| Project/Area Number |
18500244
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | Osaka University (2007)
Fukushima Medical University (2006) |
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Principal Investigator |
YASOSHIMA Yasunobu Osaka University, Behavioral Physiology, Associate Professor (00273566)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,590,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | dopamine / medial prefrontal cortex / subthalamic nucleus / c-fos / ドーパミン伝達 / 運動量 / ドーパミン受容体 |
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| Research Abstract |
Inhibition of the medial prefrontal cortex (mPFC) by microinfusion of GABA_A receptor agonist muscimol disrupted both hyperlocomotion and activation of c-fos gene in the subthalamic nucleus (STN) induced by dopaminergic stimulation. These behavioral and neuroanatomical reactions were mediated by dopamine D1-like and D2-like receptors in the mPFC. Many retrogradely labeled pyramidal cells in the mPFC were found following an injection of a retrograde tracer Fluoro-Gold (FG) into the STN. In contrast, anterogradely labeled fibers in the STN were seen after an intra-mPFC injection of biotinylated dextran. Subcutaneous injection of methamphetamine (METH) induced c-fos gene expression in glutamatergic, but not GABAergic, mPFC neurons. Some of c-fos-positive mPFC neurons were also labeled by FG injected into the STN, indicating that some METH-activated mPFC projection neurons monosynaptically innervate the STN. These results suggest that the mPFC projection neurons facilitate dopamine-induced hyperlocomotion via the direct excitatory input to the STN through the corticosubthalamic pathway.
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