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Analysis of brain disfunction and behavioral abnormality using the dementia or depression model mice

Research Project

Project/Area Number 18500242
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neuroscience in general
Research InstitutionKobe University

Principal Investigator

TANIGUCHI Taizo  Kobe University, バイオシグナル研究センター, Visiting Medical Scientist (70346253)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsDementia / Animal model of dementia / Behavioral analysis / Brain and neural function / Medicine / Bioinformatics / Proteome analysis / 疾患モデル
Research Abstract

Proteome analysis of hippocampal proteins from dementia model mice SJLB
Proteins from the hippocampus of dementia model. mice SJLB and control mice are differentiated by 2D gel. Expression level or phosphorylation level of proteins were compared. Several proteins more or less expressed or phosphorylated in SJLB were found and identified by TOF-MS. The results were reported at the Neuro2007 and The 80^th Annual Meeting of The Japanese Pharmacological Science.
Proteome analysis of hippocampal proteins from SJLB before and after donepezil administration.
Proteins from the hippocampus of SJLB before and after donepezil administration were also analyzed. Several proteins changing the level of expression or phosphorylation were identified by TOF-MS. The results were reported at The 81^st Annual Meeting of The Japanese Pharmacological Science.
Analysis of the filament formation mechanism for the tau protein.
The heparin-induced self-aggregation of four repeat peptides (R1-R4) in an acidic solution … More (pH = 4.5) were investigated by fluorescence and circular dichroism (CD) measurements and compared with those in a neutral solution (pH = 7.5). The R1 peptide showed resistive behaviour for filament formation in the acidic solution. This is the first report on the markedly different self-aggregation behaviours of the repeat peptides and published in Biochem. Biophys. Res. Commun. 327, 712-718 (2006); J Biochem. 142, 49-54 (2007)
To examine the linkage-dependent contribution of each repeat peptide (R1-R4) to filament formation of the three- or four-repeat microtubule-binding domain (MBD) in the tau protein, four two-repeat peptides (R12, R13, R23 and R34) and two three-repeat peptides (R123 and R234) were prepared, and their in vitro self-aggregation was investigated. Systematic studies of the linkage-dependent contribution of each repeat peptide to the paired helical filament formation were carried out and the essential information for understanding the mechanism of the filament formation was reported in FEBS J. 275, 1529-39 (2008) Less

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (20 results)

All 2008 2007 2006

All Journal Article (10 results) (of which Peer Reviewed: 4 results) Presentation (10 results)

  • [Journal Article] Linkage-dependent contribution of repeat peptides to self-aggregation of three-or four-repeat microtubule-binding domains in tau protein2008

    • Author(s)
      Kayoko Okuyama
    • Journal Title

      FEBS Journal 275

      Pages: 1529-1539

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Linkage-dependent contribution of repeat peptides to self-aggregation of three- or four-repeat microtubule-binding domains in tau protein2008

    • Author(s)
      Okuyama, K, Nishiura, C, Mizushima, F, Minoura, K, Sumida, M, Taniguchi, T, Tomoo, K, Ishida, T
    • Journal Title

      FEBS J 275(7)

      Pages: 1529-39

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Linkage-dependent contribution of repeat peptides to self-aggregation of three-or four-repeat microtubule-binding domains in tau protein2008

    • Author(s)
      Kayoko Okuyama
    • Journal Title

      FEBS Journal (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Marked Difference Between Self-aggregations of First and Fourth Repeat Peptides on Tau Microtubule-binding Domain in Acidic Solution2007

    • Author(s)
      Fumie Mizushima
    • Journal Title

      J. Biochem 142

      Pages: 49-54

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Marked difference between self-aggregations of first and fourth repeat peptides on tau microtubule-binding domain in acidic solution2007

    • Author(s)
      Mizushima, F, Minoura, K, Tomoo, K, Sumida, M, Taniguchi, T, Ishida, T
    • Journal Title

      J Biochem 142(1)

      Pages: 49-54

    • NAID

      10020097851

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Fluorescence-coupled ('I)conformational monitoring of filament formation of tau microtubule-binding repeat domain2006

    • Author(s)
      Fumie Mizushima
    • Journal Title

      Biochem. Biophys. Res. Commun. 343

      Pages: 712-718

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] 認知症(痴呆)モデルマウスSJLB開発のエピソード2006

    • Author(s)
      谷口 泰造
    • Journal Title

      LABIO21 26

      Pages: 25-29

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Fluorescence-coupled CD conformational monitoring of filament formation of tau microtubule-binding repeat domain2006

    • Author(s)
      Mizushima, F, Minoura, K, Tomoo, K, Sumida, M, Taniguchi, T, Ishida, T
    • Journal Title

      Biochem. Biophys. Res. Commun 327

      Pages: 712-718

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Episodes behind developing the dementia model mice SJLB2006

    • Author(s)
      Taniguchi, T
    • Journal Title

      LABIO 21.26

      Pages: 25-29

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 認知症(痴呆)モデルマウスSJLB開発のエピソード2006

    • Author(s)
      谷口 泰造
    • Journal Title

      LABIO 21 26

      Pages: 25-29

    • Related Report
      2006 Annual Research Report
  • [Presentation] マウス歯状回におけるGABA受容体阻害による長期増強様作用2008

    • Author(s)
      松山 正剛
    • Organizer
      第81回日本薬理学会年会
    • Place of Presentation
      横浜
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ドネペジルを投与したN279K変異トランスジェニックマウス(SJLB)のリン酸化タンパク質プロテオーム解析2008

    • Author(s)
      鷹野 正興
    • Organizer
      第81回日本薬理学会年会
    • Place of Presentation
      横浜
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] LTP-like facilitation via GABA receptors blockade in the mouse dentate gyrus in vivo2008

    • Author(s)
      Matsuyama, S, Kadoyama, K, Matumoto, A, Taniguchi, T
    • Organizer
      The 81st Annual Meeting of The Japanese Pharmacological Science
    • Place of Presentation
      in Yokohama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Phosphoproteome profiling of the Donepezil treated SJLB mice2008

    • Author(s)
      Takano, M, Otani, M, Taniguchi, T
    • Organizer
      The 81st Annual Meeting of The Japanese Pharmacological Science
    • Place of Presentation
      in Yokohama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ドネペジル投与したN279K変異トランスジェニックマウス(SJLB)のリン酸化タンパク質プロテオーム解析2008

    • Author(s)
      鷹野 正興
    • Organizer
      第81回日本薬理学会年会
    • Place of Presentation
      横浜
    • Related Report
      2007 Annual Research Report
  • [Presentation] N279K変異タウトランスジェニックマウス(SJLB)を用いた認知症関連蛋白の探索2007

    • Author(s)
      谷口 泰造
    • Organizer
      第50回神経化学会(Neuro2007)
    • Place of Presentation
      横浜
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] N279K変異タウトランスジェニックマウス(SJLB)のプロテオーム解析2007

    • Author(s)
      鷹野 正興
    • Organizer
      第80回日本薬理学会年会
    • Place of Presentation
      名古屋
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Causative candidates for the dementing diseases analysed by SJLB mice2007

    • Author(s)
      Taniguchi, T, Takano, M, Otani, M, Matsuyama, S
    • Organizer
      Neuro2007
    • Place of Presentation
      in Yokohama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Proteome profiling of the hippocampus in mutant tau(N279K)transgenic mice2007

    • Author(s)
      Takano, M, Otani, M, Sumida, M, Taniguchi, T
    • Organizer
      The 80th, Annual Meeting of The Japanese Pharmacological Science
    • Place of Presentation
      in Nagoya
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] N279K変異タウトランスジェニックマウス(SJLB)を用いた認知症関連蛋白の探索2007

    • Author(s)
      谷口 泰造
    • Organizer
      第50回神経科学会(Neuro2007)
    • Place of Presentation
      横浜
    • Related Report
      2007 Annual Research Report

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Published: 2006-04-01   Modified: 2021-05-31  

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