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Genetic analysis of drug resistant trypanosomes

Research Project

Project/Area Number 18405037
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field Applied veterinary science
Research InstitutionHokkaido University

Principal Investigator

ONUMA Misao  Hokkaido University, 名誉教授 (70109510)

Co-Investigator(Kenkyū-buntansha) OHASHI Kazuhiko  北海道大学, 大学院・獣医学研究科, 教授 (90250498)
INOUE Noboru  帯広畜産大学, 原虫病研究センター, 准教授 (10271751)
KONNAI Satoru  北海道大学, 大学院・獣医学研究科, 准教授 (40396304)
Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥15,550,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥2,250,000)
Fiscal Year 2008: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2007: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2006: ¥5,800,000 (Direct Cost: ¥5,800,000)
Keywordsトリパノソーマ / 薬剤耐性 / 抗原虫薬 / 遺伝子多型
Research Abstract

トリパノソーマ原虫は人、家畜および野生動物に感染し睡眠病、ナガナ病、スーラ病などを引き起こし、多大な被害を与えている。本症は、ワクチンによる制御が困難なうえ、近年、原虫薬剤耐性株の出現によって、その被害は深刻化している。そこで原虫遺伝子多型および薬剤耐性機序解明を目的として、ザンビア共和国およびフィリピン共和国における本症の分子疫学調査を行った。さらに、媒介昆虫であるツェツェバエの吸血宿主の同定、すなわち本症のレゼルボア動物の推定のために、トリパノソーマ感染ツェツェバエ由来のDNAサンプルに含まれる哺乳類動物のミトコンドリア遺伝子を検出し、その遺伝子配列解析から吸血宿主を同定した。その結果、ヒトおよびアフリカゾウ、アフリカバッファローなどの野生動物に加え、調査地区集落の主要な家畜であるヤギの遺伝子も検出されたことから、ヤギにおける本症の分子疫学調査も行い、同地区の高率な感染率を報告した。一方、フィリピン共和国において集団流産が認められた家畜の原因病原体の調査も実施するとともに、抗原虫薬剤治療歴を持つ家畜からトリパノソーマ原虫を分離し薬剤感受性ならびに病原性解析を行った。

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (15 results)

All 2009 2008 2007 2006

All Journal Article (11 results) (of which Peer Reviewed: 10 results) Presentation (4 results)

  • [Journal Article] Development and application of a quantitative real-time PCR for the diagnosis of Surra in water buffaloes2009

    • Author(s)
      Konnai S, Mekata H, Mingala C. N., Abes N. S., Gutierrez C. A., Herrera R. V., Dargantes A. P., Witola W. H., Cruz L. C., Inoue N., Onuma M., Ohashi K
    • Journal Title

      Infect. Genet. Evol (in press)

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Development and application of a quantitative real-time PCR for the diagnosis of Surra in water buffaloes2009

    • Author(s)
      Konnai S, Mekata H, Onuma M, et.al.
    • Journal Title

      Infect. Genet. Evol. (in press)

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] survey of abortifacient infectious agents in livestock in Luzon, the Philippines, with emphasis on the situation in a cattle herd with abortion problems2008

    • Author(s)
      Konnai S, Mingala C. N, Sato M, Abes N. S, Venturina F. A, Gutierrez C. A, Sano T, Omata Y, Cruz L. C, Onuma M, Ohashi K. A
    • Journal Title

      Acta Trop 105

      Pages: 269-273

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Detection of Trypanosoma brucei infield-captured tsetse flies and identification of host species fed on by the infected flies2008

    • Author(s)
      Konnai S, Mekata H, Odbleg R, Simmunza M, Chembensof M. Witra W. H, Tembo M. E, Chitanbo H, Inoue N, Onuma M, Ohashi K.
    • Journal Title

      Vector Borne Zoonotic Dis 8

      Pages: 565-574

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Prevalence and Source of Typanosome Infections in Field-Captured Vector Flies(Glossina pallidipes)in Southeastern Zambia2008

    • Author(s)
      Mekata H, Konnai S, Simmunza M, Chembensof M, Kano R, Witra W. H, TemboM. E, Chitanbo H, Inoue N, Onuma M, Ohashi K.
    • Journal Title

      J.Vet.Med.Sci 70

      Pages: 923-928

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] A survey of abortifacient infectious agents in livestock in Luzon, the Philippines, with emphasis on the situation in a cattle herd with abortion problems2008

    • Author(s)
      Konnai S, Mingala N, Onuma M, et.al.
    • Journal Title

      Acta Trop. 105

      Pages: 269-273

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of Trypanosoma brucei in field-captured tsetse flies and identification of host species fed on by the infected flies2008

    • Author(s)
      Konnai S, Mekata H, Onuma M, et.al.
    • Journal Title

      Vector Borne Zoonotic Dis. 8

      Pages: 565-574

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Prevalence and Source of Typanosome Infections in Field-Captured Vector Flies (Glossina pallidipes) in Southeastern Zambia2008

    • Author(s)
      Mekata H, Konnai S, Ohashi K, et.al.
    • Journal Title

      J. Vet. Med. Sci. 70

      Pages: 923-928

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A survey of abortifacient infectious agents in livestock in Luzon, the Philippines, with emphasis on the situation in a cattle herd with abortion problems2008

    • Author(s)
      Konnai S, Mingala N, Ohashi K, et. al.
    • Journal Title

      Acta Tropica 105

      Pages: 269-273

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Detection of Trypanosoma brucei in field-captured tsetse flies and identification of host species fed on by the infected flies2008

    • Author(s)
      Konnai S, Mekata H, Ohashi K, et. al.
    • Journal Title

      Vector Borne and Zoonotic Diseases (in press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The effect of TAO expression on PCD-like phenomenon development and drug resistance in Trypanosoma brucei.2006

    • Author(s)
      Tsuda A, Witola WH, Konnai S, Ohashi K, Onuma M
    • Journal Title

      Parasitol. Int. 55

      Pages: 135-142

    • NAID

      10018192565

    • Related Report
      2006 Annual Research Report
  • [Presentation] ペルー共和国およびボレリア共和国における牛トリパノソーマ病の分子疫学調査2008

    • Author(s)
      目堅博久, 今内覚, 差上晃, 小沼操, 太橋租彦
    • Organizer
      第146回日本獣医学会学術集会
    • Place of Presentation
      宮崎県宮崎市(シーガイヤ)
    • Related Report
      2008 Annual Research Report 2008 Final Research Report
  • [Presentation] ザンビア共和国におけるトリパノソーマ症の疫学調査とその伝播経路の探索2007

    • Author(s)
      目堅博久, 今内覚, R. Odbileg, M. Simuunza, M. Chembensofu, W. Witola, M. Tembo, H. Chitambo, 井上昇, 小沼操, 大橋和彦
    • Organizer
      第144回日本獣医学会学術集会
    • Place of Presentation
      北海道江別市(酪農学園大学)
    • Related Report
      2008 Final Research Report 2007 Annual Research Report
  • [Presentation] フィリピン共和国におけるTrypanosomaevansiの分子疫学調査2007

    • Author(s)
      今内覚, 目堅博久, C. N. Mingala, N. S. Abes, C. A. Gutierrez, R. herrera, W. H. Witola, L. C. Cruz, 井上昇, 小沼操, 大橋和彦
    • Organizer
      第144回日本獣医学会学術集会
    • Place of Presentation
      北海道江別市(酪農学園大学)
    • Related Report
      2008 Final Research Report
  • [Presentation] フィリピン共和国におけるTrypanosoma evansiの分子疫学調査2007

    • Author(s)
      今内 覚, 目堅博久, 小沼 操, 他.
    • Organizer
      第144回日本獣医学会学術集会
    • Place of Presentation
      北海道江別市(酪農学園大学)
    • Related Report
      2007 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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