Development of a new method for transfection to the skin by ultrasound

• Project/Area Number: 18500391
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Medical systems
• Research Institution: Fukuoka University
• Principal Investigator: IRIE Yutaka Fukuoka University, 医学部, 助教 (80352235)
• Co-Investigator(Kenkyū-buntansha): TACHIBANA Katsuro 福岡大学, 医学部, 教授 (40271605) ; OGAWA Koichi 福岡大学, 医学部, 准教授 (60078780)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,910,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥510,000); Fiscal Year 2008: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
• Keywords: 超音波照射 / 癌細胞皮膚

Research Abstract

8週齢の雌のヘアレスマウスの背部にマイクロバブルと遺伝子の混合溶液0.05mlを複数箇所皮内投与した(SonoVue、Bracco社製、GFP(1μg/ml))。超音波発振素子画径6mmのプローベを用いて麻酔下の皮膚表面から照射した(2143kHz, 50% duty cycle, 3Hz Burst rate)。超音波照射後48時間後観察で、超音波強度0.5W/cm2、照射時間2と3minの群でGFPの発現が認められた。

Research Products

• [Journal Article] Evaluation and comparison of three novel microbubbles: Enhancement of ultrasound-induced cell death and free radicals production (2009)
  • Author(s): Hassan MA, Feril LB Jr, Suzuki K, Kudo N, Tachibana K, Kondo
  • Journal Title: Ultrason Sonochem 16(3) Pages: 372-378
  • Peer Reviewed
• [Journal Article] Gene Transfection through microbubble-aided sonication in cancer cells (2009)
  • Author(s): Dickerson C. Moreno, Feril LB Jr, Tachibana K, Irie Y
  • Journal Title: Philippine Physics Journal 31(3) Pages: 1-6
  • Peer Reviewed
• [Journal Article] Growth inhibition of neurofibroma by ultrasound-mediated interferon γtransfection (2009)
  • Author(s): Yamaguchi K, Feril LB Jr, Harada Y, Endo H, Irie Y, Nakayama J, Tachibana K
  • Journal Title: Journal of Medical Ultrasonics 36 Pages: 3-8
  • NAID: 10025327746
  • Peer Reviewed
• [Journal Article] Growth inhibition of neurofibroma by ultrasound-mediated interferon γ transfection (2009)
  • Author(s): Yamaguchi K
  • Journal Title: J. Med Ultrasonics 36 Pages: 3-8
  • Peer Reviewed
• [Journal Article] Gene Transfection through microbubble-aided sonication in cancer cells (2009)
  • Author(s): Dickerson C. Moreno
  • Journal Title: Philippine Physics Journal 31 Pages: 1-7
  • Peer Reviewed
• [Journal Article] Therapeutic potentials of low-intensity ultrasound (part II) : Biomolecular effects, sono transfection and sono permeabilization (2008)
  • Author(s): Tachibana K, Ogawa K, Ikeda-Dantsuji Y, Endo H, Harada Y, Kondo T, Ogawa R
  • Journal Title: J. Med Ultrasonics 35 Pages: 161-167
  • Peer Reviewed
• [Journal Article] Therapeutic Potential of Low-Intensity Ultrasound (Part1) : Thermal and Sonomechanical Effects (2008)
  • Author(s): FERIL Loreto
  • Journal Title: J. Med Ultrasonics 35 Pages: 153-160
  • Peer Reviewed
• [Journal Article] Bioeffects of Ultrasound for Therapy (2007)
  • Author(s): Feril LB Jr, Tachibana K, Yamaguchi K,Ogawa K, Ikeda-Dantsuji Y, Irie Y and Endo H
  • Journal Title: Philippine Physics Journal 29 Pages: 1-4
  • Peer Reviewed
• [Journal Article] 超音波の治療への応用 (2006)
  • Author(s): 立花克郎, 入江 豊, 小川皓一
  • Journal Title: J Med Ultrasound 33(6) Pages: 631-639
  • NAID: 10020611604
• [Presentation] マイクロバブルの治療への応用 (2008)
  • Author(s): 立花克郎
  • Organizer: 第10回国際造影超音波シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 2008-12-13
• [Presentation] 超音波遺伝子導入ブースター剤の開発 (2008)
  • Author(s): 立花克郎
  • Organizer: 第10回福岡大学技術交流会産学官連携グランドコンベンション
  • Place of Presentation: 福岡
  • Year and Date: 2008-12-04
• [Presentation] Sonoporation法による皮膚への遺伝子導入 (2008)
  • Author(s): 入江豊, 立花克郎, フェリル ロリト, 遠藤日富美, 山口和記, 原田慶美
  • Organizer: 日本超音波学会第81回学術集会
  • Place of Presentation: 神戸
  • Year and Date: 2008-05-23
• [Presentation] 超音波の治療への応用-再生医療から癌治療まで- (2007)
  • Author(s): 立花克郎
  • Organizer: 日本皮膚科学会第340回福岡地方会
  • Place of Presentation: 福岡市
  • Year and Date: 2007-03-10
• [Presentation] Interferonβ Sonotransfection Inhibits Proliferation of Malignant Melanoma In-vivo (2007)
  • Author(s): Yamaguchi K, Feril LB. Jr., Takahashi A, Harada Y, Ogawa K, Irie Y, Tachibana K, Nakayama J
  • Organizer: 7th international Symposium on Therapeutic Ultrasound
  • Place of Presentation: Seoul Korea
• [Remarks]
  • URL: http://resweb2.jhk.adm.fukuoka-u.ac.jp/FukuokaUniv/R101J_Action.do