Analysis of oral diseases using oxidative stress inducible protein conditional knockout mouse

• Project/Area Number: 18390534
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: University of Tsukuba
• Principal Investigator: YOSHIDA Hiroshi University of Tsukuba, 大学院・人間総合科学研究科, 教授 (80014330)
• Co-Investigator(Kenkyū-buntansha): ISHII Tetsuro 筑波大学, 大学院・人間総合科学研究科, 教授 (20111370) ; YANAGAWA Toru 筑波大学, 大学院・人間総合科学研究科, 講師 (10312852)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥18,340,000 (Direct Cost: ¥15,400,000、Indirect Cost: ¥2,940,000); Fiscal Year 2008: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000); Fiscal Year 2007: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2006: ¥5,600,000 (Direct Cost: ¥5,600,000)
• Keywords: A170 / Peroxiredoxin I / p62 / 酸化ストレスタンパク質 / PeroxiredoxinI

Research Abstract

口腔病変の解析をするために、組織特異的欠損(コンディッショナルノックアウト : CKO)マウスを制作と同時に、既存のmull ノックアウト(KO)マウスを用いて以下のように研究をおこなった。 (1) CKO マウスの制作 : A170遺伝子にLoxPで第一エクソンを挟んだflox/floxマウスを制作し、Creマウスと掛け合わせてCKO マウスを完成させた。(2)口腔病変における酸化ストレスの意義の解析 : Peroxiredoxin I遺伝子KOマウスを用いて、シスプラチンの感受性の低下について解析した。また、A170遺伝子KOマウスのフェノタイプに解析を追加した。

Research Products

• [Journal Article] The α -glucosidase inhibitor acarboseprevents obesity and simple steatosis insequestosome 1/A170/p62 deficient mice (2009)
  • Author(s): Kosuke Okada, Toru Yanagawa EijiWarabi, Keiko Yamastu, Junya Uwayama,Koichi Takeda, Hirotoshi Utsunomiya,Hiroshi Yoshida, Junichi Shoda and Tetsuro Ishii
  • Journal Title: Hepatol Res (in press)
  • Peer Reviewed
• [Journal Article] The alpha-glucosidase inhibitor acarbose prevents obesity and simple steatosis in sequestosome 1/A170/p62 deficient mice. (2009)
  • Author(s): Okada K, et. al.
  • Journal Title: Hepatol Res (in press) 39(5) Pages: 490-500
  • Peer Reviewed
• [Journal Article] omeostatic levels of p62 controlcytoplasmic inclusion body formationin autophagy-deficient mice (2007)
  • Author(s): Komatsu M, Waguri S, Koike M, Sou YS,Ueno T, Hara T, Mizushima N, Iwata J, Ezaki J, Murata S, Hamazaki J, Nishito Y, Iemura S, Natsume T, Yanagawa T,Uwayama J, Warabi E, Yoshida H, Ishii T,Kobayashi A, Yamamoto M, Yue Z,Uchiyama Y, Kominami E, Tanaka K.
  • Journal Title: Cell. 131(6) Pages: 1149-63
  • Peer Reviewed
• [Journal Article] Homeostatic levels of p62 control cytoplasmic inclusion body formation in autophagy-deficient mice. (2007)
  • Author(s): Komatsu M, et. al.
  • Journal Title: Cell 131 Pages: 149-63
  • Peer Reviewed
• [Book] Subcellular Blochemistry series. Peroxiredoxin Systems : Structures and Functions Chapter 18. Stress-induced Peroxiredoxins
  • Author(s): Tetsuro Ishii
  • Publisher: Springer (in press)