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Modulation of excetability in primary sensory neurons

Research Project

Project/Area Number 18500310
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurophysiology and muscle physiology
Research InstitutionOsaka University

Principal Investigator

KANG Youngnam  Osaka University, Graduate School of Dentistry, Professor (50177755)

Co-Investigator(Kenkyū-buntansha) SAITO Mitsuru  Osaka University, Graduate School of Dentistry, Research Associate (50347770)
SATO Hajime  Osaka University, Graduate School of Dentistry, Research Associate (10432452)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,910,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥3,000,000 (Direct Cost: ¥3,000,000)
KeywordsPersistent Na^+ current / Initiation of action notential / Mesenceohalic triaeminal nucleus / Riluzole / Tetrodotoxin / OX-314 / Axon initial seanent / Spike backpropagation
Research Abstract

It has recently been shown that the persistent Na^+ current (l_NaP) is generated in the proximal axon in response to somatic depolarization in neocortical pyramidal neurons, while the involvement of l_NaP in spike-initiation is still unclear. Here we show a potential role of l_NaP in spike-initiation of primary sensory neurons in the mesencephalic trigeminal nucleus (MTN) that display a backpropagation of the spike initiated in the stem axon toward the soma in response to soma depolarization. Riluzole (10 μM) and tetrodotoxin (10 nM) caused an activation delay or a stepwise increase in the threshold for evoking soma spikes (S-spikes) without affecting the spike itself. Simultaneous patch-clamp recordings from the soma and axon hillock (AH) revealed that bath application of 50 nM tetrodotoxin increased the delay in spike activation in response to soma depolarization, leaving the spike-backpropagation time from the AH to soma unchanged. This indicates that the increase in activation delay occurred in the stem axon. Furthermore, under a decreasing intracellular concentration gradient of QX-314 from the soma to AH created by QX-314-containing and QX-314-free patch pipettes, the amplitude and maximum rate of rise (MRR) of AH-spikes decreased with an increase in the activation delay following repetition of current pulse injections, while S-spikes displayed much less decreases in amplitude and MRR. This suggests that comparing to S-spikes, AH-spikes more accurately reflect the attenuation of axonal-spike by QX-314, consistent with the spike-backpropagation nature. These observations strongly suggest that low-voltage-activated I_NaP is involved in spike-initiation in the stem axon of MTN neurons.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (49 results)

All 2008 2007 2006 Other

All Journal Article (15 results) (of which Peer Reviewed: 6 results) Presentation (31 results) Book (1 results) Remarks (2 results)

  • [Journal Article] Differential Columnar Processing in Local Circuits of Barrel and Insular Cortices2008

    • Author(s)
      Sato, H., et. al.
    • Journal Title

      The Journal of Neuroscience 28(in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] cGMP activates a pH-sensitive leak K^+ current in the presumed cholinergic neuron of basal forebrain.2008

    • Author(s)
      Toyoda, H., et. al.
    • Journal Title

      Journal of Neurophysiology 99(in press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Differential columnar processing in local circuits of barrel and insular cortices2008

    • Author(s)
      Sato, H., Shimanuki, Y., Saito, M., Toyoda, H., Nokubi, T., Maeda, Y., Yamamoto, T., Kang, Y
    • Journal Title

      The Journal of Neuroscience 28

      Pages: 3076-3089

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] cGMP activates a pH-sensitive leak K' current in the presumed cholinergic neuron of basal forebrain2008

    • Author(s)
      Toyoda, H., Saito, M., Sato, H., Dempo, Y., Ohashi, A., Hirai, T., Maeda, Y., Kaneko, T., Kang, Y
    • Journal Title

      ournal of Neuroplrysiology 99

      Pages: 2126-2133

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Nitric oxide activates leak K^+ currents in the presumed cholinergic neuron of basal forebrain.2007

    • Author(s)
      Kang, Y., et. al.
    • Journal Title

      Journal of Neurophysiology 98

      Pages: 3397-3410

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] The involvement of persistent Na^+ current in spike-initiation in primary sensory neurons of the rat mesencephalic trigeminal nucleus2007

    • Author(s)
      Kang, Y., et. al.
    • Journal Title

      Journal of Neurophysiology 97

      Pages: 2385-2393

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Nitric oxide activates leak K^+ currents in the presumed cholinergic neuron of basal forebrain2007

    • Author(s)
      Kang, Y., Dempo, Y., Ohashi, A., Saito, M., Toyoda, H., Sato, H., Koshino, H., Maeda, Y., Hirai, T
    • Journal Title

      Journal of Neurophysiology 98

      Pages: 3397-3410

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Involvement of persistent Na' current in spike initiation in primary sensory neurons of the rat mesencephalic trigeminal nucleus2007

    • Author(s)
      Kang, Y., Saito, M., Sato, H., Toyoda, H., Maeda, Y., Hirai, T., Bae, Y.C
    • Journal Title

      Journal of Neurophysiology 97

      Pages: 2385-2393

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] The involvement of persistent Na^+ current in spike-initiation in primary sensory neurons of the rat mesencephalic trigeminal nucleus2007

    • Author(s)
      Kang, Y. et al.
    • Journal Title

      Journal of Neurophysiology (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Two opposing roles of 4-AP-sensitive K^+ current in initiation and invasion of spikes in rat mesencephalic trigeminal neurons2006

    • Author(s)
      Saito, M., et. al.
    • Journal Title

      Journal of Neurophysiology 96(4)

      Pages: 1887-1901

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Functional roles of 5-HT_<3/4> receptors in neurons of rat dorsal motor nucleus of the vagus2006

    • Author(s)
      Fukushi, E., et. al.
    • Journal Title

      Neuroscience 141(2)

      Pages: 675-685

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Two opposing roles of 4-AP-sensitive K current in initiation and invasion of spikes in rat mesencephalic trigeminal neurons2006

    • Author(s)
      Saito, M., Murai, Y., Sato, H., Bae, Y.C., Akaike, T., Takada, M., Kang, I
    • Journal Title

      Journal of Neurophysiology 96

      Pages: 1887-1901

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Functional roles of 5-HT_3/4 receptors in neurons of rat dorsal motor nucleus of the vagus2006

    • Author(s)
      Fukushi, E., Saito, M., Sato, H., Endo, T, Hamaue, N., Hirafuji, M., Minami, M., Kang, Y
    • Journal Title

      Neuroscience 141

      Pages: 675-685

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Two opposing roles of 4-AP-sensitive K^+ current in initiation and invasion of spikes in rat mesencephalic trigeminal neurons2006

    • Author(s)
      Saito, M. et al.
    • Journal Title

      Journal of Neurophysiology 96(4)

      Pages: 1887-1901

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Functional roles of 5-HT_<3/4> receptors in neurons of rat dorsal motor nucleus of the vagus2006

    • Author(s)
      Fukushi, E. et al.
    • Journal Title

      Neuroscience 141(2)

      Pages: 675-685

    • Related Report
      2006 Annual Research Report
  • [Presentation] Differential involvement of GABAergic inhibition in intra-columnar and inter-columnar communication between barrel and insular cortices2008

    • Author(s)
      Kang, Y.
    • Organizer
      2nd International Conference of Neurons and Brain Disease
    • Place of Presentation
      University of Toronto, Toronto, Canada
    • Year and Date
      2008-08-30
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Phospholipase C-related catalytically inactive protein(PRIP)regulates GABA_A responses in the barrel cortex2007

    • Author(s)
      Hirao, K., et. al.
    • Organizer
      Society for Neuroscience 37th Annual Meeting
    • Place of Presentation
      San Diego Convention Center, San Diego, CA, USA
    • Year and Date
      2007-11-05
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Phospholipase C-related catalytically inactive protein (PRIP) regulates GABAA responses in the barrel cortex2007

    • Author(s)
      Hirao, K., Ogawa, T., Toyoda, IL, Saito, M., Sato, H., Kanematsu, T., Hirata, M., Kang, Y
    • Organizer
      Society for Neuroscience 37th Annual Meeting
    • Place of Presentation
      an Diego Convention Center, San Diego, CA, USA
    • Year and Date
      2007-11-05
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Phospholipase C-related catalytically inactive protein (PRIP) regulates GABA_A, responses in the barrel cortex2007

    • Author(s)
      Hirao, K., et. al.
    • Organizer
      Society for Neuroscience 37th Annual Meeting
    • Place of Presentation
      San Diego Convention Center, San Diego, CA, USA
    • Year and Date
      2007-11-05
    • Related Report
      2007 Annual Research Report
  • [Presentation] Nitric oxide activates TASK1-like leak K^+ channels in the presumed cholinergic neuron of basal forebrain2007

    • Author(s)
      Toyoda, H., et. al.
    • Organizer
      Society for Neuroscience 37th Annual Meeting
    • Place of Presentation
      San Diego Convention Center, San Diego, CA, USA
    • Year and Date
      2007-11-04
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Nitric oxide activates leak K^+ currents in the presumed cholinergic neuron of basal forebrain2007

    • Author(s)
      Sato, H., et. al.
    • Organizer
      Society for Neuroscience 37th Annual Meeting
    • Place of Presentation
      San Diego Convention Center, San Diego, CA, USA
    • Year and Date
      2007-11-04
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Nitric oxide activates TASKI-like leak K channels in the presumed cholinergic neuron of basal forebrain2007

    • Author(s)
      Toyoda, H., Saito, M., Sato, H., Dempo, Y., Ohashi, A, Hirai, T., Maeda, Y, Kaneko, T., Kang, Y
    • Organizer
      Society for Neuroscience 37th Annual Meeting
    • Place of Presentation
      San Diego Convention Center, San Diego, CA, USA
    • Year and Date
      2007-11-04
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Interaction between h-channels and glutamate receptor channels in the mesencephalic trigeminal neurons2007

    • Author(s)
      Sato, H., et. al.
    • Organizer
      第30回 日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2007-09-12
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Roles of phospholipase C-related inactive protein(PRIP)in modulating GABA_A IPSC_S2007

    • Author(s)
      Toyoda, H., et. al.
    • Organizer
      第30回 日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2007-09-11
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Roles of phospholipase C-related inactive protein (PRIP) in modulating GABA_AIPSC_s2007

    • Author(s)
      Toyoda, H., et. al.
    • Organizer
      第30回 日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2007-09-11
    • Related Report
      2007 Annual Research Report
  • [Presentation] Differential involvement of GABA_A IPSP_s in inter-columnar communication in the barrel and insular cortices2007

    • Author(s)
      Saito, M., et. al.
    • Organizer
      第30回 日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2007-09-10
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Differential involvement of GABA_AIPSP_s in inter-columnar communication in the barrel and insular cortices2007

    • Author(s)
      Saito, M., et. al.
    • Organizer
      第30回 日本神経科学大会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2007-09-10
    • Related Report
      2007 Annual Research Report
  • [Presentation] ラット三叉神経中脳路核ニューロンに発現するGABA_A受容体分子の輸送におけるPKCの関与2007

    • Author(s)
      齋藤 充, 他
    • Organizer
      第48回 歯科基礎医学会
    • Place of Presentation
      北海道大学(北海道札幌市)
    • Year and Date
      2007-08-31
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] 咬合力の超精細調節を可能にする神経回路の秘密2007

    • Author(s)
      姜 英男
    • Organizer
      第18回 日本咀嚼学会学術大会
    • Place of Presentation
      千里ライフサイエンスセンター(大阪府吹田市)
    • Year and Date
      2007-08-25
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Interaction between hyperlarization-activated channels and glutamtereceptor channels in neurons of the mesencephalic trigeminal nucleus2007

    • Author(s)
      Saito, M., et. al.
    • Organizer
      第84回 日本生理学会大会
    • Place of Presentation
      大阪国際交流センター(大阪府大阪市)
    • Year and Date
      2007-03-22
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Modulation of GABA IPSCs by phospholipase C-related inactive protein-1/2(PRIP-1/2)in the barrel cortex2007

    • Author(s)
      Toyoda, H., et. al.
    • Organizer
      第84回 日本生理学会大会
    • Place of Presentation
      大阪国際交流センター(大阪府大阪市)
    • Year and Date
      2007-03-21
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Differential involvement of GABA_A IPSPs in inter-columnar communication in the barrel and insular cortices2007

    • Author(s)
      Sato, H., et. al.
    • Organizer
      第84回 日本生理学会大会
    • Place of Presentation
      大阪国際交流センター(大阪府大阪市)
    • Year and Date
      2007-03-21
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] A key role of the mesencephalic trigeminal neuron in modulating mastication2006

    • Author(s)
      Kang, Y.
    • Organizer
      The 6th Congress of the Federation of Asian and Oceanian Physiological Societies
    • Place of Presentation
      JW Marriott Hotel, Seoul, Korea
    • Year and Date
      2006-10-17
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Differential involvement of GABA_A IPSPs in intercolumnar communication in the barrel and insular cortices2006

    • Author(s)
      Kang, Y., et. al.
    • Organizer
      Society for Neuroscience 36th Annual Meeting
    • Place of Presentation
      Georgia World Congress Center, Atlanta, GA, USA
    • Year and Date
      2006-10-16
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Differential involvement of GABAA IPSPs in intercolumnar communication in the barrel and insular cortices2006

    • Author(s)
      Kang, Y., Shimanuki, Y., Saito, M., Sato, H., Toyoda, H
    • Organizer
      Society for Neuroscience 36th Annual Meeting
    • Place of Presentation
      Georgia World Congress Center, Atlanta, GA, USA
    • Year and Date
      2006-10-16
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 三叉神経中脳路核ニューロンにおけるhチャネルとグルタミン酸受容体チャネルの相互作用2006

    • Author(s)
      齋藤 充, 他
    • Organizer
      第48回 歯科基礎医学会大会
    • Place of Presentation
      鶴見大学(神奈川県横浜市)
    • Year and Date
      2006-09-22
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ラット軟口蓋体性感覚の大脳皮質での情報処理機構2006

    • Author(s)
      奥田 義彦, 他
    • Organizer
      第48回 歯科基礎医学会大会
    • Place of Presentation
      鶴見大学(神奈川県横浜市)
    • Year and Date
      2006-09-22
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 三叉神経運動核咬筋領域内のα及びγ運動ニューロンの電気生理学的分類2006

    • Author(s)
      太田 雅裕, 他
    • Organizer
      第48回 歯科基礎医学会大会
    • Place of Presentation
      鶴見大学(神奈川県横浜市)
    • Year and Date
      2006-09-21
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 顎口腔感覚入力による上肢外転筋運動単位動員阻害2006

    • Author(s)
      佐藤 元, 他
    • Organizer
      第48回 歯科基礎医学会大会
    • Place of Presentation
      鶴見大学(神奈川県横浜市)
    • Year and Date
      2006-09-21
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ラット三叉神経運動核咬筋領域内に存在するα及びγ運動ニューロンの電気生理学的分類2006

    • Author(s)
      太田 雅裕, 他
    • Organizer
      第99回 近畿生理学談話会
    • Place of Presentation
      大阪歯科大学(大阪府枚方市)
    • Year and Date
      2006-09-21
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 嚥下に関与するラット軟口蓋体性感覚の大脳皮質での情報処理様式2006

    • Author(s)
      奥田 義彦, 他
    • Organizer
      第99回 近畿生理学談話会
    • Place of Presentation
      大阪歯科大学(大阪府枚方市)
    • Year and Date
      2006-09-21
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] ラット大脳皮質味覚野における情報処理の特異性2006

    • Author(s)
      島貫 靖士, 他
    • Organizer
      第115回 日本補綴歯科学会学術大会
    • Place of Presentation
      札幌コンベンションセンターSORA(北海道札幌市)
    • Year and Date
      2006-07-09
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] A possible key role of mesencephalic trigeminal neurons in α-γ linkage during mastication2006

    • Author(s)
      Kang, Y.
    • Organizer
      International Mastication Symposium-A Satellite Symposium of the IADR 84th General Session
    • Place of Presentation
      Brisbane, Australia
    • Year and Date
      2006-06-26
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] A possible key role of mesencephalic trigeminal neurons in a-y linkage during mastication2006

    • Author(s)
      Kang, Y
    • Organizer
      International Mastication Symposium -A Satellite Symposium of the IADR 84th General Session
    • Place of Presentation
      Brisbane, Australia
    • Year and Date
      2006-06-26
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Voluntary teeth clenching suppresses motor unit recruitment during increasing isometric muscle contractions on the arm abduction2006

    • Author(s)
      Sato, H., et. al.
    • Organizer
      84th General Session & Exhibition of the IADR
    • Place of Presentation
      Brisbane, Australia
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Voluntary teeth clenching suppresses motor unit recruitment during increasing isometric muscle contractions on the ann abduction2006

    • Author(s)
      Sato, H., Saito, M., Kang, Y
    • Organizer
      84th General Session & Exhibition of the IADR
    • Place of Presentation
      Brisbane, Australia
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 基礎歯科生理学 第5版2008

    • Author(s)
      森本 俊文, 他 編
    • Total Pages
      466
    • Publisher
      医歯薬出版株式会社
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Remarks] 「研究成果報告書概要(和文)」より

    • URL

      http://www.dent.osaka-u.ac.jp/~phys/

    • Related Report
      2007 Final Research Report Summary
  • [Remarks]

    • URL

      http://www.dent.osaka-u.ac.jp/~phys/

    • Related Report
      2007 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

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