Survey of contamination of enteropathogenic bacteria in Myanmar

• Project/Area Number: 18406014
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Bacteriology (including Mycology)
• Research Institution: Okayama University
• Principal Investigator: OKAMOTO Keinosuke 岡山大, 医歯(薬)学総合研究科, 教授 (70131183)
• Co-Investigator(Kenkyū-buntansha): 三好 伸一 岡山大学, 大学院・医歯薬学総合研究科, 教授 (60182060) ; 中尾 浩史 岡山大学, 大学院・医歯薬学総合研究科, 准教授 (20237217) ; 高橋 栄造 岡山大学, 大学院・医歯薬学総合研究科, 助教 (70379733)
• Project Period (FY): 2006 – 2009
• Project Status: Completed (Fiscal Year 2009)
• Budget Amount: ¥15,910,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥2,610,000); Fiscal Year 2009: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000); Fiscal Year 2008: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2007: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2006: ¥4,600,000 (Direct Cost: ¥4,600,000)
• Keywords: 腸管感染菌 / 下痢症 / ミャンマー / 薬剤耐性 / 大腸菌 / 国際研究者交流 / 細菌 / 下痢 / 薬剤感受性 / 腸管感染症 / コレラ / アエロモナス / 抗生物質 / 病原因子 / 腸管病原性大腸菌 / 毒素原性大腸菌 / エンテロトキシン / コロニーハイブリダイゼーション

Research Abstract

ミャンマーは閉鎖的な国であり、国際間の研究交流も少ない。同国の経済状態、地理的条件、衛生状態から判断すると、多くの腸管感染症患者が発生していると考えられる。しかし海外からの細菌調査も行われていなく、実体は不明である。岡山大学はミャンマーの国立研究所であるDepartment of MedicalResearch (Lower Myanmar)(DMR 研究所)と協定を結びことができ、またマンダレー医科大学との交流もある。この研究交流を拠点にミャンマーでの腸管感染菌による患者の発生を調査し、汚染状況を明らかにする事を計画した。ミャンマー国の検査体制から、ヒトに対する汚染は大腸菌を調査対象とした。また自然界の汚染としてはビブリオとアエロモナスを対象にして検査を行う事を計画した。

Research Products

• [Journal Article] MacAB is involved in the secretion of Escherichia coli heat-stable enterotoxin II (2008)
  • Author(s): Hiroyasu Yamanaka, Hidetomo Kobayashi, Eizo Takahashi, Keinosuke Okmaoto
  • Journal Title: J. Bacterriol. 190 Pages: 7693-7698
  • Peer Reviewed
• [Journal Article] Toxin production by Aeromonas sobria in natural environment : Riverwater vs. Seawater (2008)
  • Author(s): Rasel Khan, Eizo Takahashia, HironoriNakura, Mohammad Ansaruzzaman, SukalyaniBanik, Thandavarayan Ramamurthy and Keinosuke Okamoto
  • Journal Title: Acta Medica Okayama 62 Pages: 363-371
  • Peer Reviewed
• [Journal Article] Studies on the diarrheagenic Escherichia coli isolated from children with diarrhea in Myanmar (2008)
  • Author(s): Eizo Takahashi, Zafar Sultan, Sumika Shimada, Wah Wah Aung, Mar Mar Nyein, Khin Nwe Oo, Gopinath Balakrish Nair, Yoshifumi Takeda and Keinosuke Okamoto
  • Journal Title: Microbiology and Immunology 52 Pages: 2-8
  • NAID: 10025690925
  • Peer Reviewed
• [Journal Article] Studies on the diarrheagenic Escherichia coli Isolated from children with diarrhea in Myanmar (2008)
  • Author(s): Eizo Takahashi
  • Journal Title: Microbiology and Immunology 52 Pages: 2-8
  • NAID: 10025690925
  • Peer Reviewed
• [Journal Article] Impaired plasma clottability induction through fibrinogen degradation by ASP, a serine protease released from Aeromonas sobria (2008)
  • Author(s): Takahisa Imamura
  • Journal Title: FEMS Microbiol Lett 284 Pages: 35-42
  • Peer Reviewed
• [Journal Article] Isolation of tetracycline-resistant clinical Helicobacter pylori without mutations in 16SrRNA gene in Bangladesh (2008)
  • Author(s): Rasel Khan
  • Journal Title: Microbiology and Immunology 52 Pages: 508-511
  • Peer Reviewed
• [Journal Article] MacAB is involved in the secretion of Escherichia coli heat-stable enterotoxin II (2008)
  • Author(s): Hiroyasu Yamanaka
  • Journal Title: J. Bacterriol. 190 Pages: 7693-7698
  • Peer Reviewed
• [Journal Article] Toxin Production by Aeromonas sobria in Natural Environment River Water vs. Seawater (2008)
  • Author(s): Rasel Khan
  • Journal Title: Acta Medica Okayama 62 Pages: 363-371
  • Peer Reviewed
• [Journal Article] Fluid secretion caused by aerolysin-like hemolysin of Aeromonas sobria in the intestines is due to stimulation of production of prostaglandin E2 viacyclooxygenase-2 by the intestinal cells. (2008)
  • Author(s): Fujii Yoshio
  • Journal Title: Infection and Immunity 76 Pages: 1076-1082
  • Peer Reviewed
• [Journal Article] Studies on the diarrheagenic Escherichia coli isolated from children with diarrhea in Myanmar (2008)
  • Author(s): Eizo Takahashi
  • Journal Title: Microbiology and Immunology 52 Pages: 1-7
  • NAID: 10025690925
  • Peer Reviewed
• [Journal Article] Salt in surroundings influences the production of serine protease into milieu by Aeromonas sobria (2007)
  • Author(s): Rasel Khan
  • Journal Title: Microbiology and Immunology 51 Pages: 963-976
  • Peer Reviewed
• [Journal Article] Studies on the region involved in the transport activity of Escherichia coli TolC by chemeric protein analysis (2007)
  • Author(s): Hiroyasu Yamanaka,
  • Journal Title: Microbial Pathogenesis 42 Pages: 184-192
  • Peer Reviewed
• [Journal Article] Activation of prothrombin by ASP, a serine protease released from Aeromonas sobria (2007)
  • Author(s): Hidetoshi Nitta
  • Journal Title: FEBS Letters 581 Pages: 5935-5939
  • Peer Reviewed
• [Journal Article] Inhibitory effects of beer on mutation in the Ames test and DNA adduct formation in mouse organs induced by 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine(PhIP). (2006)
  • Author(s): 岡本敬の介 他
  • Journal Title: Biol.Pharm.Bull. 29・1 Pages: 67-70
  • NAID: 110005602085
• [Journal Article] Involvement of the drug efflux protein TolC in mutagenicity induced by MNNG or Trp-P-2 (2006)
  • Author(s): 岡本敬の介 他
  • Journal Title: Mutation Research-GTEM 605 Pages: 42-50
• [Journal Article] Cleavage specificity of the serine protease of Aeromonas Sobria, a member of the kexin family of subtiases (2006)
  • Author(s): 岡本敬の介 他
  • Journal Title: FEMS Microbiology Letters 256・1 Pages: 165-170
• [Journal Article] Aeromonas sobria hemolysin causes diarrhea by increasing secretion of HCO3- (2006)
  • Author(s): 岡本敬の介 他
  • Journal Title: FEMS Microbiology Letters 258 Pages: 92-95
• [Journal Article] Induction of vascular leakage and blood pressure lowering through kinin release by a seine protease from Aeromonas (2006)
  • Author(s): 岡本敬の介 他
  • Journal Title: J.Immunol. 177 Pages: 8723-8729
• [Presentation] 腸炎ビブリオの産生するシデロフォア, vibrioferrin と病原性 (2009)
  • Author(s): 山田真人, 高橋栄造, 岡本敬の介, 田邊知孝, 山本重雄, 中尾浩史
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都
  • Year and Date: 2009-03-27
• [Presentation] 腸炎ビブリオの産生するシデロフォア, vibrioferrinと病原性 (2009)
  • Author(s): 山田真人
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都
  • Year and Date: 2009-03-27
• [Presentation] アエロモナスの菌体外毒素産生に及ぼす食塩の影響 (2009)
  • Author(s): 高橋栄造
  • Organizer: 日本薬学会第129年会
  • Place of Presentation: 京都
  • Year and Date: 2009-03-27
• [Presentation] 食塩によるアエロモナスの菌体外リパーゼの産生制御 (2009)
  • Author(s): 高橋栄造
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-13
• [Presentation] 毒素原性大腸菌が産生する耐熱性エンテロトキシン(ST)の分泌過程の解析 (2009)
  • Author(s): 山中浩泰
  • Organizer: 第82回日本細菌学会総会
  • Place of Presentation: 名古屋
  • Year and Date: 2009-03-13
• [Presentation] Effect of Salt on the Production of Exotoxins by Aeromonas sobria (2008)
  • Author(s): E. Takahashi
  • Organizer: Asia-African Research Forum on Emerging and Reemerging Infections
  • Place of Presentation: 札幌
  • Year and Date: 2008-12-15
• [Presentation] Factors of Aeromonas involved in sepsis (2008)
  • Author(s): Eizo Takahashi , Keinosuke Okamoto
  • Organizer: Joint Conference on Cholera and other bacterial enteric infections panel
  • Place of Presentation: 福岡
  • Year and Date: 2008-11-17
• [Presentation] Aeromonas sobriaの菌体外毒素リパーゼの食塩による産生抑制 (2008)
  • Author(s): 高橋栄造
  • Organizer: 第61回日本細菌学会中国・四国支部総会
  • Place of Presentation: 松山
  • Year and Date: 2008-10-18
• [Presentation] 生合成過程での研究例-アエロモナスの菌体外毒素の生合成および成熟化過程 (2008)
  • Author(s): 岡本敬の介
  • Organizer: 第20回微生物シンポジウム
  • Place of Presentation: 岐阜
  • Year and Date: 2008-09-03
• [Presentation] Aeromonas sobriaの産生するセリンプロテアーゼの成熟化機構の解析 (2008)
  • Author(s): 小林秀丈
  • Organizer: 第55回毒素シンポジウム
  • Place of Presentation: 山梨県
  • Year and Date: 2008-07-02
• [Presentation] Aeromonas sobria の生息環境に関する研究 (2008)
  • Author(s): 高橋栄造
  • Organizer: 第55回毒素シンポジウム
  • Place of Presentation: 山梨県
  • Year and Date: 2008-07-02
• [Presentation] Influence of salt surroundings on the production of proteases into milieu (2007)
  • Author(s): Eizo Takahashi
  • Organizer: Joint Conference on Cholera and other bacterial enteric infections pan
  • Place of Presentation: Austin, Texas, USA
  • Year and Date: 2007-12-06
• [Presentation] アエロモナスの致死活性因子の解析 (2007)
  • Author(s): 田村謙吾
  • Organizer: 第46回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会
  • Place of Presentation: 高知市
  • Year and Date: 2007-11-10
• [Presentation] Aeromonasのプロテアーゼの産生と生存 (2007)
  • Author(s): 小野裕子
  • Organizer: 第46回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会
  • Place of Presentation: 高知市
  • Year and Date: 2007-11-10
• [Presentation] Production and Maturation pathway of exotoxins of enteropathogenic bacteria (2007)
  • Author(s): Keinosuke Okamoto
  • Organizer: Myanmar Health Research Congress 2006
  • Place of Presentation: Yangon, Myanmar
  • Year and Date: 2007-01-10
• [Presentation] Aeromons sobriaのメタロプロテアーゼの産生に関する解析 (2007)
  • Author(s): 高橋栄造
  • Organizer: 第54回毒素シンポジウム
  • Place of Presentation: 大阪府泉佐野市
• [Presentation] Aeromons sobriaの産生するセリンプロテアーゼに関する研究 (2007)
  • Author(s): 小林秀丈
  • Organizer: 第54回毒素シンポジウム
  • Place of Presentation: 大阪府泉佐野市
• [Presentation] Aeromons sobriaのメタロプロテアーゼの産生に関する解析 (2007)
  • Author(s): 高橋栄造
  • Organizer: 第60回日本細菌学会中国・四国支部総会
  • Place of Presentation: 岡山県総社市
• [Presentation] Tn10 mutation to reduce the production of serine protease of Aeromonas sobria (2007)
  • Author(s): Rasel Khan
  • Organizer: 第60回日本細菌学会中国・四国支部総会
  • Place of Presentation: 岡山県総社市
• [Presentation] 大腸菌耐熱性エンテロトキシン(ST)の菌体外分泌過程の解析 (2007)
  • Author(s): 山中浩泰
  • Organizer: 第60回日本細菌学会中国・四国支部総会
  • Place of Presentation: 岡山県総社市
• [Presentation] Crystal structure of Aeromonas sobria serine protease, prototype Kexin/Furin protease: The extra occluding domain regulates protein substrate specificity (2007)
  • Author(s): Hideaki Tsuge
  • Organizer: 5th General Meeting of the International Proteolysis Society (IPS2007)
  • Place of Presentation: Patras, Greece
• [Presentation] Vibrio fluvialis: an emerging pathogen in causing acute diarrhea in Kolkata, India (2007)
  • Author(s): Gautom Chowdhuray,
  • Organizer: Vibrio 2007
  • Place of Presentation: Institute Pasteur, Paris, France
• [Presentation] 環境中のアエロモナスと病原性 (2006)
  • Author(s): 李云善、高橋栄造、岡本敬の介
  • Organizer: 第5回ミャンマーを起点とするアジア医療拠点シンポジウム
  • Place of Presentation: 岡山
  • Year and Date: 2006-06-12
• [Book] 微生物学用語集 (2007)
  • Author(s): 岡本敬の介, 他7名
  • Total Pages: 519
  • Publisher: 南山堂
• [Book] 微生物学用語集 (2007)
  • Author(s): 日本細菌学会用語委員会, 櫻井
  • Total Pages: 519
  • Publisher: 南山堂
• [Patent(Industrial Property Rights)] スルホンアミド基を有する抗菌剤 (2007)
  • Inventor(s): 加来田、岡本、他
  • Industrial Property Rights Holder: 岡山大学
  • Filing Date: 2007-11-08