Analysis for functional neuronal network of developing cerebellum using cerebellum-pons-medulla preparation.

• Project/Area Number: 18500318
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Neurophysiology and muscle physiology
• Research Institution: Hyogo College of Medicine (2008); The Institute of Physical and Chemical Research (2006-2007)
• Principal Investigator: ARATA Akiko Hyogo College of Medicine, 医学部, 准教授 (00266082)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,020,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
• Keywords: 摘出脳ブロック標本 / 光学的測定法 / 蛍光トレーサー / 小脳神経回路形成 / 摘出小脳-橋-延髄標本 / 機能的神経回路 / 呼吸活動 / 電気生理学的手法 / 下オリーブ核 / 橋傍腕結合核 / 活動依存的シナプス形成 / 橋傍結合腕核 / LTD / 免疫組織化学法 / 呼吸

Research Abstract

下オリーブ核から小脳への投射様式や平行線維の発達過程を蛍光トレーサーや免疫組織化学法を用いて調べた結果、P5-P7 の生後1 週の小脳においてLTD が発現する場所には苔状線維から入力を受けた平行線維と下オリーブ核の両方から入力を受けているプルキンエ細胞が存在し、機能的神経回路を作り始めていることが分かった。また、呼吸性活動をトリガーとして光学的測定法を行なった結果より、同じ呼吸相の活動が登上線維からと平行線維から小脳へ向かっていることが示唆された。これは、呼吸性活動が小脳神経回路の発達・形成に関与する可能性を示唆する。呼吸性活動は、発達初期に、小脳神経回路の活動依存的シナプス形成に深く関わっていると考えられた。

Research Products

• [Journal Article] DSCAM deficiency causes loss of pre-inspiratory neuron synchroneity and perinatal death. (2009)
  • Author(s): Amano K, Fujii M, Arata S, Tojima T, Ogawa M, Morita N, Shimohata A, Furuichi T, Itohara S, Kamiguchi H, Korenberg JR, Arata A, Yamakawa K.
  • Journal Title: J. Neurosci. 29 Pages: 2984-2996
  • Peer Reviewed
• [Journal Article] DSCAM deficiency causes loss of pre-inspiratory neuron synchroneity and perinatal death. (2009)
  • Author(s): Amano, K., Fujii, M., Arata, S., Ogawa, M., Arata, A., Yamakawa, K., 他7名
  • Journal Title: Journal of Neuroscience 29 Pages: 2984-2996
  • Peer Reviewed
• [Journal Article] Catecholaminergic modulation of the respiratory rhythm generator in the isolated brainstem-spinal cord preparation from neonatal rat. (2008)
  • Author(s): Arata A, Fujii M.
  • Journal Title: Adv Exp Med Biol. 605 Pages: 83-87
  • Peer Reviewed
• [Journal Article] Catecholaminergic modulation of the respiratory rhythm generator in the isolated brainstem-spinal cord preparation from neonatal rat. (2008)
  • Author(s): Arata, A., Fujii, M.
  • Journal Title: Advances in experimental medicine & biology. 605 Pages: 83-87
  • Peer Reviewed
• [Journal Article] Respiratory activity in brainstem of fetal mice lacking glutamate decarboxylase 65/67 and vesicular GABA transporter. (2007)
  • Author(s): Fujii M, Arata A, Kanbara-Kume N, Saito K, Yanagawa Y, Obata K.
  • Journal Title: Neuroscience. 146 Pages: 1044-1052
  • Peer Reviewed
• [Journal Article] Respiratory activity in brainstem of fetal mice lacking glutamate decarboxylase 65/67 and vesicular γ-aminobutyric acid transporter. (2007)
  • Author(s): Fujii, M., Arata, A., Kanbara-Kume, N., Saito, K., Yanagawa, Y., Obata, K.
  • Journal Title: Neuroscience 146 Pages: 1044-1052
  • Peer Reviewed
• [Journal Article] Adrenaline contributes to prenatal respiratory maturation in rat medulla-spinal cord preparation. (2006)
  • Author(s): Fujii M, Umezawa K, Arata A.
  • Journal Title: Brain Res. 1090 Pages: 45-50
  • Peer Reviewed
• [Journal Article] Dopamine desynchronizes the pace-making neuronal activity of rat respiratory rhythm generation. (2006)
  • Author(s): Fujii M, Umezawa K, Arata A.
  • Journal Title: Eur J Neurosci 23 Pages: 1015-1027
  • Peer Reviewed
• [Journal Article] Adrenaline contributes to prenatal respiratory maturation in rat medulla-spinal cord preparation. (2006)
  • Author(s): Fujii, M, Umezawa, K, Arata, A
  • Journal Title: Brain Research 1090 Pages: 45-50
• [Journal Article] Dopamine desynchronizes the pace-making neuronal actibity ob rat respiratory thythm generation (2006)
  • Author(s): Fujii, M, Umezawa, K, Arata, A
  • Journal Title: European Journal of Neuroscience 23 Pages: 1015-1027
• [Presentation] Adrenaline contributes to respiratory neural network maturation in developing rat medulla-spinal cord preparation (2008)
  • Author(s): Arata, A., Fujii, M., Matsunaga, W., Obata, K.
  • Organizer: 6^<th> Forum of European Neuroscience
  • Place of Presentation: Geneva, Switzerland
• [Presentation] Respiratory activities presumably play roles in the activity-dependent formation of neuronal networks in the developing cerebellum. (2008)
  • Author(s): Arata, A., Fujii, M., Ito, M.
  • Organizer: The 31st Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Tokyo
• [Presentation] Abnormal facial nucleus activity in Dscam1-deficient mouse : A model for sudden infant death syndrome. (2008)
  • Author(s): Amano, K., Fujii, M., Arata, S., Ogawa, M., Itohara, S., Arata, A., Yamakawa, K.
  • Organizer: The 31^<st> Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Tokyo
• [Presentation] Optical imaging analysis on the memory trace in zebrafish. (2008)
  • Author(s): Aoki, T., Agetsuma, M., Aizawa, H., Arata, A., Higashijima, S., Okamoto, H.
  • Organizer: The 31^<st> Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Tokyo
• [Presentation] Central hypoventilation caused by abnormalities of respiratory network in Dscam1 deficient mouse. (2008)
  • Author(s): Arata, A., Fujii, M., Amano, K., Arata, S., Yamakawa, K.
  • Organizer: The 85th Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Tokyo
• [Presentation] Respiratory activities presumably play roles in the activity-dependent formation of neuronal networks in the developing cerebellum. (2008)
  • Author(s): Arata, A., Fujii, M., Ito, M.
  • Organizer: The 31^<st> Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 東京
• [Presentation] Abnormal facial nucleus activity in Dscaml-deficient mouse : A model for sudden infant death syndrome. (2008)
  • Author(s): Amano, K., Fujii, M., Arata, S., Ogawa, M., Itohara, S., Arata, A., Yamakawa, K.
  • Organizer: The 31^<st> Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 東京
• [Presentation] Optical imaging analysis on the memory trace in zebrafish. (2008)
  • Author(s): Aoki, T., Agetsuma, M., Aizawa, H., Arata, A., Higashijima, S., Okamoto, H.
  • Organizer: The 31^<st> Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: 東京
• [Presentation] Adrenaline contributes to respiratory neural network maturation in developing rat medulla-spinal cord preparation. (2008)
  • Author(s): Arata, A., Fujii, M., Matsunaga, W., Obata, K.
  • Organizer: 6^<th> Forum of European Neuroscience
  • Place of Presentation: Geneva, Switzerland
• [Presentation] Neonatal cerebellum broadly displays respiratory activities (2007)
  • Author(s): Arata, A., Fujii, M., Ito, M.
  • Organizer: The 30st Annual Meeting of the Japan (Neuroscience Society (Neuro2007)
  • Place of Presentation: Yokohama
• [Presentation] Respiratory activities optically recorded from the newborn rat cerebellum and related medullary structures (2007)
  • Author(s): Arata, A., Fujii, M., Ito, M.
  • Organizer: The 84^<th> Annual Meeting of the Physiological Society of Japan
  • Place of Presentation: Osaka
• [Presentation] Respiratory activities optically recorded from the newborn rat cerebellum and related medullary structures. (2007)
  • Author(s): Arata, A., Fujii, M., Ito, M.
  • Organizer: 84^<th> Annual Meeting of Physiological Science Japan
  • Place of Presentation: 大阪
• [Presentation] Parallel fiber-evoked long-lasting burst in Purkinje cells of the neonatal cerebelum and its dependence on P/Q type Ca^<2+>channels. (2007)
  • Author(s): Arata, A., Fujii, M., Matsunaga, W., Yabunaka, A., Ito, M.
  • Organizer: IBRO World Congress of Neuroscience2007
  • Place of Presentation: Melbourne
• [Presentation] Neonatal cerebellum brodaly displays respiratory activities. (2007)
  • Author(s): Arata, A., Fujii, M., Ito, M.
  • Organizer: 30^<th> Annual Meeting of the Japan Neuroscience Society(Neuro2007)
  • Place of Presentation: 横浜
• [Presentation] Loss of pre-inspiratory neuron synchroneity in mice with Dscaml deficiency. (2007)
  • Author(s): Amano, K., fujii, M., Tojima, T., Ogawa, M., Morita, N., Arata, S., Shimohata, A., Furuichi, T., Itohara, S., Kamiguchi, H., Korenberg, J. R., Arata, A., Yamakawa, K.
  • Organizer: 37^<th> Annual Meeting of Society for Neuroscience
  • Place of Presentation: San Diego
• [Presentation] The prolonged responses of Purkinje cells in the flocculus/paraflocculus regions of the developing cerebellum. (2006)
  • Author(s): Arata, A., Fujii, M., Matsunaga, W., Ito, M.
  • Organizer: 36^<th> Annual Meeting Soc. Neurosci.
  • Place of Presentation: Atlanta
• [Presentation] Orexin-containing beaded cerebellar afferents mediate effects of stimulation of hypothalamic defense area on flocculus Purkinje cells in rabbits. (2006)
  • Author(s): Nisimaru, N., Mittal, C., Shirai, Y., Sooksawate, T. Gupta, M., Arata, A., Sakurai, T., Yamamoto, M., Ito, M.
  • Organizer: 36^<th> Annual Meeting Soc. Neurosci.
  • Place of Presentation: Atlanta
• [Presentation] Modulation of catecholamine on respiratory rhythm generator in isolated brainstem-spinal cord preparation from neonatal rat. (2006)
  • Author(s): Arata, A., Fujii, M.
  • Organizer: Oxford Conference 2006, Lake Louise
  • Place of Presentation: Alberta Canada
• [Presentation] Maintained GABA content and reduced neuronal activity in the fetal mouse brain lacking vesicular GABA transporter. (2006)
  • Author(s): Obata, K., Arata, A., Saito, K., Hirono, M., Yanagawa, Y.
  • Organizer: 5^<th> Forum of European Neuroscience
  • Place of Presentation: Vienna, Austria
• [Presentation] Parallel development of P/Q type Ca channels, primary dendrites and prolonged response of Purkinje cell in developing cerebellum. (2006)
  • Author(s): Matsunaga, W., Arata, A., Fujii, M., Ito, M.
  • Organizer: 29^<th> Annual Meeting Japan Neurosci. Soc.
  • Place of Presentation: Kyoto
• [Presentation] Decrease in spontaneous activity and cell death of the brachial motoneurons in GAD67-deficient mouse embryo. (2006)
  • Author(s): Arata, A., Obata, K., Davies, J., Bellingham, M., Noakes, P.G.
  • Organizer: 29^<th> Annual Meeting of the Japan Neuroscience Society
  • Place of Presentation: Kyoto
• [Presentation] Abnormality of central respiratory network in Dscam knock-out mouse. (2006)
  • Author(s): Fujii, M., Amano, K., Arata, S., Shimohata, A., Yamakawa, K., Arata, A.
  • Organizer: 29th Annual Meeting of Japan Neurosci, Soc.
  • Place of Presentation: Kyoto
• [Book] Integration in Respiratory Control-from Genes to Systems (2008)
  • Author(s): Arata, A. and Fujii, M.
  • Total Pages: 541
  • Publisher: Advances in Experimental Medicine and Biology Series, Springer Publishers
• [Patent(Industrial Property Rights)] 特 許 (2006)
  • Inventor(s): 山川 和弘, 天野 賢治, 藤井 盛光, 荒田 晶子
  • Industrial Property Rights Holder: 理化学研究所
  • Industrial Property Number: 2006-066489
  • Filing Date: 2006-03-10