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The functional analysis of Erc gene encoding GPI anchor protein

Research Project

Project/Area Number 18500330
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Laboratory animal science
Research InstitutionJuntendo University

Principal Investigator

ZHANG Danqing  Juntendo University, SCHOOL OF MEDICINE, ASSISTANT PROFESSOR (40296877)

Co-Investigator(Kenkyū-buntansha) KOBAYASHI Toshiyuki  JUNTENDO UNIVERSITY, GRADUATE SCHOOL OF MEDICINE, ASSOCIATE PROFESSOR (40260070)
HINO Okio  JUNTENDO UNIVERSITY, SCHOOL OF MEDICINE, PROFESSOR (90127910)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,110,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsErc / Mesothelin gene / Tsc2 gene / GPI anchor protein / knockout mouse / Carcinogenesis / Erc遺伝子
Research Abstract

The Erc (expressed in renal carcinoma) was identified as an inducible gene during the renal carcinogenesis of the Eker (Tsc2 mutant) rat and is a homologue of the human Mesothelin gene. Erc is also overexpressed in the renal carcinoma cell line of the Tsc2 knockout (KO) mouse. To elucidate functional importance of Erc in renal carcinogenesis as well as in mesothelioma, we have performed genetic cross experiments between Tsc2 KO and Erc KO mice. Then we examined influence of the Erc genotype on the development of renal carcinomas in the Tsc2 hetero-mutant mouse. In 32% of male and 20% of female Tsc2 hetero-mutant mice with wild-type Erc, renal carcinomas with a diameter ranging from 4 to 30mm were developed by the age of 18 months. In contrast, all of the renal carcinomas in Tsc2 hetero-mutant mice with Erc homo-mutation had a diameter equal to or less than 2mm. There was no remarkable difference in the size of renal tumors between Tsc2 hetero-/Erc hetero-mutant and Tsc2 hetero-mutant/Erc wild-type mice. These results suggest that Erc may play an important role for tumor proliferation. In addition, we established Tsc2/Erc-deficient renal carcinoma cell lines and then transferred Erc-expression system into them. Adhesion of cell lines to the collagen-coated plate became stronger by overexpression of Erc protein. On the other hand, cell adhesive property was deteriorated when Erc expression was inhibited by RNA interference, suggesting that Erc has a role in the cell-substrate adhesion. Now we are further investigating in vivo function of Erc by implanting the renal carcinoma cell lines into the nude mouse.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (35 results)

All 2008 2007 2006

All Journal Article (25 results) (of which Peer Reviewed: 11 results) Presentation (10 results)

  • [Journal Article] MESOMARK kit detects C-ERC/mesothelin,but not SMRP with C-terminus2008

    • Author(s)
      Segawa T, et. al.
    • Journal Title

      Biochem Biophys Res Commun. 369(3)

      Pages: 915-8

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] siRNA-mediated Erc gene silencing suppresses tumor growth in Tsc2 mutant renal carcinoma model2008

    • Author(s)
      Imamura O, et. al.
    • Journal Title

      Cancer Letters (In press)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Sensitive and Specific New Enzyme-Linked Immunosorbent Assay for N-ERC/Mesothelin Increases its Potential as a Useful Serum Tumor Marker for Mesothelioma2008

    • Author(s)
      Shiomi K, et. al.
    • Journal Title

      Clin Cancer Res. 14(5)

      Pages: 1431-7

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] MESOMARK kit detects C-ERC/mesothelin, but not SMRP with C-terminus2008

    • Author(s)
      Segawa, T, et. al.
    • Journal Title

      Biochem Biophys Res Commun 369(3)

      Pages: 915-8

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] siRNA-mediated Erc gene silencing suppresses tumor growth in Tsc2 mutant renal carcinoma model2008

    • Author(s)
      Imamura, O, et. al.
    • Journal Title

      Cancer Letters(Reviewed) (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Sensitive and Specific New Enzyme-Linked Immunosorbent Assay for N-ERC/Mesothelin Increases its Potential as a Useful Serum Tumor Marker for Mesothelioma2008

    • Author(s)
      Shiomi, K, et. al.
    • Journal Title

      Clin Cancer Res (14) 5

      Pages: 1431-7

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] MESOMARK kit detects C-ERC/mesothelin,but not SMRP with C-terminus2008

    • Author(s)
      Segawa T, et. al.
    • Journal Title

      Biochem Biophys Res Commun

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The endoplasmic reticulum stress-inducible protein Niban regulates eIF2alpha and S6K1/4E-BP1 phosphorylation2007

    • Author(s)
      Sun GD, et. al.
    • Journal Title

      Biochem Biophys Res Commun. 360(1)

      Pages: 26-31

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Blood Tests for Asbestos-Related Mesothelioma2007

    • Author(s)
      Maeda M, et. al.
    • Journal Title

      Oncology 71(1-2)

      Pages: 181-7

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] GADD34 inhibits mammalian target of rapamycin signaling via tuberous sclerosis complex and controls cell survival under bioenergetic stress2007

    • Author(s)
      Watanabe R, et. al.
    • Journal Title

      Int J Mol Med. 19(3)

      Pages: 475-83

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] The endoplasmic reticulum stress-inducible protein Niban regulates eIF2alpha and S6K1/4E-BP1 phosphorylation2007

    • Author(s)
      Sun, GD, et. al.
    • Journal Title

      Biochem Biophys Res Commun 360(1)

      Pages: 181-7

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Blood Tests for Asbestos-Related Mesothelioma2007

    • Author(s)
      Maeda, M, et. al.
    • Journal Title

      Oncology 1-2

      Pages: 26-31

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] GADD34 inhibits mammalian target of rapamycin signaling via tuberous sclerosis complex and controls cell survival under bioenergetic stress2007

    • Author(s)
      Watanabe, R, et. al.
    • Journal Title

      Int J Mol Med 19(3)

      Pages: 475-83

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] The endoplasmic reticulum stress-inducible protein Niban regulates eIF2alpha and S6K1/4E-BPI phosphorylation2007

    • Author(s)
      Sun GD, et. al.
    • Journal Title

      Biochem Biophys Res Commun 360(1)

      Pages: 181-187

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Blood Tests for Asbestos-Related Mesothelioma.2007

    • Author(s)
      Maeda M, Hino O
    • Journal Title

      Oncology 71・1-2

      Pages: 26-31

    • Related Report
      2006 Annual Research Report
  • [Journal Article] GADD34 inhibits mammalian target of rapamycin signaling via tubero us sclerosis complex and controls cell survival under bioenergetic stress.2007

    • Author(s)
      Watanabe R, Tambe Y, Inoue H, Isono T, Haneda M, Isobe K, Kobayashi T, Hino O, et al.
    • Journal Title

      Int J Mol Med. 19・3

      Pages: 475-83

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Novel ELISA system for detection of N-ERC/mesothelin in the sera of mesothelioma patients2006

    • Author(s)
      Shiomi K, et. al.
    • Journal Title

      Cancer Sci. 97(9)

      Pages: 928-32

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Genetic dissection of vasculitis,myeloperoxidase-specific antineutrophil cytoplasmic autoantibody production,and related traits in spontaneous crescentic glomerulonephritis-forming/kinjoh mice2006

    • Author(s)
      Hamano Y, et. al.
    • Journal Title

      J Immunol. 176(6)

      Pages: 3662-73

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Interaction of Fox01 and TSC2 induces insulin resistance through activation of the mammalian target of rapamycin/p70 S6K pathway2006

    • Author(s)
      Cao Y, et. al.
    • Journal Title

      J Biol Chem. 281(52)

      Pages: 40242-51

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Novel ELISA system for detection of N-ERC/mesothelin in the sera of mesothelioma patients2006

    • Author(s)
      Shiomi, K, et. al.
    • Journal Title

      Cancer Sci 97(9)

      Pages: 928-32

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Genetic dissection of pasculitis, myeloperoxidase-specific antineutrophil cytoplasmic autoantibody production, and related traits in spontaneous crescentic glomerulonephritis-forming/kinjoh mice2006

    • Author(s)
      Hamano, Y, et. al.
    • Journal Title

      J Immunol 176(6)

      Pages: 3662-73

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Interaction of FoxO1 and TSC2 induces insulin resistance through activation of the mammalian target of rapamycin/p70 S6K pathway2006

    • Author(s)
      Cao, Y, et. al.
    • Journal Title

      J Biol Chem 281(52)

      Pages: 40242-51

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Genetic dissection of vasculitis, myeloperoxidase-specific antineurtophil cytoplasmic autoantibody production, and related traits in spontaneous crescentic glomerulonephritis-forming/kinjoh mice.2006

    • Author(s)
      Hamano Y, Tsukamoto K, Abe M, Sun G, Zhang D, et al.
    • Journal Title

      J Immunol. 176・6

      Pages: 3662-3673

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Novel ELISA system for detection on N-ERC/mesothelin in the sera of mesothelioma patients.2006

    • Author(s)
      Shiomi K, Miyamoto H, Segawa T, Higiwara Y, Hino O, et al.
    • Journal Title

      Cancer Sci. 97・9

      Pages: 928-932

    • NAID

      10018767738

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Interaction of FoxO1 and TSC2 induces insulin resistance through activation of the mammalian target of rapamycin/p70 S6K pathway.2006

    • Author(s)
      Cao Y, Kamioka Y, Yokoi N, Kobayashi T, Hino O, et al.
    • Journal Title

      J Biol Chem. 281・52

      Pages: 40242-51

    • Related Report
      2006 Annual Research Report
  • [Presentation] 遺伝性腎癌におけErc/Mesothelin遺伝子の機能解析2007

    • Author(s)
      小橋 丹青(張 丹青), ら
    • Organizer
      第30回日本分子生物学会年会、第80回日本生化学会大会 合同大会
    • Place of Presentation
      パシフィコ横浜 横浜
    • Year and Date
      2007-12-14
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] トランスジェニックEkerラットを用いたヒト結節性硬化症に関わるアミノ酸置換型Tsc2変異の解析2007

    • Author(s)
      塩野 雅俊, ら
    • Organizer
      第30回日本分子生物学会年会、第80回日本生化学会大会 合同大会
    • Place of Presentation
      パシフィコ横浜 横浜
    • Year and Date
      2007-12-14
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] The role of Erc/Mesothelin gene on renal carcinogenesis in Tsc2 knockout mice2007

    • Author(s)
      Zhang, D, et. al.
    • Organizer
      80th Biochemistry and 30th Molecular Biology 2007 Japan
    • Place of Presentation
      Pacifico Yokohama, Yokohama Japan
    • Year and Date
      2007-12-14
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Analysis of missense Tsc2 mutant associated with human tuberous sclerosis using a transgenic Eker rat system2007

    • Author(s)
      Shiono, M, et. al.
    • Organizer
      80th Biochemistry and 30th Molecular Biology 2007 Japan
    • Place of Presentation
      Pacifico Yokohama, Yokohama Japan
    • Year and Date
      2007-12-14
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 遺伝性腎癌におけるErc/Mesothelin遺伝子の機能解析2007

    • Author(s)
      小橋(張) 丹青, ら
    • Organizer
      第30回日本分子生物学会年会・80回日本学会大会合同大会
    • Place of Presentation
      パシフィコ横浜 横浜
    • Year and Date
      2007-12-14
    • Related Report
      2007 Annual Research Report
  • [Presentation] Tsc2遺伝子欠損腎癌移植系をモデルとしたsiRNAマイクロ粒子製剤化による新抗癌療法の試み2007

    • Author(s)
      今村 攻, ら
    • Organizer
      第66回 日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜 横浜
    • Year and Date
      2007-10-03
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Trial of new anti-cancer therapy by using siRNA microspheres in Tsc2 mutant renal carcinoma model2007

    • Author(s)
      Imamura, O, et. al.
    • Organizer
      66th Annual Meeting of the Japanese Cancer Association
    • Place of Presentation
      Pacifico Yokohama, Yokohama Japan
    • Year and Date
      2007-10-03
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Tsc2遺伝子欠損腎癌移植系をモデルとしたsiRNAマイクロ粒子製剤化による新抗癌療法の試み2007

    • Author(s)
      今村 攻, ら
    • Organizer
      第66回日本癌学会学術総会
    • Place of Presentation
      パシフィコ横浜 横浜
    • Year and Date
      2007-10-03
    • Related Report
      2007 Annual Research Report
  • [Presentation] Therapeutic Effect of Anti-MHC Class I mAb,RE2 on Hepatitis and Atopic Dermatitis2007

    • Author(s)
      Matsuoka S, et. al.
    • Organizer
      13th International Congress of Immunology
    • Place of Presentation
      Rio de Janeiro Brazil
    • Year and Date
      2007-08-21
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Therapeutic Effect of Anti-MHC Class I mAb, RE2 on Hepatitis and Atopic Dermatitis2007

    • Author(s)
      Matsuoka, S, et. al.
    • Organizer
      13th International Congress of Immunology
    • Place of Presentation
      Rio de Janeiro Brazil
    • Year and Date
      2007-08-21
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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