| Project/Area Number |
18500352
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Meiji University |
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Principal Investigator |
HIRAOKA Wakako Meiji University, School of Science and Technology, Associate Professor (00212168)
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| Co-Investigator(Kenkyū-buntansha) |
CHOI Pak-kon Meiji University, School of Science and Technology, Professor (30143530)
KONDO Takashi University of Toyama, Faculty of Medicine, Professor (40143937)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,870,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2006: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | Biophysics / ultrasound / fluorescent dye / sonodynamic reagent / cell culture / sonodynamic therapy / caged drug / hydrophone |
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| Research Abstract |
We have tried to develop an ultrasound spot-irradiation system with real-time optical monitoring for biological applications. Ultrasound has been used for various physical and chemical purposes for long time. In addition, therapeutic application of ultrasound has been a focus of constant attention recently. In this study, we made an ultrasound spot-irradiation system in which irradiated cells or tissues were monitored under microscopic stage. This system also equipped with micromanipulation and injection for microsurgery of cells and tissues. As a apparatus of ultrasound, we applied several types of piezzoceramic focusing of transducer for the range from 1 MHz to 7 MHz. These apparatus was designed for both continuous and burst wave. The power of ultrasound was determined by calorimetry.
For the development of new application with this system, we surveyed several kinds of photo-activable caged reagents for sonodynamic therapy. As caged reagents, we employed 5-carboxymethoxy-2-nitrobenzy
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l(CMNB)-caged fluorescein, Dextran,((5-carboxyraethoxy-2-nitrobenzyl) oxy) carbonyl-caged-5-(and-6)-carboxy-Q-rhodamine, and CMNB-caged carboxy fluorescein. To access the cleavage of caged crosslinkage, the increase of fluorescence was determined after sonolysis or photolysis of drugs. After the sonolysis of the reagents at a concentration of 1 μM in PBS, the intensity of fluorescence of solution increased in time dependence . Activation of drugs within the cultured cells was assayed with flow cytometer(Cytomks FC 500, Beckman-Coulter USA). PLB-985 cells(human myelomonoblastic leukemia cells derived from B-cell) were used for this assay. The cells were incubated with 10 PA CMNB-caged fluorescein in PPS for 10 minutes at 37℃. Cytometric analysis of sonicated cells showed a partial activation of CMNB-caged fluorescein within the cells. These results suggest the possibility of the device of new caged compounds using ultrasound, which are also expected as tracers and therapeutic reagents. Our system will open the possibility of new application of sonotherapy. Less
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