| Project/Area Number |
18500323
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | Hokkaido University |
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Principal Investigator |
SASAKI Nobuya Hokkaido University, Grad. School of Vet. Med., Associate Professor (20302614)
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| Co-Investigator(Kenkyū-buntansha) |
AGUI Takashi Hokkaido University, Grad. School of Vet. Med., Professor (00212457)
MIYOSHI Ichiro Nagoya City University, Grad. School of Med. Sci., Associate Professor (10183972)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥4,230,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥630,000)
Fiscal Year 2007: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | QTL mapping / Telomere / マウス |
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| Research Abstract |
Telomere is the nucleoprotein structure at the end of eukaryotic chromosome. Telomere length varies among species, and even in strains in mice. In this study, we screened the telomere length polymorphism among nine wild-derived inbred strains that are recently established mice stocks, and found that KJR/Ms (KJR) mice have extreme short telomeres (about 5 kb). To analyze the genetic loci contributing short telomere phenotype in KJR mice, we crossed KJR mice and C57BL/6J (B6J) mice, standard laboratory strain that possess long telomeres (>40 kb). F_1 mice showed bimodal telomere signals, indicating that longer set of telomeres is inherited from B6J and shorter one is from KJR mice. The shorter set of telomeres was elongated by B6J allele compared with the one of KJR parent, indicating that the phenotype of KJR is recessive. Backcross progenies to KJR mice showed the multiple length of telomeres, indicating that multiple loci of B6J allele contribute to elongate the short telomeres of KJR mice. Quantitative trait loci (QTL) analysis revealed that the genetic loci for contributors to short telomeres in KJR mice were mapped on chromosome 13 and 3. Interestingly, both loci include two core components of telomerase (mTert and mTerc), indicating that the telomerase in KJR mice may affect their short telomere phenotype.
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