Structural diversity of chondroitin sulfate glycosaminoglycans and retinal pathway formation
| Project/Area Number |
18500234
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | University of Tsukuba |
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Principal Investigator |
ICHIJO Hiroyuki University of Tsukuba, Graduate School of Comprehensive Human Sciences, associate professor (40272190)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,930,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥330,000)
Fiscal Year 2007: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2006: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | retina / ganglion cell / retinotectal / optic tracxt / axon / growth cone / chondroitin sulfate / pathway formation / 網膜 / chronological sorting |
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| Research Abstract |
Chondroitin sulfate glycosaminoglycans (CSs) inhibit axonal growth of retinal ganglion cells to keep territory of their growth on the diencephalon, the optic tract. CSs' chains contains higher structural diversities; however, it is not well known function of their diversity. We prepared polystyrene beads with CSs-phosphatydyl ethanolamine derivatives adhered on their surface, and have developed assay for growth cones behaviors encountering the CSs beads on time-lapse movies; the assay enables us to examine effects of CSs and their structural diversity on the growth cone behaviors. Growth cones behaviors of the retinal axons were classified against CSs' beads in three groups: (1) no change, (2) avoidance turning, and (3) retraction. Percentages of growth cone "retraction" differ between CS-E and CS-A beads, indicating that the retinal growth cones distinguish between the beads by CSs' structural diversity. Presently, we have been confirming the results; it is expecting to examine relationship between structural diversity of CSs and its functional specificity on growth cones' behavior of the retinal axons.
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Report
(3 results)
Research Products
(8 results)
