| Project/Area Number |
18390531
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Surgical dentistry
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| Research Institution | Hokkaido University |
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Principal Investigator |
SHINDOH Masanobu Hokkaido University, Graaduate School of Dental Medicine, Professor (20162802)
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| Co-Investigator(Kenkyū-buntansha) |
KOBAYASHI Masanobu Hokkaido University, Institute for Genetic Medicine, Associate Professeor (80241321)
FUGETSU Bunshi Hokkaido University, Graduate School of Environmental Science & Faculty of Environmetal Earth Science, Professor (40281844)
HIGASHINO Fumihiro Hokkaido University, Graduate School of Dental Medicine, Associate Professor (50301891)
HIDA Kyoko Hokkaido University, Graduate School of Dental Medicine, Assistant Professor (40399952)
KITAMURA Tetsuya Hokkaido University, Graduate School of Dental Medicine, Research Associate (00451451)
大廣 洋一 北海道大学, 大学院歯学研究科, 助手 (40301915)
戸塚 靖則 北海道大学, 大学院歯学研究科, 教授 (00109456)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥16,810,000 (Direct Cost: ¥15,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2007: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2006: ¥12,000,000 (Direct Cost: ¥12,000,000)
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| Keywords | nanocoloid / cell-specific gene transfer / adrenomedullin / 遺伝子導入 / EGFレセプター / 口腔扁平上皮がん / ナノテクノロジー |
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| Research Abstract |
Trials of gene therapies have been carried out for malignant tumors and congenital gene disorders,; however, there are problems such as the low efficiency and risk for applying human being, and ideal methods of gene therapy are not yet developed.. We designed nanocoloid that enclosed expression plasmid inside and, ligand peptide outside of small particle for development of a specific gene delivery system. When We searched expression of EGFR in oral squamous cell carcinoma cell lines (9.22, HSC2, HSC3, HSC4, Ca SAS) by Western blot, and all cell lines expressed EGFR We examined the binding affinity of EGFR and synthesized EGF peptide by BireCore X. As a result, it became clear that synthesized peptide bound to EGFR. Next, we examined the induction effect of nanocoloid to oral carcinoma cell lines. The efficiency of transfection was approximately 30%, and we aimed to improve the efficiency. We next examined the effects of adrenomedullin antagonist that has the ability to inhibit angiogenesis. The vector that has the sequence for inhibitory effects on aderenomedullin reduced the Growth of tumor xenografts in nude mice.
These results imply that specific gene delivery for tumor environment could be the therapeutic agents for solid cancers.
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