Elucidation of condylar cartilage resorption mechanism in TMJ-osteoarthritis and search of a new regulatory factor

• Project/Area Number: 18390554
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Orthodontic/Pediatric dentistry
• Research Institution: Hiroshima University
• Principal Investigator: TANNE Kazuo Hiroshima University, Graduate School of Biomedical Sciences, Professor (30159032)
• Co-Investigator(Kenkyū-buntansha): TANIMOTO Koutaro Hiroshima University, Graduate School of Biomedical Sciences, Assitant Professor (20322240) ; TANAKA Nobuaki Hiroshima University, Graduate School of Biomedical Sciences, Assitant Professor (90397969) ; 本田 康文 広島大学, 大学院医歯薬学総合研究科, 助手 (00335663)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥16,390,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥2,190,000); Fiscal Year 2007: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000); Fiscal Year 2006: ¥6,900,000 (Direct Cost: ¥6,900,000)
• Keywords: mandibular condyle / SZP / Hyaluronic acid / Hyaluronidase / Mechanical stress / ヒアルロン酸 / ヒアルロニダーゼ

Research Abstract

The TMJ- osteoarthritis (OA) of the condylar cartilage absorption with movement disorder and dysfunction is one of the disease that are difficult for treatment in dental field Excessive mechanical loading to be added to the condyle as the muse is thought about with key factor of onset of OA On the other hand, it is speculated that the cartilage destruction is induced by an unknown new factor in synergy with a mechanical loading, bemuse there is the case that OA progress in a normal physiological condition. Mandibular condyle is composed of several different zone, as superficial zone from surface, cartilaginous layer, subchondral bone and bone, each layer has a different function and respond to various mechanical irritation by jaw movement Superfical zone has a lubrication function to decrease the friction, and a bone underlying in the cartilaginous layer supports the above tissues rigidly. In order to elucidate an inducing mechanism of metabolic abnormality in each layer it is importan t for clarifying the mechanisms of condylar cartilage destruction. We confirmed the expression of superficial zone protein which had boundary lubrication function in superficial zone of condylar cartilage in 1118 year and clarified the expression level was regulated selectively by cytokine and the magnitude of mechanical loading. In this study, we particularly focused on the downward layer (subchondral bone), and investigated the influences of subchondral bone damaged with excessive mechanical loading on the metabolisim of condylar cartilage. As a result of a 200 grams metal object was dropped to the mandibular condyle as a high magnitude impact loading, in particular, the expression of IL -1β was found in subchondral bone immunohistologically. Next, to investigate interactions between osteoblasts and chondrocytes, we used a co-culture system to culture osteoblasts monolayer together with chondrocytes in alginate beads. The mRNA expression of MMP-1, 3 and 13 in the chondrocytes by co-culture with mechanical stress-loaded osteoblasts showed increased, and the mRNA expression type II, X collagen and DNA, proteoglycan syntheses were decreased- From these results, the aggression to mandibular condyle head from the outside induce IL -1 β from subchondral bone. The results from the present study suggest that physical interactions between subchondral osteoblasts and articular chondrocytes could be responsible for the cartilage degradation in pathological changes of proceeding OA.

Research Products

• [Journal Article] Inhibition of proliferation of human periodontal ligament fibroblasts by hyaluronidase (2008)
  • Author(s): Tanimoto K, Nakatani Y, Tanaka N, Ueki M, Yanagida T, Kitamura R, Tanne Y, Lin YY, Kunimatsu R, Tanne K.
  • Journal Title: Archives of oral biology 53 Pages: 330-336
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] The effect of mechanical loading on the metabolism of growth plate chondrocyte (2008)
  • Author(s): Ueki M, Tanaka N, Tanimoto K, Nishio C, Honda K, Lin YY, Tanne Y, Ohkuma S, Kamiya T, Tanaka E, Tanne K.
  • Journal Title: Annals of biomedical engineering 36 Pages: 793-800
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Expression and activity of Runx2 mediated by hyaluronan during chondrocyte differentiation (2008)
  • Author(s): Tanne Y, Tanimoto K, Tanaka N, Ueki M, Lin YY, Ohkuma S, Kamiya T, Tanaka E, Tanne K
  • Journal Title: Archives of oral biology 53 Pages: 478-487
  • NAID: 120001041390
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Lubrication of the temporomandibular joint (2008)
  • Author(s): Tanaka E, Detamore MS, Tanimoto K, Kawai N.
  • Journal Title: Annals of biomedical engineering 36 Pages: 14-29
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Lubrication of the temporomandibular joint (2008)
  • Author(s): Tanaka, E., Detamore, MS., Tanimoto, K., Kawai, N
  • Journal Title: Ann Biomed Eng 36 Pages: 14-29
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Inhibition of the proliferation of human periodontal ligament fibroblasts by hyaluronidase (2008)
  • Author(s): Tanimoto, K., Nakatani, Y., Tanaka, N., Ueki, M., Yanagida, T., Kitamura, R., Tanne, Y., Lin, YY., Kunimatsu, R., Tanne
  • Journal Title: Arch Oral Biol 53 Pages: 330-336
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The effect of mechanical loading on the metabolism of growth plate chondrocytes (2008)
  • Author(s): Ueki, M., Tanaka, N., Tanimoto, K., Nishio, C., Honda, K., Lin, YY., Tanne, Y., Ohkuma, S., Kamiya, T., Tanaka, E., Tanne, K
  • Journal Title: Ann Biomed Eng 36 Pages: 793-800
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expression and activity of Runx2 mediated by hyaluronan during chondrocyte differentiation (2008)
  • Author(s): Tanne, Y., Tanimoto, K., Tanaka, N., Ueki, M., Lin, YY., Ohkuma, S., Kamiya, T., Tanaka, E., Tanne, K
  • Journal Title: Arch Oral Biol 53 Pages: 478-487
  • NAID: 120001041390
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] The effect of mechanical loading on the metabolism of growth plate chondrocyte (2008)
  • Author(s): Ueki M, Tanaka N, Tanimoto K, Nishio C, Honda K, Lin YY, Tanne Y, Ohkuma S, Kamiya T, Tanaka E, Tanne K.
  • Journal Title: Annals of biomedical engineering (In press)
  • Peer Reviewed
• [Journal Article] Expression and activity of Runx2 mediated by hyaluronan during chondrocyte differentiation (2008)
  • Author(s): Tanne Y, Tanimoto K, Tanaka N, Ueki M, Lin YY, Ohkuma S, Kamiya T, Tanaka E, Tanne K.
  • Journal Title: Archives of oral biology (In press)
  • NAID: 120001041390
  • Peer Reviewed
• [Journal Article] Lubrication of the temporomandibular joint (2008)
  • Author(s): Tanaka E, Detamore MS, Tanimoto K, Kawai N.
  • Journal Title: Ann Biomed Eng. 36(1) Pages: 14-29
  • Peer Reviewed
• [Journal Article] Expression of superficial zone protein in mandibular condyle-cartilage (2006)
  • Author(s): Ohno S, Schmid T, Tanne Y, Kamiya T, Honda K, Ohno-Nakahara M, Swentko N, Desai TA, Tanne K, Knudson CB, Knudson W.
  • Journal Title: Osteoarthritis Cartilage 14 Pages: 807-813
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Expression of superficial zone protein in mandibular condyle cartilage (2006)
  • Author(s): Ohno, S., Schmid, T., Tanne, Y., Kamiya, T., Honda, K., Ohno-Nakahara, M., Swentko, N., Desai, T., Tanne, K., Knudson, C., Knudson, W
  • Journal Title: Osteoarthritis Cartilage 14 Pages: 807-813
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Expression of superficial zone protein in mandibular condyle cartilage. (2006)
  • Author(s): Ohno S.
  • Journal Title: Osteoarthritis Cartilage 14・8 Pages: 807-13
• [Presentation] 滑膜細胞におけるヒアルロン酸分解に対する炎症性サイトカインの影響 (2007)
  • Author(s): 谷本幸太郎、田中伸明、植木雅士、柳田珠美、北村玲子、丹根由起、林于郁、神谷貴志、大熊 暁、黄 郁チン、国松 亮、吉岡基子、丹根一夫
  • Organizer: 第55回JADR総会・学術大会
  • Place of Presentation: 横浜市
  • Year and Date: 2007-11-18
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Effects of proinflammatory cytokines on the degradation of hyaluronan in synovial cells (2007)
  • Author(s): Tanimoto, K., Tanaka, N., Ueki, M., Yanagida, T., Kitamura, R., Tanne, Y., Lin, YY., Kamiya, T., Ohkuma, S., Huang YC., Kunimatsu, R., Yoshioka, M., Tanne, K
  • Organizer: The 55th Annual Meeting of Japanese Association for Dental Reserch
  • Place of Presentation: Yokohama
  • Year and Date: 2007-11-18
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Effects of hyaluronan oligosaccharide on MMP expression in gingival fibroblasts (2007)
  • Author(s): Ohkuma, S., Tanimoto, K., Tanaka, N., Ueki, M., Kitamura, R., Yanagida, T., Tanne, Y., Lin, YY., Kamiya, T., Kunimatsu, R., Huang, YC., Yoshioka, M., Tanne, K
  • Organizer: The 55th Annual Meeting of Japanese Association for Dental Research
  • Place of Presentation: Yokohama
  • Year and Date: 2007-11-18
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Effects of Amelogenin on the Metabolism of Periodontal Tissue Cells (2007)
  • Author(s): Kunimatsu, R., Tanimoto, K., Tanaka, N., Ueki, M., Kitamura, R., Yanagida, T., Tanne, Y., Lin, YY., Kamiya, T., Ohkuma, S., Huang, YC., Yoshioka, M., Tanne, K
  • Organizer: The 55th Annual Meeting of Japanese Association for Dental Research
  • Place of Presentation: Yokohama
  • Year and Date: 2007-11-18
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 培養ウサギ滑膜細胞と軟骨細胞のヒアルロン酸代謝に対する機械的負荷の影響 (2007)
  • Author(s): 北村玲子、谷本幸太郎、田中伸明、植木雅士、柳田珠美、丹根由起、神谷貴志、丹根一夫
  • Organizer: 第20回日本顎関節学会総会・学術大会
  • Place of Presentation: 仙台市
  • Year and Date: 2007-07-16
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 関節組織における潤滑・緩衝物質代謝に対する流水圧の影響 (2007)
  • Author(s): 柳田珠美、 谷本幸太郎、田中伸明、植木雅士、 北村玲子、 丹根由起、神谷貴志、丹根一夫
  • Organizer: 第20回日本顎関節学会総会・学術大会
  • Place of Presentation: 仙台市
  • Year and Date: 2007-07-16
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 機械的圧縮力によるsuperficial zone proteinの発現調節 (2007)
  • Author(s): 神谷貴志、谷本幸太郎、田中伸明、植木雅士、北村玲子、柳田珠美、丹根由起、丹根一夫
  • Organizer: 第20回 日本顎関節学会総会・学術大会
  • Place of Presentation: 仙台市
  • Year and Date: 2007-07-16
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 軟骨分化過程におけるRunx2活性および発現に対するヒアルロン酸の影響 (2007)
  • Author(s): 丹根 由起, 谷本 幸太郎, 田中 伸明, 植木 雅士, 北村 玲子, 柳田 珠美, 神谷 貴志, 丹根 一夫
  • Organizer: 第20回 日本顎関節学会総会・学術大会
  • Place of Presentation: 仙台市
  • Year and Date: 2007-07-16
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Influences of excessive cyclic tensile load on hyaluronan metabolism in cultured rabbit synoviocytes (2007)
  • Author(s): Kitamura, R., Tanimoto, K., Tanaka, N., Ueki, M., Yanagida, T., Tanne, Y., Kamiya, T., Tanne, K
  • Organizer: The 20th Annual Meeting of the Japanese Society for Temporomandibular Joint
  • Place of Presentation: Sendai
  • Year and Date: 2007-07-16
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Influences of fluid flow pressure on lubricant metabolism in joint tissue (2007)
  • Author(s): Yanagida, T., Tanimoto, K., Tanaka, N., Ueki, M., Kitamura, R., Tanne, Y., Kamiya, T., Tanne, K
  • Organizer: The 20th Annual Meeting of the Japanese Society for Temporomandibular Joint
  • Place of Presentation: Sendai
  • Year and Date: 2007-07-16
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Influences of hyaluronan on the expression and activity of Runx2 during chondrocyte differentiation (2007)
  • Author(s): Tanne, Y., Tanimoto, K., Tanaka, N., Ueki, M., Kitamura, R., Yanagida, T., Kamiya, T., Tanne, K
  • Organizer: The 20th Annual Meeting of Japanese Society for the Temporomandibular Joint
  • Place of Presentation: Sendai
  • Year and Date: 2007-07-16
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Effect of tensile stress on synthesis of superficial zone protein in chondrocytes (2007)
  • Author(s): Kamiya, T., Tanimoto, K., Tanaka, N., Ueki, M., Kitamura, R., Yanagida, T., Tanne, Y., Tanne, K
  • Organizer: The 20th Annual Meeting of the Japanese Society for the Temporomandibular Joint
  • Place of Presentation: Sendai
  • Year and Date: 2007-07-16
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 培養ウサギ滑膜細胞と軟骨細胞のヒアルロン酸代謝に対する機械的負荷の影響 (2007)
  • Author(s): 北村玲子、谷本幸太郎、田中伸明、植木雅士、柳田珠美、丹根由起、神谷貴志、丹根一夫
  • Organizer: 第20回 日本顎関節学会総会・学術大会
  • Place of Presentation: 仙台市
  • Year and Date: 2007-07-16
• [Presentation] 関節組織における潤滑・緩衝物質代謝に対する流水圧の影響 (2007)
  • Author(s): 柳田珠美、谷本幸太郎、田中伸明、植木雅士、北村玲子、丹根由起、神谷貴志、丹根一夫
  • Organizer: 第20回 日本顎関節学会総会・学術大会
  • Place of Presentation: 仙台市
  • Year and Date: 2007-07-16
• [Presentation] Subchondral bone damage alters the cartilage metabolism:mechanisms ofosteoarthritis (2007)
  • Author(s): Yu-Yu Lin, Nobuaki Tanaka, Masashi Ueki, Kotaro Tanimoto, Kazuo Tanne
  • Organizer: 第40回広島大学歯学会総会
  • Place of Presentation: 広島市
  • Year and Date: 2007-06-16
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Subchondral bone damage alters the cartilage metabolism : mechanisms of osteoarthritis (2007)
  • Author(s): Yu-Yu, Lin, Tanaka, N., Ueki, M., Tanimoto, K., Tanne, K
  • Organizer: The 40th Annual Meeting of the Hiroshima University Dental Society
  • Place of Presentation: Hiroshima
  • Year and Date: 2007-06-16
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Subchondral bone damage alters the cartilage metabolism: mechanisms of osteoarthritis (2007)
  • Author(s): Yu-Yu Lin, Nobuaki Tanaka, Masashi Ueki, Kotaro Tanimoto, Kazuo Tanne
  • Organizer: 第40回広島大学歯学会総会
  • Place of Presentation: 広島市
  • Year and Date: 2007-06-16
• [Presentation] Effects of fluid flow stress and hyaluronic acid on SZP expression in cultured TMJ chondrocytes (2006)
  • Author(s): Kamiya T., Honda K., Lin Y.Y., Tanne K.
  • Organizer: 第19回中華民國歯顎矯正學會
  • Place of Presentation: Taipei
  • Year and Date: 2006-12-14
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Effects of fluid flow stress and hyaluronic acid on SZP expression in cultured TMJ chondrocytes (2006)
  • Author(s): Kamiya, T., Honda, K., Lin, Y.Y., Tanne, K
  • Organizer: The 19th Taiwan Association of Orthodontics
  • Place of Presentation: Taipei
  • Year and Date: 2006-12-14
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 宿主年齢の異なる成長板軟骨細胞の増殖および分化に及ぼす機械的刺激の影響 (2006)
  • Author(s): 植木 雅士、田中 伸明、本田 康文、林 于郁、中谷 有希、北村 玲子、柳田 珠美、丹根 由起、大熊 暁、神谷 貴志、丹根 一夫
  • Organizer: 第65回日本矯正歯科学会大会
  • Place of Presentation: 札幌市
  • Year and Date: 2006-09-14
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 軟骨細胞の分化過程におけるRunx2活性に対するヒアルロン酸の影響 (2006)
  • Author(s): 丹根 由起、本田 康文、谷本 幸太郎、田中 伸明、米野 潔、植木 雅士、中谷 有希、北村 玲子、柳田 珠美、林 于郁、大熊 暁、神谷 貴志、丹根 一夫
  • Organizer: 第65回日本矯正歯科学会大会
  • Place of Presentation: 札幌市
  • Year and Date: 2006-09-14
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 滑膜性関節における潤滑物質代謝に対する化学的媒介物質および機械的負荷の影響 (2006)
  • Author(s): 柳田 珠美、本田 康文、谷本 幸太郎、田中 伸明、米野 潔、植木 雅士、中谷 有希、北村 玲子、丹根 由起、大熊 暁、神谷 貴志、丹根 一夫
  • Organizer: 第65回日本矯正歯科学会大会
  • Place of Presentation: 札幌市
  • Year and Date: 2006-09-14
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Effects of mechanical stimuli on the proliferation and differentiation of growth plate chondrocytes from different-aged hosts (2006)
  • Author(s): Ueki, M., Tanaka, N., Honda, K., Lin, Y.Y., Nakatani, Y., Kitamura, R., Yanagida, T., Tanne, Y., Ohkuma, S., Kamiya T., Tanne, K
  • Organizer: The 65th Annual Meeting of the Japanese Orthodontic Society
  • Place of Presentation: Sapporo
  • Year and Date: 2006-09-14
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Influences of hyaluronan on the activation of Runt, during chondrocyte differentiation (2006)
  • Author(s): Ueki, M., Tanaka, N., Honda, K., Lin, Y.Y., Nakatani, Y., Kitamura, R., Yanagida, T., Tanne, Y., Ohkuma, S., Kamiya, T., Tanne, K
  • Organizer: The 65th Annual Meeting of the Japanese Orthodontic Society
  • Place of Presentation: Sapporo
  • Year and Date: 2006-09-14
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Influences of chemical mediators and mechanical stress in synovial joint on lubricant metabolism (2006)
  • Author(s): Yanagida, Y., Honda, K., Tanimoto, K., Tanaka, N., Yoneno, K., Ueki, M., Nakatani, Y., Kitamura, R., Tanne, Y., Ohkuma, S., Kamiya, T., Tanne, K
  • Organizer: The 65th Annual Meeting of the Japanese Orthodontic Society
  • Place of Presentation: Sapporo
  • Year and Date: 2006-09-14
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Runx2 activation by hyaluronan during chondrocyte differentiation (2006)
  • Author(s): Tanne Y., Ohno S., Honda K., Tanimoto K., Tanaka N., Ohno-Nakahara M., Yoneno K., Ueki M., Nakatani Y., Kitamura R., Yanagida T., Lin Y.Y., Ohkuma S., Kamiya T., Tanne K.
  • Organizer: 84th General Session & Exhibition of the IADR
  • Place of Presentation: Brisbane
  • Year and Date: 2006-06-29
  • Description: 「研究成果報告書概要(和文)」より