Analysis for functional neuronal network of developing cerebellum using cerebellum-pons-medulla preparation.
Project/Area Number |
18500318
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Neurophysiology and muscle physiology
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Research Institution | Hyogo College of Medicine (2008) The Institute of Physical and Chemical Research (2006-2007) |
Principal Investigator |
ARATA Akiko Hyogo College of Medicine, 医学部, 准教授 (00266082)
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Project Period (FY) |
2006 – 2008
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Project Status |
Completed (Fiscal Year 2008)
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Budget Amount *help |
¥4,020,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥420,000)
Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
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Keywords | 摘出脳ブロック標本 / 光学的測定法 / 蛍光トレーサー / 小脳神経回路形成 / 摘出小脳-橋-延髄標本 / 機能的神経回路 / 呼吸活動 / 電気生理学的手法 / 下オリーブ核 / 橋傍腕結合核 / 活動依存的シナプス形成 / 橋傍結合腕核 / LTD / 免疫組織化学法 / 呼吸 |
Research Abstract |
下オリーブ核から小脳への投射様式や平行線維の発達過程を蛍光トレーサーや免疫組織化学法を用いて調べた結果、P5-P7 の生後1 週の小脳においてLTD が発現する場所には苔状線維から入力を受けた平行線維と下オリーブ核の両方から入力を受けているプルキンエ細胞が存在し、機能的神経回路を作り始めていることが分かった。また、呼吸性活動をトリガーとして光学的測定法を行なった結果より、同じ呼吸相の活動が登上線維からと平行線維から小脳へ向かっていることが示唆された。これは、呼吸性活動が小脳神経回路の発達・形成に関与する可能性を示唆する。呼吸性活動は、発達初期に、小脳神経回路の活動依存的シナプス形成に深く関わっていると考えられた。
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Report
(4 results)
Research Products
(34 results)
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[Journal Article] DSCAM deficiency causes loss of pre-inspiratory neuron synchroneity and perinatal death.2009
Author(s)
Amano K, Fujii M, Arata S, Tojima T, Ogawa M, Morita N, Shimohata A, Furuichi T, Itohara S, Kamiguchi H, Korenberg JR, Arata A, Yamakawa K.
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Journal Title
J. Neurosci. 29
Pages: 2984-2996
Related Report
Peer Reviewed
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[Journal Article] DSCAM deficiency causes loss of pre-inspiratory neuron synchroneity and perinatal death.2009
Author(s)
Amano, K., Fujii, M., Arata, S., Ogawa, M., Arata, A., Yamakawa, K., 他7名
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Journal Title
Journal of Neuroscience 29
Pages: 2984-2996
Related Report
Peer Reviewed
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[Journal Article] Respiratory activity in brainstem of fetal mice lacking glutamate decarboxylase 65/67 and vesicular γ-aminobutyric acid transporter.2007
Author(s)
Fujii, M., Arata, A., Kanbara-Kume, N., Saito, K., Yanagawa, Y., Obata, K.
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Journal Title
Neuroscience 146
Pages: 1044-1052
Related Report
Peer Reviewed
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[Presentation] Abnormal facial nucleus activity in Dscam1-deficient mouse : A model for sudden infant death syndrome.2008
Author(s)
Amano, K., Fujii, M., Arata, S., Ogawa, M., Itohara, S., Arata, A., Yamakawa, K.
Organizer
The 31^<st> Annual Meeting of the Japan Neuroscience Society
Place of Presentation
Tokyo
Related Report
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[Presentation] Optical imaging analysis on the memory trace in zebrafish.2008
Author(s)
Aoki, T., Agetsuma, M., Aizawa, H., Arata, A., Higashijima, S., Okamoto, H.
Organizer
The 31^<st> Annual Meeting of the Japan Neuroscience Society
Place of Presentation
Tokyo
Related Report
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[Presentation] Abnormal facial nucleus activity in Dscaml-deficient mouse : A model for sudden infant death syndrome.2008
Author(s)
Amano, K., Fujii, M., Arata, S., Ogawa, M., Itohara, S., Arata, A., Yamakawa, K.
Organizer
The 31^<st> Annual Meeting of the Japan Neuroscience Society
Place of Presentation
東京
Related Report
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[Presentation] Optical imaging analysis on the memory trace in zebrafish.2008
Author(s)
Aoki, T., Agetsuma, M., Aizawa, H., Arata, A., Higashijima, S., Okamoto, H.
Organizer
The 31^<st> Annual Meeting of the Japan Neuroscience Society
Place of Presentation
東京
Related Report
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[Presentation] Loss of pre-inspiratory neuron synchroneity in mice with Dscaml deficiency.2007
Author(s)
Amano, K., fujii, M., Tojima, T., Ogawa, M., Morita, N., Arata, S., Shimohata, A., Furuichi, T., Itohara, S., Kamiguchi, H., Korenberg, J. R., Arata, A., Yamakawa, K.
Organizer
37^<th> Annual Meeting of Society for Neuroscience
Place of Presentation
San Diego
Related Report
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[Presentation] Orexin-containing beaded cerebellar afferents mediate effects of stimulation of hypothalamic defense area on flocculus Purkinje cells in rabbits.2006
Author(s)
Nisimaru, N., Mittal, C., Shirai, Y., Sooksawate, T. Gupta, M., Arata, A., Sakurai, T., Yamamoto, M., Ito, M.
Organizer
36^<th> Annual Meeting Soc. Neurosci.
Place of Presentation
Atlanta
Related Report
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[Presentation] Maintained GABA content and reduced neuronal activity in the fetal mouse brain lacking vesicular GABA transporter.2006
Author(s)
Obata, K., Arata, A., Saito, K., Hirono, M., Yanagawa, Y.
Organizer
5^<th> Forum of European Neuroscience
Place of Presentation
Vienna, Austria
Related Report
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[Presentation] Abnormality of central respiratory network in Dscam knock-out mouse.2006
Author(s)
Fujii, M., Amano, K., Arata, S., Shimohata, A., Yamakawa, K., Arata, A.
Organizer
29th Annual Meeting of Japan Neurosci, Soc.
Place of Presentation
Kyoto
Related Report
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