東北大学, 大学院・環境科学研究科, 教授 (60212726) ; YONEZAWA Ikuho 順天堂大学, 医学部, 准教授 (80327858) ; SHIBATA Yasuaki 長崎大学, 大学院・医歯薬学総合研究科, 助教 (80253673) ; SAWASE Takashi 長崎大学, 大学院・医歯薬学総合研究科, 教授 (80253681) ; FUJITA Shuichi 長崎大学, 大学院・医歯薬学総合研究科 (00181355) ; 熱田 充 長崎大学, 大学院・医歯薬学総合研究科, 教授 (60002135)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥18,100,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥2,700,000); Fiscal Year 2008: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2007: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2006: ¥6,400,000 (Direct Cost: ¥6,400,000)
• Keywords: 骨代替材料 / β-リン酸三カルシウム / ハイドロキシアパタイト / 破骨細胞 / 骨移植 / 骨代謝 / 骨吸収 / 骨芽細胞 / 骨形態計測 / 培養細胞 / 不動化 / 細胞培養 / カルシウム

Research Abstract

本研究課題では、独自の水熱法にて開発した柱状粒子セラミックスを用いて自家骨移植に代わる高機能な骨代替材料を開発することを目指す。水熱法にてハイドロキシアパタイト(HA)及びβ-TCPのブロック及び球状顆粒を作製し、家兎とラットの大腿骨に移植した。HAは生体内吸収性を示し、かつ周囲に多量の骨を形成した。一般にHAは生体内非吸収性であり、本研究で世界初の生体内吸収性HAを作製した。また、移植物周囲の骨形成に破骨細胞が直接関与していることを細胞培養実験で示した。

Research Products

• [Journal Article] Osteoconductivity of hydrothermally synthesized beta-tricalcium phosphate composed of rod-shaped particles under mechanical unloading (2009)
  • Author(s): Gonda, Y., Ioku, K., Okuda, T., Shibata, Y., Kamitakaraha, M., Kawachi, G., Yonezawa, I., Kurosawa, H., and Ikeda, T
  • Journal Title: Key Eng. Mater 396-398 Pages: 253-256
  • NAID: 120006983344
  • Peer Reviewed
• [Journal Article] Calcium phosphate porous materials with unique microstructures (2009)
  • Author(s): Ioku, K., Kamitakahara, M., Watanabe, N., Kawaguchi, O., Murakami, S., and Ikeda, T
  • Journal Title: Key Eng. Mater 396-398 Pages: 645-648
  • Peer Reviewed
• [Journal Article] Osteoconductivity of hydrothermally synthesized beta-tricalcium phosphate composed of rod-shaped particles under mechanical unloading (2009)
  • Author(s): Gonda Y, Ioku K, (6名), Ikeda T
  • Journal Title: Key Eng. Mater. 396-398 Pages: 253-256
  • Peer Reviewed
• [Journal Article] Calcium phosphate porous materials with unique microstructures (2009)
  • Author(s): Ioku K, Kamitakahara M, (3名), Ikeda T
  • Journal Title: Key Eng. Mater. 396-398 Pages: 645-648
  • Peer Reviewed
• [Journal Article] The slow resorption with replacement by bone of a hydrothermally synthesized pure calcium-deficient hydroxyapatite (2008)
  • Author(s): Okuda, T., loku, K., Yonezawa, I., Minagi, H., Gonda, Y., Kawachi, G., Kamitakahara, M., Shibata, Y., Murayama, H., Kurosawa, H., Ikeda, T
  • Journal Title: Biomaterials 29 Pages: 2719-2728
  • Peer Reviewed
• [Journal Article] β-TCPの粒子構造と生体反応 (2008)
  • Author(s): 池田通、井奥洪二
  • Journal Title: Ceramics Japan 43 Pages: 294-297
  • NAID: 10021138427
• [Journal Article] Application of newly developed globular-shaped granules of beta-tricalcium phosphate for bone substitute (2008)
  • Author(s): Gonda, Y., loku, K., Okuda, T., Kawachi, G., Yonezawa, I., Kurosawa, H., Ikeda, T.
  • Journal Title: Key Eng. Mater 361-363 Pages: 1013-1016
  • Peer Reviewed
• [Journal Article] Microstructure designing of porous f3-tricalcium phosphate for control of reactions in the bone (2008)
  • Author(s): Ioku, K., Kamitakahara, M., Kawachi, G., Gonda, Y., Okuda, T., Yonezawa, I., Kurosawa, H., Ikeda, T.
  • Journal Title: Key Eng. Mater 361-363 Pages: 989-992
  • Peer Reviewed
• [Journal Article] The slow resorption and replacement by bone of a hydrothermally-synthesized pure calcium-deficient hydroxyapatite (2008)
  • Author(s): Okuda T, Ioku K, Yonezawa I, Minagi H, (6名), Ikeda T
  • Journal Title: Biomaterials 29 Pages: 2719-2728
  • Peer Reviewed
• [Journal Article] β-TCPの兼子構造と生体反応 (2008)
  • Author(s): 池田 通, 井奥洪二
  • Journal Title: Ceramics Japan 43 Pages: 294-297
• [Journal Article] Application of newly developed globular-shaped granules of beta-tricalcium phosphate for bone substitute (2008)
  • Author(s): Gonda Y, Ioku K, Okuda T, Kawachi G, Yonezawa I, Kurosawa H, Ikeda T
  • Journal Title: Key Eng.Mater. 361-363 Pages: 1013-1016
• [Journal Article] Microstructure designing of porous β-tricalcium phosphate for control of reactions in the bone (2008)
  • Author(s): Ioku K, Kamitakahara M, Kawachi G, Gonda Y, Okuda T, Yonezawa I, Kurosawa H, Ikeda T
  • Journal Title: Key Eng.Mater. 361-363 Pages: 989-992
• [Journal Article] The slow resorption and replacement by bone of a hydrothermally-synthesized pure calcium-deficient hydroxyapatite (2008)
  • Author(s): Okuda T, Ioku K, Yonezawa I, Minagi H, (6名), Ikeda T
  • Journal Title: Biomaterials (In press)
  • Peer Reviewed
• [Journal Article] The effect of the microstructure of β-tricalcium phosphate on the metabolism of subsequently formed bone tissue (2007)
  • Author(s): Okuda, T., Ioku, K., Yonezawa, I., Minagi, H., Kawachi, G., Gonda, Y. , Murayama, H., Shibata, Y., Minami, S., Kamihira, S., Kurosawa, H., Ikeda, T.
  • Journal Title: Biomaterials 28 Pages: 2612-2621
  • Peer Reviewed
• [Journal Article] The effect of microstructure of β-tricalcium phosphate on the metabolism of subsequently formed bone tissue (2007)
  • Author(s): Okuda T, Ioku K, Yonezawa I, Mingagi H, (7名), Ikeda T
  • Journal Title: Biomaterials 28 Pages: 2612-2621
  • Peer Reviewed
• [Journal Article] The effect of microstructure of β-tricalcium phosphate on the metabolism of subsequently formed bone tissue (2007)
  • Author(s): Okuda T, Ioku K, Yonezawa I, Mingagi H, (7名), Ikeda T
  • Journal Title: Biomaterials In press
• [Presentation] 柱状粒子β-リン酸三カルシウム球状顆粒の不動化骨組織における吸収と骨伝導 (2008)
  • Author(s): 権田芳範、奥田貴俊、井奥洪二、柴田恭明、米澤郁穂、黒澤尚、池田通
  • Organizer: 第26回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2008-10-30
• [Presentation] Osteoconductivity of hydrothermally synthesized beta-tricalcium phosphate composed of rod-shaped particles under mechanical unloading (2008)
  • Author(s): Gonda, Y., Ioku, K., Okuda, T., Shibata, Y., Kamitakaraha, M., Kawachi, G., Yonezawa, I., Kurosawa, H., and Ikeda, T.
  • Organizer: Bioceramics 21
  • Place of Presentation: Buzios, Brazil
  • Year and Date: 2008-10-25
• [Presentation] Calcium phosphate porous materials with unique microstructures (2008)
  • Author(s): Ioku, K., Kamitakahara, M., Watanabe, N., Kawaguchi, O., Murakami, S., and Ikeda, T.
  • Organizer: Bioceramics 21
  • Place of Presentation: Buzios, Brazil
  • Year and Date: 2008-10-24
• [Presentation] 柱状粒子ハイドロキシアパタイト球状顆粒の骨組織への移植による吸収と骨伝導 (2008)
  • Author(s): 権田芳範、柴田恭明、井奥洪二、池田通
  • Organizer: 第50回歯科基礎医学会総会
  • Place of Presentation: 東京
  • Year and Date: 2008-09-24
• [Presentation] Application of newly developed globular-shaped granules of beta-tricalcium phosphate for bone substitute (2007)
  • Author(s): Ikeda, T., Gonda, Y., Ioku, K., Okuda, T., Kawachi, G., Yonezawa, I., Kurosawa, H.
  • Organizer: Bioceramics 20
  • Place of Presentation: Nantes, France
  • Year and Date: 2007-10-26
• [Presentation] Microstructure designing of porous β-tricalcium phosphate for control of reactions in the bone (2007)
  • Author(s): Ioku, K., Kamitakahara, M., Kawachi, G., Gonda, Y., Okuda, T., Yonezawa, I., Kurosawa, H., Ikeda, T
  • Organizer: Bioceramics 20
  • Place of Presentation: Nantes. France
  • Year and Date: 2007-10-26
• [Presentation] 柱状粒子ハイドロキシアパタイトからなる新しい骨代替材料の開発と評価 (2007)
  • Author(s): 奥田貴俊, 井奥洪二, 権田芳範, 米澤郁穂, 黒澤尚, 池田通
  • Organizer: 第22回日本整形外科学会基礎学術集会
  • Place of Presentation: 浜松
  • Year and Date: 2007-10-26
• [Presentation] Application of newly developed globular-shaped granules of beta-tricalcium phosphate for bone substitute (2007)
  • Author(s): Gonda Y, Ioku K, Okuda T, Kawachi G, Yonezawa I, Kurosawa H, Ikeda T
  • Organizer: Bioceramics 20
  • Place of Presentation: Nantes,France
  • Year and Date: 2007-10-26
• [Presentation] Microstructure designing of porous β-tricalcium phosphate for control of reactions in the bone (2007)
  • Author(s): Ioku K, Kamitakahara M, Kawachi G, Gonda Y, Okuda T, Yonezawa I, Kurosawa H, Ikeda T
  • Organizer: Bioceramics 20
  • Place of Presentation: Nantes,France
  • Year and Date: 2007-10-25
• [Presentation] 骨代替材料の吸収性と新生骨形成の関係に対する評価, (2007)
  • Author(s): 奥田貴俊, 井奥洪二, 米澤郁穂, 権田芳範, 柴田恭明, 黒澤尚, 池田通
  • Organizer: 第25回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2007-07-19
• [Presentation] 微細柱状粒子β-リン酸三カルシウム球状穎粒の開発と骨代替材料としての評価, (2007)
  • Author(s): 権田芳範, 奥田貴俊, 井奥洪二, 米澤郁穂, 黒澤尚, 柴田恭明, 池田通
  • Organizer: 第25回日本骨代謝学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2007-07-19
• [Presentation] 骨代替材料の吸収性と新生骨形成の関係に対する評価 (2007)
  • Author(s): 奥田 貴俊, 井奥 洪二, 米澤 郁穂, 権田 芳範, 柴田 恭明, 黒澤 尚, 池田 通
  • Organizer: 第25回日本骨代謝学会
  • Place of Presentation: 大阪
  • Year and Date: 2007-07-19
• [Presentation] 微細柱状粒子β-リン酸三カルシウム球状顆粒の開発と骨代替材料としての評価 (2007)
  • Author(s): 権田 芳範, 奥田 貴俊, 井奥 洪二, 米澤 郁穂, 黒澤 尚, 柴田 恭明, 池田 通
  • Organizer: 第25回日本骨代謝学会
  • Place of Presentation: 大阪
  • Year and Date: 2007-07-19
• [Presentation] 柱状結晶β-TCPからなる新しい骨代替材料の開発と評価 (2006)
  • Author(s): 奥田貴俊, 井奥洪二, 権田芳範, 米澤郁穂, 黒澤尚, 池田通
  • Organizer: 第21回日本整形外科学会基礎学術集会
  • Place of Presentation: 長崎
  • Year and Date: 2006-10-20
• [Presentation] 歯骨細胞培養系を用いた骨代替材料の吸収性の評価 (2006)
  • Author(s): 権田芳範, 井奥洪二, 奥田貴俊, 米澤郁穂, 黒澤尚, 池田通
  • Organizer: 第21回日本整形外科学会基礎学術集会
  • Place of Presentation: 長崎
  • Year and Date: 2006-10-20
• [Presentation] 新規吸収性骨代替材料微細柱状結晶β-リン酸三カルシウムの評価 (2006)
  • Author(s): 奥田貴俊, 権田芳範, 井奥洪二, 米澤郁穂, 黒澤尚, 柴田恭明, 池田通
  • Organizer: 第24回日本骨代謝学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2006-07-07
• [Presentation] Porous 13-tricalcium phosphate composed of rod-shaped particles (2006)
  • Author(s): Ioku, K., Minagi, H., Kawachi, G., Okuda, T., Yonezawa, I., Kurosawa, H., Ikeda, T.
  • Organizer: The sixth Asian Bioceramics Symposium
  • Place of Presentation: Bangkok
• [Book] 「多孔体の精密制御と機能・物性評価」スキャホールドとしての多孔質リン酸カルシウム (2008)
  • Author(s): 井奥洪二、池田通
  • Publisher: サイエンス&テクノロジー
• [Book] 「多孔体の精密制御と機能・物性評価」(共著)スキャホールドとしての多孔質リン酸カルシウム (2008)
  • Author(s): 井奥洪二, 池田 通
  • Publisher: サイエンス&テクノロジー