Study of characteristics and prevalence of novel species rotavirus detected in Asia

• Project/Area Number: 18406018
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 海外学術
• Research Field: Virology
• Research Institution: Sapporo Medical University
• Principal Investigator: KOBAYASHI Nobumichi Sapporo Medical University, 医学部, 教授 (80186759)
• Co-Investigator(Kenkyū-buntansha): ISHINO Masaho 札幌医科大学, 医学部, 准教授 (30232325) ; SUMI Ayako 札幌医科大学, 医学部, 講師 (10363699) ; NAGASHIMA Shigeo 札幌医科大学, 医学部, 助教 (60433116) ; MISE Keiji 札幌医科大学, 医学部, 助手 (30200025) ; TANIGUCHI Koki 藤田保健衛生大学, 医学部, 教授 (40094213)
• Research Collaborator: T.N Naik インド国立コレラ腸管感染症研究所 ; T Krishnan インド国立コレラ腸管感染症研究所 ; M Chawla-sarkar インド国立コレラ腸管感染症研究所 ; S.K Paul マイメンシン医科大学 ; M.M Alam バングラデシュ農科大学 ; T.S Aung ミャンマー国立保健研究所 ; Y-H Wang 武漢市CDC ; X Zhou 武漢市CDC
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥15,750,000 (Direct Cost: ¥13,500,000、Indirect Cost: ¥2,250,000); Fiscal Year 2008: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2007: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2006: ¥6,000,000 (Direct Cost: ¥6,000,000)
• Keywords: ロタウイルス / 新種 / 成人 / 下痢症 / 分子疫学 / アジア / 新興型 / B 群 / B群 / 熱帯地域

Research Abstract

バングラデシュで成人下痢症より検出された非定型的ヒトロタウイルスB219株の全遺伝子配列を決定し、遺伝子解析を行った結果、B219株は従来から知られるA群、B群、C群ロタウイルスとは遺伝学的に異なる新種(群)に属することが判明した。アジアでの成人・小児由来ロタウイルスの疫学調査では新種ロタウイルスは検出されず浸淫度は低いと考えられたが、A群ロタウイルスは小児と成人の間で感染・伝播していることが示唆された。

Research Products

• [Journal Article] Full genomic analysis of a human group A rotavirus G9P[6] strain from Eastern India provides evidence for porcine to human interspecies transmission (2009)
  • Author(s): Mukherjee A, Dutta D, Ghosh S, Bagchi P, Chattopadhyay S, Nagashima S, Kobayashi N, Dutta P, Krishnan T, Naik TN, Chawla-Sarkar M.
  • Journal Title: Archives of Virology (印刷中)
  • Peer Reviewed
• [Journal Article] Phylogenetic analysis of rotaviruses with predominant G3 and emerging G9 genotypes from adults and children in Wuhan, China (2009)
  • Author(s): Wang Y-H, Kobayashi N, Zhou X, Nagashima S, Zhu Z-R Peng J-S, Liu M-Q, Hu Q, Zhou D-J, Watanabe S, Ishino M.
  • Journal Title: Journal of Medical Virology 81 Pages: 382-389
  • Peer Reviewed
• [Journal Article] Phylogenetic analysis of rotaviruses with genotypes G1, G2, G9 and G12 in Bangladesh : evidence for a close relationship between rotaviruses from children and adults (2008)
  • Author(s): Paul SK, Kobayashi N, Nagashima S, Ishino M, Watanabe S, Alam MM, Ahmed MU, Hossain MA, Naik TN.
  • Journal Title: Archives of Virology 153 Pages: 1999-2012
  • Peer Reviewed
• [Journal Article] Human group A rotavirus P[8] Hun9-like and rare OP354-like strains are circulating among diarrhoeic children in Eastern India (2008)
  • Author(s): Samajdar S, Ghosh S, Dutta D, Chawla-Sarkar M, Kobayashi N, Naik TN.
  • Journal Title: Archives of Virology 153 Pages: 1933-1936
  • Peer Reviewed
• [Journal Article] Increase in prevalence of human group A rotavirus G9 strains as an important VP7 genotype among children in eastern India (2008)
  • Author(s): Samajdar S, Ghosh S, Chawla-Sarkar M, Mitra U, Dutta P, Kobayashi N, Naik TN.
  • Journal Title: J Clin Virol 43 Pages: 334-339
  • Peer Reviewed
• [Journal Article] Whole genomic characterization of a human rotavirus strain B219 belonging to a novel group of the genus Rotavirus (2008)
  • Author(s): Nagashima S, Kobayashi N, Ishino M, Alam MM, Ahmed MU, Paul SK, Ganesh B, Chawla-Sarkar M, Krishnan T, Naik TN, Wang Y-H.
  • Journal Title: J Med Virol 80 Pages: 2023-2033
  • Peer Reviewed
• [Journal Article] Identical rearrangement of NSP3 genes found in three independently isolated virus clones derived from mixed infection and multiple passages of rotaviruses (2008)
  • Author(s): Alam MM, Kobayashi N, Ishino M, Nagashima S, Paul SK, Chawla-Sarkar M, Krishnan T, Naik TN.
  • Journal Title: Arch Virol 153 Pages: 555-559
  • Peer Reviewed
• [Journal Article] Whole genomic characterization of a human rotavirus strain B219 belonging to a novel group of the genus Rotavirus. (2008)
  • Author(s): Nagashima S, et al.
  • Journal Title: Journal of Medical Virology 80 Pages: 2023-2033
  • Peer Reviewed
• [Journal Article] Phylogenetic analysis of rotaviruses with genotypes G1, G2, G9 and G12 in Bangladesh : evidence for a close relationship between rotaviruses from children and adults. (2008)
  • Author(s): Paul SK, et al.
  • Journal Title: Archives of Virology 153 Pages: 1999-2012
  • Peer Reviewed
• [Journal Article] Phylogenetic analysis of rotaviruses with predominant G3 and emerging G9 genotypes from adults and children in Wuhan, China. (2008)
  • Author(s): Wang Y, et al.
  • Journal Title: Journal of Medical Virology 81 Pages: 382-389
  • Peer Reviewed
• [Journal Article] Identical rearrangement of NSP3 genes found in three independently isolated virus clones derived from mixed infection and multiple passages of rotaviruses. (2008)
  • Author(s): Alam M, et. al.
  • Journal Title: Archives of Virology 153 Pages: 555-559
  • Peer Reviewed
• [Journal Article] Evidence for bovine origin of VP4 and VP7 genes of human group A rotavirus G6P[14] and G10P[14] strains (2007)
  • Author(s): Ghosh S, Varghese V, Samajdar S, Sinha M, Naik TN, Kobayashi N.
  • Journal Title: J Clin Microbiol 45 Pages: 2751-2753
  • Peer Reviewed
• [Journal Article] Study of the effect of vaccination on periodic structures of measles epidemics in Japan (2007)
  • Author(s): Sumi A, Kamo K, Ohtomo N, Kobayashi N.
  • Journal Title: Microbiol Immunol 51 Pages: 805-814
  • NAID: 10020279045
  • Peer Reviewed
• [Journal Article] Molecular epidemiologic analysis of group A rotaviruses in adults and children with diarrhea in Wuhan city, China, 2000-2006 (2007)
  • Author(s): Wang Y-H, Kobayashi N, Zhou D-Z, Yang Z-Q, Zhou X, Peng J-S, Zhu Z-R, Zhao D-F, Liu M-Q, Gong J.
  • Journal Title: Arch Virol 152 Pages: 669-685
  • Peer Reviewed
• [Journal Article] Genetic analysis of an ADRV-N-like rotavirus strain B219 detected in a sporadic case of adult diarrhea in Bangladesh (2007)
  • Author(s): Alam MM, Kobayashi N, Ishino M, Ahmed MS, Ahmed MU, Paul SK, Muzumdar BK, Hussain Z, Wang Y-H, Naik TN.
  • Journal Title: Arch Virol 152 Pages: 199-208
  • Peer Reviewed
• [Journal Article] Molecular epidemiologic analysis of group A rotaviruses in adults and children with diarrhea in Wuhan city, China,2000-2006. (2007)
  • Author(s): Wang YH, et. al.
  • Journal Title: Archives of Virology 152 Pages: 669-685
  • Peer Reviewed
• [Journal Article] Evidence for bovine origin of VP4 and VP7 genes of human group A rotavirus G6P [14] and G10P[14] strains. (2007)
  • Author(s): Ghosh S, et. al.
  • Journal Title: Journal of Clinical Microbiology 45 Pages: 2751-2753
  • Peer Reviewed
• [Journal Article] Comparison of NSP4 protein between group A and B human rotaviruses : detection of novel diarrhea-causing sequence in group BNSP4. (2006)
  • Author(s): Ishino M, et al.
  • Journal Title: Arch Virol 151 Pages: 173-182
• [Journal Article] An outbreak of rotavirus infection among adults in an institution for rehabilitation : long-term residence in a closed community as a risk factor for rotavirus illness. (2006)
  • Author(s): Iijima Y, et al.
  • Journal Title: Scand J Infect Dis 38 Pages: 490-496
• [Journal Article] Characterization of VP1, VP2 and VP3 gene segments of a human rotavirus closely related to porcine strains. (2006)
  • Author(s): Varghese V, et al.
  • Journal Title: Virus Genes 32 Pages: 241-247
• [Journal Article] Changing pattern of human group A rotaviruses : Emergence of G12 as an important pathogen among children in eastern India. (2006)
  • Author(s): Samajdar S, et al.
  • Journal Title: J Clin Virol 36 Pages: 183-188
• [Journal Article] RT-PCR based diagnosis revealed importance of human group B rotavirus infection in childhood diarrhoea. (2006)
  • Author(s): Barman P, et al.
  • Journal Title: J Clin Virol 36 Pages: 222-227
• [Journal Article] Analysis on genetic factors related topreferential selection of NSP1 gene segment observed in mixed infection and multiple passage of rotaviruses. (2006)
  • Author(s): Alam MM, et al.
  • Journal Title: Arch Virol 151 Pages: 2149-2159
• [Presentation] 下利便におけるプロテオーム解析 (2009)
  • Author(s): 石埜正穂、長嶋茂雄、三瀬敬治、小林宣道
  • Organizer: 第79回日本衛生学会総会
  • Place of Presentation: 東京
  • Year and Date: 2009-03-31
• [Presentation] Phylogenetic relatedness of G1, G3, and G9 rotaviruses between children and adults in Wuhan, China (2009)
  • Author(s): Kobayashi N, Wang Y-H, Zhou X, Nagashima S, Peng J-S, Zhu Z-R, Liu M-Q, Hu Q, Zhou D-J.
  • Organizer: 8th Asia Pacific Congress of Medical Virology
  • Place of Presentation: 香港
  • Year and Date: 2009-02-28
• [Presentation] Molecular epidemiologic study of norovirus in sporadic cases of gastroenteritis in all age groups in Wuhan, China, 2008 (2009)
  • Author(s): Wang Y-H, Kobayashi N, Peng J-S, Nagashima S, Zhou X, Yang T, Hu Q, Zhou D-J.
  • Organizer: 8th Asia Pacific Congress of Medical Virology
  • Place of Presentation: 香港
  • Year and Date: 2009-02-26
• [Presentation] Phylogenetic analysis of G4P[6] rotavirus strain R479 isolated in Wuhan, China (2009)
  • Author(s): Kobayashi N, Wang Y-H, Peng J-S, Nagashima S, Zhou X, Hu Q, Zhou D-J.
  • Organizer: 8th Asia Pacific Congress of Medical Virology
  • Place of Presentation: 香港
  • Year and Date: 2009-02-26
• [Presentation] "Host cellular proteins" : key determinants of rotavirus infection and pathogenesis (2008)
  • Author(s): Chawla-Sarkar M, Dutta D, Bagchi P, Krishnan T, Nagashima S, Kobayashi N.
  • Organizer: Asian-African Research Forum on Emerging and Reemerging Infections
  • Place of Presentation: 札幌
  • Year and Date: 2008-12-16
• [Presentation] バングラデシュにおける小児および成人下痢症由来A群ロタウイルスの遺伝子学的解析 (2008)
  • Author(s): 長嶋茂雄、Paul Shyamal, 渡辺祥二郎、石埜正穂、小林宣道
  • Organizer: 第56回日本ウイルス学会
  • Place of Presentation: 岡山
  • Year and Date: 2008-10-28
• [Presentation] バングラデシュにおける小児および成人下痢症由来A群ロタウイルスの遺伝子学的解析. (2008)
  • Author(s): 長嶋茂雄
  • Organizer: 日本ウイルス学会
  • Place of Presentation: 岡山市
  • Year and Date: 2008-10-28
• [Presentation] 中国・武漢市における成人および小児下痢症由来G1, G3, G9 ロタウイルスの分子疫学的解析 (2008)
  • Author(s): 小林宣道、長嶋茂雄、石埜正穂、渡辺祥二郎、Wang Yuanhong, Zhou Xuan
  • Organizer: 第56回日本ウイルス学会
  • Place of Presentation: 岡山
  • Year and Date: 2008-10-27
• [Presentation] 中国・武漢市における成人および小児下痢症由来G1, G3, G9ロタウイルスの分子疫学的解析. (2008)
  • Author(s): 小林宣道
  • Organizer: 第56回日本ウイルス学会学術集会
  • Place of Presentation: 岡山市
  • Year and Date: 2008-10-27
• [Presentation] Molecular epidemiologic analysis of group A rotaviruses in children with diarrhea in Wuhan city, China, 2007 (2008)
  • Author(s): Zhou X, Kobayashi N, Wang YH, Zhu ZR, Zhou DJ, Sun SH
  • Organizer: The 13th International Congress on Infectious Diseases
  • Place of Presentation: Kuala Lumpur, Malaysia
  • Year and Date: 2008-06-21
• [Presentation] Whole sequence analysis of novel group rotavirus B219 and group B rotaviruses (2008)
  • Author(s): Kobayashi N, Nagashima S, Alam M, Wang Y-H, Krishnan T, Chawla-Sarkar M.
  • Organizer: The 7th Japan-China International Conference of Virology
  • Place of Presentation: 東京
  • Year and Date: 2008-06-02
• [Presentation] Molecular epidemiology of rotaviruses in Wuhan, China, Jun. 2006-Feb. 2008 : predominance of variant G3 strains and increase of G9 strains (2008)
  • Author(s): Wang Y-H, Kobayashi N, Zhou X, Nagashima, S, Zhu Z-R, Sun S-H, Liu M-Q, Peng J-S, Hu Q, Zhou D-J.
  • Organizer: The 7th Japan-China International Conference of Virology
  • Place of Presentation: 東京
  • Year and Date: 2008-06-02
• [Presentation] Phylogenetic analysis of predominant G2, emerging G9 and G12 rotaviruses from children and adults in Bangladesh (2008)
  • Author(s): Paul SK, Nagashima S, Kobayashi N, Ishino M, Watanabe S, Alam MM, Ahmed MU.
  • Organizer: The 42nd Joint Working Conference on Viral Diseases, The U.S.-Japan Cooperative Medical Science Program
  • Place of Presentation: 長崎
  • Year and Date: 2008-05-27
• [Presentation] バングラデシュ(マイメンシン市)における小児および成人下痢症由来A群ロタウイルスの分子疫学的解析 (2008)
  • Author(s): 長嶋茂雄、渡辺祥二郎、石埜正穂、小林宣道
  • Organizer: 第78回日本衛生学会
  • Place of Presentation: 熊本
  • Year and Date: 2008-03-29
• [Presentation] バングラデシュ(マイメンシン市)における小児および成人下痢症由来A群ロタウイルスの分子疫学的解析. (2008)
  • Author(s): 長嶋茂雄
  • Organizer: 第78回日本衛生学会
  • Place of Presentation: 熊本市民会館
  • Year and Date: 2008-03-29
• [Presentation] B群ヒトロタウイルスのVP1およびVP3遺伝子配列の解析 (2007)
  • Author(s): 長嶋茂雄、石埜正穂、小林宣道
  • Organizer: 第55回日本ウイルス学会
  • Place of Presentation: 札幌
  • Year and Date: 2007-10-22
• [Presentation] Whole genome characterization of a human rotavirus strain B219 that belongs to a novel rotavirus group (2007)
  • Author(s): Nagashima S, Ishino M, Kobayashi N, Alam MM, Ahmed MU.
  • Organizer: The 41st Joint Working Conference on Viral Diseases, The U.S.-Japan Cooperative Medical Science Program
  • Place of Presentation: Baltimore, USA
  • Year and Date: 2007-07-24
• [Presentation] 成人下痢症由来の新奇なヒトロタウイルスB219 株の分子疫学的解析 (2007)
  • Author(s): 長嶋茂雄、石埜正穂、小林宣道
  • Organizer: 第77回日本衛生学会
  • Place of Presentation: 大阪
  • Year and Date: 2007-03-28
• [Presentation] 中国・武漢市における成人・小児下痢症由来A 群ロタウイルスの分子疫学的解析 (2007)
  • Author(s): 小林宣道、石埜正穂、長嶋茂雄、三瀬敬治、鷲見紋子
  • Organizer: 第77回日本衛生学会
  • Place of Presentation: 大阪
  • Year and Date: 2007-03-28
• [Presentation] 中国・武漢市における成人・小児下痢症由来A 群ロタウイルスの分子疫学的解析 (2006)
  • Author(s): 小林宣道、石埜正穂、長嶋茂雄、三瀬敬治、鷲見紋子
  • Organizer: 第54回日本ウイルス学会
  • Place of Presentation: 名古屋
  • Year and Date: 2006-11-20
• [Presentation] 成人下痢症由来の新種ヒトロタウイルスB219 株の遺伝子解析 (2006)
  • Author(s): 長嶋茂雄、石埜正穂、小林宣道
  • Organizer: 第54回日本ウイルス学会
  • Place of Presentation: 名古屋
  • Year and Date: 2006-11-09
• [Presentation] Genetic and epidemiologic characterization of group A rotavirus among adults and children with diarrhea in Wuhan, China, Dec. 2000-Mar. 2006 (2006)
  • Author(s): Wang Y-H, Kobayashi N, Zhou D-J, Yang Z-Q, Zhu Z-R, Zhou X, Liu M-Q, Peng J-S, Li Y.
  • Organizer: The 6th China-Japan International Conference of Virology
  • Place of Presentation: 上海
  • Year and Date: 2006-06-23
• [Presentation] Genetic analysis of an ADRV-N-like novel human rotavirus detected in a sporadic case of adult diarrhea in Bangladesh (2006)
  • Author(s): Kobayashi N, Wang Y-H, Ishino M, Alam MM, Ahmed MU.
  • Organizer: The 6th China-Japan International Conference of Virology
  • Place of Presentation: 上海
  • Year and Date: 2006-06-23
• [Book] Diversity of G-type and P-type of human and animal rotaviruses and its genetic background. In A. Mendez-Vilas, Editor (2007)
  • Author(s): Kobayashi N, Ishino M, Wang Y-H., Chawla-Sarkar M, Krishnan T, Naik T.N.
  • Publisher: Communicating Current Research and Educational Topics and Trends in Applied Microbiology (Microbiology Book Series-2007 Edition) Formatex, Spain