Research Project
Grant-in-Aid for Scientific Research (C)
In 2006, we revealed that long-term potentiation (LTP) of parallel fiber(PF) -Purkinje cell synapse in the cerebellum was dependent on increase in calcium level in Purkinje cells. Because the LTP had been previously indicated to be dependent on nitric oxide (NO) signals, the LTP was revealed to be dependent on both Ca^<2+> and NO signals. These results also suggest possible interaction between Ca^<2+> and NO signals.Thus, in 2007, we examined this hypothesis. Application of NO donor evoked increase in Ca^<2+> levels in Purkinje cells in the acute cerebellar slices. Burst stimulation of PF also induced elevation of intracellular Ca^<2+> levels. The PF-induced Ca^<2+> elevation was relatively slow and completely blocked by L-NAME, an antagonist of NO synthetase. These results strongly indicate that NO-dependent increase in Ca^<2+> in Purkinje cells is evoked in physiological condition. Furthermore, involvement of soluble guanylyl cyclase (sGC) was examined. However, bath application of sGC inhibitor, ODQ, had no effect on the NO-dependent Ca^<2+> increase in Purkinje cells.
All 2008 2007 2006 Other
All Journal Article (27 results) (of which Peer Reviewed: 15 results) Presentation (36 results) Book (3 results) Remarks (2 results)
Cerebellum (印刷中)
Cerebellum (in press)
Cerebellum in press(in press)
Journal of Physiology (London) 585
Pages: 549-563
Journal of Neuroscience 27
Pages: 12096-12108
Biochemical and Biophysical Research Communications 363
Pages: 835-839
European Journal of Neuroscience 26
Pages: 2269-2278
Neuropharmacology 52
Pages: 1663-1670
EMBO Journal 26
Pages: 1924-1933
Journal of Physiology 585
In Hormone Handbook(Ed. Urano, A., Nanko-do Press)
Pages: 497-589
Journal of Physiology(London) 585
EMBO Journal 26・7
Neuropharmacology (In press)
Proceedings of the National Academy of Sciences of the United States of America 103
Pages: 8528-8533
Proc. Natl. Aca. Sci. 103・22
http://calcium.cmp.m.u-tokyo.ac.jp/papers.html
