International Research for Endocrine Disruption in Fish and Construction of Researcher Network in East Asia

• Project/Area Number: 18405003
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Risk sciences of radiation/Chemicals
• Research Institution: Nagasaki University
• Principal Investigator: SOYANO Kiyoshi Nagasaki University, 環東シナ海海洋環境資源研究センター, 教授 (80260735)
• Co-Investigator(Kenkyū-buntansha): NAGAE Masaki 長崎大学, 環境科学部, 准教授 (00315227) ; AOKI Jyun-ya 長崎大学, 環東シナ海海洋環境資源研究センター, 研究員 (20432990) ; TAKAO Yuji 長崎大学, 環境科学部, 准教授 (20206709) ; ISHIMATSU Atsushi 長崎大学, 環東シナ海海洋環境資源研究センター, 教授 (00184565) ; HARA Akihiko 北海道大学, 大学院・水産科学研究科, 教授 (40091483)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥14,880,000 (Direct Cost: ¥12,600,000、Indirect Cost: ¥2,280,000); Fiscal Year 2008: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2007: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2006: ¥5,000,000 (Direct Cost: ¥5,000,000)
• Keywords: 環境ホルモン / 魚類 / 東アジア / 生殖異常 / 国際共同調査

Research Abstract

本研究では、日中韓各国においてボラ、マハゼ、トビハゼを調査対象生物とした環境ホルモン汚染の実態調査を実施した。血中ビテロジェニン濃度、生殖腺の組織学的観察、および環境中の化学物質分析を行った結果、日本、韓国および中国の東シナ海沿岸域の大都市部周辺海域や工業・港湾地域において環境ホルモンによる汚染が確認され、魚類の繁殖現象に異常が起きていることが解った。また、本研究の目的のひとつである環境ホルモンおよび魚類繁殖研究に関する研究者ネットワークの構築も着実に進み、日中韓の大学、特に韓国済州大学と中国上海水産大学との間で、大学院生を主体としたセミナーや共同研究が継続的に行われる事となった。

Research Products

• [Journal Article] Survey of contamination of estrogenic chemicals in Japanese and Korean coastal waters using the wild grey mullet (Mugil cephalus) (2010)
  • Author(s): Aoki, J., Nagae, M., Takao, Y., Hara, A., Lee, Y. D., Yeo, I. K., Lim, B. S., Park, C. B., Soyano, K.
  • Journal Title: Science of the Total Environment 408 Pages: 660-665
  • Peer Reviewed
• [Journal Article] Gonadal development and spawning rhythm in mudskipper (Periophthalmus modestus) (2008)
  • Author(s): Soyano, K., Higuchi, T., Kobayashi, M., Takushima, M., Aoki, J., Nagae, M.
  • Journal Title: International Journal of Ichthyology (CYBIUM) 32 Pages: 234-234
  • Peer Reviewed
• [Journal Article] Survey of contamination by endocrine disrupting chemicals (EDCs) using wild grey mullet in Korea and Japan, and the cloning of estrogen receptor gene as new biomarker for effect evaluation of EDCs (2008)
  • Author(s): Aoki, J., Nagae, M, Takao, Y., Hara, A., Lee, YD, Yeo, IK., Lim, BS., Park, CB., Soyano, K.
  • Journal Title: International Journal of Ichthyology (CYBIUM) 32 Pages: 253-254
  • Peer Reviewed
• [Journal Article] Seasonal and diurnal fluctuations in the concentrations of pharmaceuticals and personal care products (PPCPs) in residential sewage water (2008)
  • Author(s): Takao, Y., Oishi, M., Nagae, M., Kohra S., Arizono, K.
  • Journal Title: Journal of Health Science 54 Pages: 240-243
  • NAID: 110006649678
  • Peer Reviewed
• [Journal Article] Gonadal development and spawning rhythm in mudskipper (Periophthalmus modestus). (2008)
  • Author(s): Sovano. K., Higuchi, T., Kobayashi, M., Takushima, M., Aoki, J., Nagae. M.
  • Journal Title: International Journal of Ichthyology (CYBIUM) 32 Pages: 234-234
  • Peer Reviewed
• [Journal Article] Survey of contamination by endocrine disrupting chemicals (EDCs) using wild gray mullet in Korea and Japan, and the cloning of estrogen receptor gene as new biomarker for effect evaluation of EDCs. (2008)
  • Author(s): Aoki, J., Nagae. M. Takao. Y., Hara, A., Lee, YD, Yeo, IK., Lim, BS., Park, CB., Soyano, K.
  • Journal Title: International Journal of Ichthyology (CYBIUM) 32 Pages: 253-254
  • Peer Reviewed
• [Journal Article] Bisphenol A incorporated into eggs from parent fish persists for several days. (2008)
  • Author(s): Takao, Y., Oishi, M., Nagae, M., Kohra S., Arizono, K.
  • Journal Title: Journal of Health Science 54 Pages: 235-239
  • NAID: 110006649677
  • Peer Reviewed
• [Journal Article] Seasonal and diurnal fluctuations in the concentrations of pharmaceuticals and personal care products (PPCPs) in residential sewage water. (2008)
  • Author(s): Takao, Y, Shimazu, M., Fukuda, M., Ishibashi, H., Nagae, M., Kohra, S., Tabira, Y., Ishibashi Y., Arizono, K.
  • Journal Title: Journal of Health Science 54 Pages: 240-243
  • NAID: 110006649678
  • Peer Reviewed
• [Journal Article] Egg yolk proteins in gray mullet (Mugil cephalus) : purification and classification of multiple lipovitellins and other vitellogenin-derived yolk proteins and molecular cloning of the parent vitellogenin genes (2007)
  • Author(s): Amano H., Fujita T., Hiramatsu N., Shimizu M., Sawaguchi S., Matsubara T., Kagawa H., Nagae M., Sullivan C.V., Hara A.
  • Journal Title: Journal of Experimental Zoology 307A Pages: 324-341
  • Peer Reviewed
• [Journal Article] Egg yolk proteins in gray mullet (Mugil cephalus): purification and classification of multiple lipovitellins and other vitellogenin-derived yolk proteins and molecular cloning of the parent vitellogenin genes. (2007)
  • Author(s): Amano H., Fujita T., Hiramatsu N., Shimizu M., Sawaguchi S., Matsubara T., Kagawa H., Nagae M., Sullivan C.V. and Hara A.
  • Journal Title: Journal of Experimental Zoology 307A Pages: 324-341
  • Peer Reviewed
• [Journal Article] Ecotoxicological effect of polycyclic musks on Caenorhabditis elegans (2006)
  • Author(s): Mori, T., Morita, F., Inokuchi, A., Takao, Y., Kohra, S., Tominaga, N., Takamasa, T., Arizono, K.
  • Journal Title: Journal of Health Science 52 Pages: 276-282
  • NAID: 110006251376
  • Peer Reviewed
• [Journal Article] 生物学的影響調査と分析化学的調査の地方フィールドにおける共有の必要性 (2006)
  • Author(s): 高雄雄二・征矢野清・長江真樹
  • Journal Title: Endocrine Disrupter News Letter 8 Pages: 5-5
  • Peer Reviewed
• [Journal Article] Ecotoxicological effect of polycyclic musks on Caenorhabditis elegans. (2006)
  • Author(s): Mori, T., Morita, F., Inokuchi, A., Takao, Y., Kohra, S., Tominaga, N., Takamasa, T., Arizono, K.
  • Journal Title: J.Health Sci. 52 Pages: 276-282
  • NAID: 110006251376
• [Journal Article] 生物学的影響調査と分析化学的調査の地方フィールドにおける共有の必要性 (2006)
  • Author(s): 高雄雄二, 征矢野清, 長江真樹
  • Journal Title: Endocrine Eisrupter News Letter 8 Pages: 5-5
• [Presentation] 多環芳香族炭化水素がボラのエストロジェン応答遺伝子の発現に及ぼす影響 (2008)
  • Author(s): 青木純哉, 征矢野清
  • Organizer: 第11回環境ホルモン学会
  • Place of Presentation: 東京ビッグサイト
  • Year and Date: 2008-12-14
• [Presentation] Effect of ethynylestradiol on development of gonad in gray mullet Mugil cephalus. (2008)
  • Author(s): Aoki, J., Hatsuyama, A., Hara, A., Sovano. K.
  • Organizer: 5th World Fisheries Congress
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 2008-10-21
• [Presentation] Effect of ethynylestradiol on development of gonad in grey mullet Mugil cephalus (2008)
  • Author(s): Aoki, J., Hatsuyama, A., Hara, A., Soyano, K.
  • Organizer: 5th World Fisheries Congress
  • Place of Presentation: Yohohama, Japan
• [Presentation] Effect of ethynylestradiol on sex differentiation in grey mullet (2008)
  • Author(s): Aoki, J., Hara, A., Soyano, K.
  • Organizer: 5th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
  • Place of Presentation: Juju, Korea
• [Presentation] Effects of temperature and salinity on reproductive activity of the mangrove killifish, Kryptolebias marmoratus (2008)
  • Author(s): Park, C.B., Lee, Y.D., Soyano, K.
  • Organizer: 5th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
  • Place of Presentation: Juju, Korea
• [Presentation] Effects of photoperiod and temperature on gonadal development of the mudskipper, Periophthalmus modestus (2008)
  • Author(s): Sobue, M., Aoki, J., Soyano, K.
  • Organizer: 5th International Meeting on Reproductive Biology of Aquatic Animals of the East China Sea
  • Place of Presentation: Juju, Korea
• [Presentation] 多環芳香族炭化水素がボラのエストロジェン応答遺伝子の発現に及ぼす影響 (2008)
  • Author(s): 青木純哉・征矢野清
  • Organizer: 第11回環境ホルモン学会
  • Place of Presentation: 東京
• [Presentation] 環境試料からの銅ピリチオンの高濃度検出 (2008)
  • Author(s): 大園明寛, 桑原和子, 阿曽沼健太郎, 李政勲, 高良真也, 長江真樹, 征矢野清, 有薗幸司, 高尾雄二
  • Organizer: 第11回環境ホルモン学会
  • Place of Presentation: 東京
• [Presentation] Effects of methyltestosterone on gametogenesis and steroidogenesis of mangrove killifish (2008)
  • Author(s): Park, C.-B., Lee, Y.-D., Soyano, K.
  • Organizer: Kryptolebias marmoratus. 第11回環境ホルモン学会
  • Place of Presentation: 東京
• [Presentation] 漁港底質中の有機スズ化合物濃度の経年変化 (2008)
  • Author(s): 桑原和子, 大園明寛, 奈須一晃, 李政勲, 柳英硯, 趙顕書, 高良真也, 長江真樹, 征矢野清, 有薗幸司, 高尾雄二
  • Organizer: 第11回環境ホルモン学会
  • Place of Presentation: 東京
• [Presentation] 千葉県及び神奈川県の漁港底質中の有機スズ化合物濃度及び他地域との比較 (2008)
  • Author(s): 大園明寛, 桑原和子, 阿曽沼健太郎, 長江真樹, 李政勲, 趙顕書, 征矢野清, 有薗幸司, 高尾雄二
  • Organizer: 第17回環境化学討論会
  • Place of Presentation: 神戸
• [Presentation] Survey of contaminations by endocrine disrupting chemicals (EDCs) using wild grey mullet in Korea and Japan, and the cloning of estrogen receptor gene as new biomarker for effect evaluation of EDCs (2007)
  • Author(s): Aoki, J., Nagae, M., Takao, Y., Hara, A., Lee, Y-D., Yeo, I-K., Lim, B-S., Park, C-B., Soyano, K.
  • Organizer: 8th International Symposium on Reproductive Physiology of Fish
  • Place of Presentation: Saint Malo, France
• [Presentation] Survey of contaminations by endocrine disrupting chemicals(EDCs) using wild grey mullet in Korea and Japan,and the cloning of estrogen receptor gene as new biomarker for effect evaluation of EDCs (2007)
  • Author(s): Aoki, J., Nagae, M., Takao, Y., Hara, A., Lee, Y-D., Yeo, I-K, Lim, B-S., Park, C-B., Soyano, K.
  • Organizer: 8^<th> International Symposium on Reproductive Physiology of Fish.
  • Place of Presentation: Saint Malo,France.
• [Book] Development of evaluation systems for the detection of estrogenic endocrine disrupting chemicals (EDCs) in aquatic environments using estrogen-inducible biomarkers(In National Taiwan Museum Special Publication 14) (2010)
  • Author(s): Hiramatsu, N., Luo, W., Hong, L., Soyano, K., Aoki, J., Amano, H., Fujita, T., Todo, T., Matsubara, T., Hara, A.
  • Publisher: National Taiwan Museum
• [Book] Coastal Environmental and Ecosystem Issues of the East China Sea. (Contaminations by endocrine disrupting chemicals in coastal waters of the East China Sea., eds. Ishimatsu, A. and Lie H.-J.) (2010)
  • Author(s): Soyano, K., Aoki, J., Itashiki, Y., Park, C. B., Nagae, M., Takao, Y., Lee, Y.-D., Yeo, I.-K., Zhong, J.
  • Publisher: Nagasaki University/TERRAPUB
• [Book] Coastal Environmental and Ecosystem Issues of the East China Sea. (Relationship between concentration of chemical substances in estuarine sediments and concentration of vitellogenin in mudskipper (Periophthalmus modestus) and common goby (Acanthogobius flavimanus) serum, eds. Ishimatsu, A. and Lie H.-J.) (2010)
  • Author(s): Takao, Y., Kuwahara, K., Nagae, M., Soyano, K.
  • Publisher: Nagasaki University/TERRAPUB, Tokyo
• [Book] Coastal Environmental and Ecosystem Issues of the East China Sea. (Estrogenic activity in estuaries by measuring serum vitellogenin concentration of Japanese mele common goby in northwestern part of Kyushu, eds. Ishimatsu, A. and Lie H.-J.) (2010)
  • Author(s): Nagae, M., Takao, Y., Ohkubo, N., Matsubara, T., Soyano, K.
  • Publisher: Nagasaki University/TERRAPUB