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Development of a feed-back controlled culture system to maximize calcification of tissue-engineered hone promoted by strain-induced fluid flow

Research Project

Project/Area Number 18500343
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionKanazawa University

Principal Investigator

TANAKA Shigeo  Kanazawa University, Institute of Nature and Environmental ental Technology, Associate Professor (20262602)

Co-Investigator(Kenkyū-buntansha) YAMAKOSHI Ken-ichi  Kanazawa University, Graduate School of Natural Sience and Technology, Professor (40014310)
KITAMURA Keiichiro  Kanazawa University, Graduate School of Medical Science, Associate Professor (80283117)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,020,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
KeywordsTissue engineering / Regenerative medicine / Bone / Osteoblast / Mechanical stimulation / Biomechanics / Cell / Calcification
Research Abstract

Stem cells have a potential to differentiate to specific organ cells and could be utilized to regenerate a lost organ with an appropriate scaffold in culture. This technology called tissue engineering or regenerative medicine is a promising strategy with a high biocompatibility avoiding the ethical problems in cadaveric donor transplantation and living donor transplantation Tissue-engineered bone composed of osteoblasts, a scaffold, and simulative hums was studied and developed in the past years, however it is still difficult to obtain sufficient calcification in vitro, comparing with natural bones. With this low mechano-compatibility, in particular a shortage of initial stiffness and toughness, tissue-engineered bone is not applicable to implantation to loaded bone sites. The purpose of this project is to development of a feed-back controlled culture system to maximize the calcification of tissue-engineered bone promoted by mechanical stimulation in fain of strain-induced fluid flow. This culture system is expected to provide better mechano-compatibility to an engineered bone before implantation. In this two-year project (2006-2007), two elemental technologies for the system were developed. In 2006, an optical device for non-destructive monitoring of in vitro calcification was developed using near-infrared light Using this device, the calcification of tissue-engineered bone composed of a type I collagen sponge and rat-primary cultured osteoblasts was monitored for 42 days in culture. In 3307, in the purpose of determination of initial condition of mechanical stimulation, a compact optical device was developed to observe concentration changes in intracellular calcium in osteoblasts populated in an engineered bone just after mechanical stimulation. These achievements should contribute to completion of the feed-hack controlled culture system for tissue-engineered bone.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (25 results)

All 2008 2007 2006

All Journal Article (7 results) (of which Peer Reviewed: 4 results) Presentation (15 results) Patent(Industrial Property Rights) (3 results)

  • [Journal Article] 光を用いた再生骨の石灰化モニタリング2008

    • Author(s)
      垣尾雅文、杉浦直樹、山越憲一、田中茂雄
    • Journal Title

      日本臨床バイオメカニクス学会誌 (印刷予定)

    • NAID

      10025631167

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Non-destructive optical monitoring for calcification of tissue-engineered bone in vitro2008

    • Author(s)
      Shigeo M. Tanaka, Masafumi Kakio, Ken-ichi Yamakoshi
    • Journal Title

      Journal of Biomechanical Science and Engineering (印刷予定)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Optical monitoring of in vitro calcification in tissue-engineered bone2008

    • Author(s)
      Masafumi, Kakio, Naoki, Sugiura, Ken-ichi, Yamakoshi, Shigeo, M., Tanaka
    • Journal Title

      Japanese Journal of Clinical Biomechanics

    • NAID

      10025631167

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Non-destructive optical monitoring for calcification of tissue-engineered bone in vitro2008

    • Author(s)
      Shigeo, M., Tanaka, Masafumi, Kakio, Ken-ichi, Yamakoshi
    • Journal Title

      Journal of Biomechanical Science and Engineering

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 光を用いた再生骨の石灰化モニタリング2008

    • Author(s)
      垣尾 雅文, 杉浦 直樹, 山越 憲一, 田中 茂雄
    • Journal Title

      日本臨床バイオメカニクス学会誌 (掲載確定)

    • NAID

      10025631167

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Non-destructive optical monitoring for calcification of tissue-engineered bone in vitro2008

    • Author(s)
      Shigeo M. Tanaka, Masafumi Kakio, Ken-ichi Yamakoshi
    • Journal Title

      Journal of Biomechanical Science and Engineering (掲載確定)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 近赤外光を用いた培養骨芽細胞の石灰化モニタリングシステムの開発2007

    • Author(s)
      垣尾雅文, 野川雅道, 田中志信, 山越憲一, 田中茂雄
    • Journal Title

      日本機械学会北信越学生会第36回学生員卒業研究発表講演会講演論文集

      Pages: 185-186

    • NAID

      120001132814

    • Related Report
      2006 Annual Research Report
  • [Presentation] 小型光学素子を用いた細胞内Ca^<2+>動態観察システム2008

    • Author(s)
      杉浦直樹、垣尾雅文、山越憲一、田中茂雄
    • Organizer
      日本機械学会北信越学生会第37回学生員卒業研究発表講演会
    • Place of Presentation
      福井工業大学
    • Year and Date
      2008-03-07
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] An observation system for intracellular Ca2+ dynamics using a compact optical device2008

    • Author(s)
      Naoki, Sugiura, Masafumi, Kakio, Kenichi, Yamakoshi, Shigeo, Tanaka
    • Organizer
      The 37th Graduate Student Conference, The Japan Society of Mechanical Engineering Hokuriku-Shinetsu Branch
    • Place of Presentation
      Ishikawa
    • Year and Date
      2008-03-07
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 小型光学素子を用いた細胞内Ca2+動態観察システム2008

    • Author(s)
      杉浦 直樹, 垣尾 雅文, 山越 憲一, 田中 茂雄
    • Organizer
      日本機械学会北信越学生会第37回学生員卒業研究発表講演会
    • Place of Presentation
      福井工業大学
    • Year and Date
      2008-03-07
    • Related Report
      2007 Annual Research Report
  • [Presentation] 光を用いた再生骨の非破壊的石灰化モニタリング2008

    • Author(s)
      垣尾雅文、山越憲一、田中茂雄
    • Organizer
      平成19年度日本生体医工学会北陸支部大会
    • Place of Presentation
      金沢大学
    • Year and Date
      2008-01-26
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Non-destructive monitoring for in vitro calcification of tissue-engineered bone using near-infrared light2008

    • Author(s)
      Masafumi, Kakio, Kenichi, Yamakoshi, Shigeo, Tanaka
    • Organizer
      2007 Annual Meeting of Hokuriku Branch, Japanese Society Medical and Biological Engineering
    • Place of Presentation
      Kanazawa
    • Year and Date
      2008-01-26
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 再生骨の光センシング2008

    • Author(s)
      田中茂雄、垣尾雅文、杉浦直樹、山越憲一
    • Organizer
      第20回バイオエンジニアリング講演会
    • Place of Presentation
      芝浦工業大学
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Optical sensing in tissue-engineered bone2008

    • Author(s)
      Shigeo, Tanaka, Masahumi, Kakio, Naoki, Sugiura, Kenichi, Yamakoshi
    • Organizer
      The 20th Bioengineering Conference, 2007 Annual Meeting of BED/JSME
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 近赤外光を用いた培養骨芽細胞の石灰化モニタリングシステムの開発2007

    • Author(s)
      垣尾雅文、野川雅道、田中志信、山越憲一、田中茂雄
    • Organizer
      日本機械学会北信越学生会第36回学生員卒業研究発表講演会
    • Place of Presentation
      石川工業高等専門学校
    • Year and Date
      2007-03-07
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of optical monitoring System of osteoblastic calcification in vitro using near-infrared light2007

    • Author(s)
      Masafumi, Kakio, Masamichi, Nogawa, Shinobu, Tanaka, Kenichi, Yamakoshi, Shigeo, Tanaka
    • Organizer
      The 36th Graduate Student Conference, The Japan Society of Mechanical Engineering Hokuriku-Shinetsu Branch
    • Place of Presentation
      Ishikawa
    • Year and Date
      2007-03-07
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 近赤外強度分解法による再生骨の石灰化モニタリング2007

    • Author(s)
      垣尾雅文、山越憲一、田中茂雄
    • Organizer
      第46回日本生体医工学大会
    • Place of Presentation
      仙台国際センター
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] An optical monitoring system for in vitro osteogenesis2007

    • Author(s)
      S. M. Tanaka, M. Kakio, K. Yamakoshi
    • Organizer
      Third Asian Pacific Conference on Biomechanics
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] 光を用いた再生骨の石灰化モニタリング2007

    • Author(s)
      垣尾雅文、杉浦直樹、山越憲一、田中茂雄
    • Organizer
      第34回日本臨床バイオメカニクス学会
    • Place of Presentation
      早稲田大学国際会議場
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] A monitoring system for in vitro osteogenesis using intensity-resolved Near-infrared light2007

    • Author(s)
      Masafumi, Kakio, Kenichi, Yamakoshi, Shigeo, Tanaka
    • Organizer
      The 46th Annual Conference of Japanese Society Medical and Biological Engineering
    • Place of Presentation
      Sendai
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] An optical monitoring system for in vitro osteogenesis2007

    • Author(s)
      Shigeo, M., Tanaka, Masafumi, Kakio, Kenichi, Yamakoshi
    • Organizer
      Third Asian Pacific Conference on Biomechanic
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] In vitro optical monitoring of calcification in tissue-engineered bone2007

    • Author(s)
      Masafumi, Kakio, Naoki, Sugiura, Kenichi, Yamakoshi, Shigeo, Tanaka
    • Organizer
      The 34th Annual Meeting of the Japanese for Clinical Biomechanics
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 再生組織用細胞内カルシウムイオンモニタリング装置2008

    • Inventor(s)
      田中茂雄
    • Industrial Property Rights Holder
      金沢大学
    • Industrial Property Number
      2008-055393
    • Filing Date
      2008-03-05
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Patent(Industrial Property Rights)] 再生組織用細胞内カルシウムイオンモニタリング装置2008

    • Inventor(s)
      田中 茂雄
    • Industrial Property Rights Holder
      金沢大学
    • Filing Date
      2008-03-05
    • Related Report
      2007 Annual Research Report
  • [Patent(Industrial Property Rights)] 骨密度計測装置およびその方法2006

    • Inventor(s)
      田中茂雄、野川雅道
    • Industrial Property Rights Holder
      金沢大学
    • Industrial Property Number
      2006-319343
    • Filing Date
      2006-11-27
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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