Age-related differences in the effect of weight bearing after disuse atrophy in dynamics of muscle cell nucleus.
| Project/Area Number |
18500398
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Rehabilitation science/Welfare engineering
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| Research Institution | Kanazawa University |
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Principal Investigator |
YOKONAWA Masami Kanazawa University, Graduate School of Medicine, Assistant Professor (80303288)
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| Co-Investigator(Kenkyū-buntansha) |
YAMAZAKI Toshiaki Kanazawa University, Graduate School of Medicine, Professor (00220319)
TACHINO Katsuhiko Kanazawa University, Graduate School of Medicine, Professor (40092788)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,890,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | rehabilitation / aging / histochemistry |
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| Research Abstract |
In the skeletal muscle of the elderly there is a decrease in the muscle mass as well as a decline in muscle strength. The atrophying process in the absence of activity in the elderly may differ from that in the young due to age-associated changes in their skeletal muscles. It is necessary for physiotherapy to review an adequate method by the elderly during an inactivity period. In this study, nuclear kinetics and histochemical analyses of hindlimb muscle of each rat were conducted to determine the effect of weight healing (WB) on disuse atrophy would differ with advancing age.
3-, 7-, 14-month-old male rats were assigned to two experimental group and one control group for a 3-week experiment. HS group had their hindlimb kept in suspension. HS+ group were also suspended but subjected to loading of the hindlimb muscle through WE hr one hour per day that began one week after the commencement of the experiment The control group was subjected to no suspension. We obtained hindlimb muscle after experiment termination and made cryosection.
We compared increase in planting activity of a satellite cell with control group at 3-month-old and decreased in HS group, HS+ group. It decreased at 7-month-old and 14-month-old. The proliferative activity of apoptosis nuclei increased with age. However, there was no marked difference between HS and HS+ groups.
Muscle fiber cross-sectional area (MCSA) of HS group and HS+ group decreased than control group regardless of age. WB was relatively effective at 7-month-old. At 3-month-old, there was no marked difference between HS and HS+ groups. In type I fibers at 14-month-old, MCSA in HS+ group was slightly lower than that in the HS group. The response to own-weight loading depended on age.
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Report
(3 results)
Research Products
(6 results)
