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Effect of dynamic compressive loading on three-dimensional scaffold-embedded chondrocytes

Research Project

Project/Area Number 18500400
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Rehabilitation science/Welfare engineering
Research InstitutionShiga University of Medical Science

Principal Investigator

IMAI Shinji  Shiga University of Medical Science, 医学部, 准教授 (90283556)

Co-Investigator(Kenkyū-buntansha) MATSUSUE Yoshitaka  滋賀医科大学, 医学部, 教授 (30209548)
Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥4,020,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥720,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
Keywords力学的刺激 / 関節軟骨 / 軟骨細胞 / 3次元培養 / コラーゲン
Research Abstract

軟骨細胞は細胞周囲の環境に応じて、その活性を調節している. 軟骨細胞の脱分化を抑制すべく周期的機械的刺戟を加え、脱分化軟骨細胞の賦活化を試みた. 3次元包埋下圧縮ストレスによってI型コラーゲンの発現が減少し、II型コラーゲンおよびアグリカンの発現が増加し、軟骨細胞の再分化が促されることが明らかになった. BMP-2、b-FGFと機械的刺戟を同時に加えることによる相乗効果を期待したが、逆に効果が弱くなる事が明らかになった.

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (29 results)

All 2008 2007 2006

All Journal Article (12 results) (of which Peer Reviewed: 10 results) Presentation (13 results) Book (4 results)

  • [Journal Article] Intrasynovial lipoma causing trigger wrist and carpal tunnel syndrome2008

    • Author(s)
      Imai S, Kodama N, Matsusue Y.
    • Journal Title

      Scand J Plastic Recons 42

      Pages: 328-330

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Closed rupture of a flexor tendon of unknown aetiology2008

    • Author(s)
      Imai S, Andou K, Kodama N, Isoya E, Okabe H, Matsusue Y.
    • Journal Title

      J Hand Surg 33B

      Pages: 90-91

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] A novel exogenous concentration-gradient collagen scaffold augments full-thickness articular cartilage repair2008

    • Author(s)
      Mimura T, Imai S, Kubo M, Isoya E, Ando K, Matsusue Y.
    • Journal Title

      Osteoarthritis Cartilage 16

      Pages: 1083-1091

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Intrasynovial lipoma causing trigger wrist and carpal tunnel syndrome.2008

    • Author(s)
      Imai S, Kodama N, Matsusue Y
    • Journal Title

      Scand J Plastic Recons. 42

      Pages: 328-330

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Closed rupture of a flexor tendon of unknown aetiology.2008

    • Author(s)
      Imai S, Andou K, Kodama N, Isoya E, Okabe H, Matsusue Y.
    • Journal Title

      J Hand Sure (British Edition) 33

      Pages: 90-91

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] A novel exogenous concentration-gradient collagen scaffold augments full-thickness articular cartilage repair.2008

    • Author(s)
      Mimura T, Imai S, Kubo M, Isoya E, Ando K, Matsusue.
    • Journal Title

      Osteoarthritis Cartilage 16

      Pages: 1083-1091

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Congenial unilateral upper limb muscular hypertrophy associated with contracture of the extrinsic extensor tendon2007

    • Author(s)
      Imai S, Isoya E, Kubo M, Kodama N, Matsusue Y.
    • Journal Title

      J Hand Surg 32B

      Pages: 308-310

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] 骨粗鬆症性脊椎骨折の整復に置ける椎体形成術の有効性2007

    • Author(s)
      今井晋二, 菊地克久, 猿橋康雄, 松末吉隆
    • Journal Title

      中部整災誌 50

      Pages: 81-82

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Exogenous Collagen Augments Repair of Full-thickness Cartilage Defect by Facilitating Mesenchymal Cell Recruitment2007

    • Author(s)
      Kubo M, Imai S, Fujimiya M, Isoya E, Ando K, Matsusue Y.
    • Journal Title

      Detailed Cellular Events during Cartilage Repair- Acta Orthop 78

      Pages: 845-55

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Congenial unilateral upper limb muscular hypertrophy associated with contracture of the extrinsic extensor tendon2007

    • Author(s)
      Imai S, Isoya E, Kubo M, Kodama N, Matsusue Y
    • Journal Title

      J Hand Surg (British Edition) 32

      Pages: 3008-310

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 骨粗懸症性脊椎骨折の整復に置ける椎体形成術の有効性2007

    • Author(s)
      今井 晋二, 菊地 克久, 猿橋 康雄, 松末 吉隆
    • Journal Title

      中部整災誌 50

      Pages: 81-82

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Exogenous Collagen Augments Repair of Full-thiclrness Cartilage Defect by Facilitating Mesenchymal Cell Recmitment.-Detailed Cellular Events during Cartilage Repair-2007

    • Author(s)
      Kubo M, Imai S, Fujimiya M, Isoya E, Ando K, Matsusue Y
    • Journal Title

      Acta Orthop 78

      Pages: 845-855

    • Related Report
      2007 Annual Research Report
  • [Presentation] 肩関節鏡視下腱板修復術と広範囲腱板断裂の問題点2008

    • Author(s)
      今井晋二
    • Organizer
      滋賀県臨床整形外科学会
    • Place of Presentation
      大津市
    • Year and Date
      2008-10-04
    • Related Report
      2008 Annual Research Report
  • [Presentation] Combination of collagen scaffold and timely release of bone morphogenetic protein-2 enhances the repair of full-thickness defect of articular cartilage2008

    • Author(s)
      Mimura T, Imai S, Nishizawa K, Okumura N, Ando K, Kubo M, Matsusue Y.
    • Organizer
      OARSI World Congress on Osteoarthritis 2008
    • Place of Presentation
      Rome, Italy
    • Year and Date
      2008-09-19
    • Related Report
      2008 Final Research Report
  • [Presentation] The role of the volume-sensitive Cl-current in the process of regulatory volume decrease (RVD) in freshly isolated articular chondrocytes2008

    • Author(s)
      Okumura N, Toyota F, Isoya E, Imai S,Matsuura H, Matsusue Y.
    • Organizer
      OARSI World Congress on Osteoarthritis 2008
    • Place of Presentation
      Rome, Italy
    • Year and Date
      2008-09-19
    • Related Report
      2008 Final Research Report
  • [Presentation] The effect of low-intensity pulsed ultrasound for formaitn of scaffold-free cartilage2008

    • Author(s)
      Uenaka K, Imai S, Andou K, Matsusue Y.
    • Organizer
      OARSI World Congress on Osteoarthritis 2008
    • Place of Presentation
      Rome, Italy
    • Year and Date
      2008-09-19
    • Related Report
      2008 Final Research Report
  • [Presentation] In-advance transmedullary stimulation of mesenchymal stem cells enhances spontaneous repair of full-thickness articular cartilage defect2008

    • Author(s)
      Nishizawa K, Imai S,Mimura T, Kubo M, Matsusue Y.
    • Organizer
      OARSI World Congress on Osteoarthritis 2008
    • Place of Presentation
      Rome, Italy
    • Year and Date
      2008-09-19
    • Related Report
      2008 Final Research Report
  • [Presentation] Mechanical stress-induced chronological phenotypic changes of chondrocytes in three-dimentional scaffold2008

    • Author(s)
      Ando K, Imai S, Shioji S, Mimura T, Okumura N, Uenaka K, Kasahara T, Nishizawa K, Kubo M, Matsusue Y.
    • Organizer
      OARSI World Congress on Osteoarthritis 2008
    • Place of Presentation
      Rome, Italy
    • Year and Date
      2008-09-19
    • Related Report
      2008 Final Research Report
  • [Presentation] Diabetes impairs fracture healing by repressing the osteoclast function2008

    • Author(s)
      Kasahara T, Imai S, Matsusue Y, Fujimiya M, Kojima H.
    • Organizer
      OARSI World Congress on Osteoarthritis 2008
    • Place of Presentation
      Rome, Italy
    • Year and Date
      2008-09-19
    • Related Report
      2008 Final Research Report
  • [Presentation] Mechanical stress modulates chondrocytes phenotype via IL-42008

    • Author(s)
      Shioji S, Imai S, Ando K, Mimura T, Okumura N, Matsusue Y.
    • Organizer
      OARSI World Congress on Osteoarthritis 2008
    • Place of Presentation
      Rome, Italy
    • Year and Date
      2008-09-19
    • Related Report
      2008 Final Research Report
  • [Presentation] Effect of dynamic compressive loading in combination with growth factor on the cartilage-specific gene expression of 3D scaffold-embedded chondrocytes2007

    • Author(s)
      Ando K, Imai S, Isoya E, Kubo M, Okumura N, Mimura T, Matsusue Y.
    • Organizer
      OARSI World Congress on Osteoarthritis 2007
    • Place of Presentation
      Fort Lauderdale, Florida, USA
    • Year and Date
      2007-12-07
    • Related Report
      2008 Final Research Report
  • [Presentation] A novel concentration-gradient collagen scaffold augments due2007

    • Author(s)
      Mimura T, Imai S, Kubo M, Isoya E, Okumura N, Ando K, Matsusue Y.
    • Organizer
      OARSI World Congress on Osteoarthritis 2007
    • Place of Presentation
      Fort Lauderdale, Florida, USA
    • Year and Date
      2007-12-07
    • Related Report
      2008 Final Research Report
  • [Presentation] 高解像度3D・CTを用いた骨粗霧症患者腰椎の骨梁構造解析2007

    • Author(s)
      今井 晋二
    • Organizer
      第44回日本リハビリテーション医学会学術集会
    • Place of Presentation
      神戸市
    • Year and Date
      2007-06-08
    • Related Report
      2007 Annual Research Report
  • [Presentation] Swelling-activated chloride current and its inhibition by arachidonic acid in rabbit articular chondrocytes2006

    • Author(s)
      Isoya E, Matsuura H, Toyoda F, Imai S, Kubo M, Matsusue Y.
    • Organizer
      International Cartilage Repair Society 6th Symposium
    • Place of Presentation
      San Diego, CA, USA
    • Year and Date
      2006-01-08
    • Related Report
      2008 Final Research Report
  • [Presentation] Collagen scaffold augments large full-thickness articular cartilage repair by active mesenchymal cell recruitment. -detaild cellular events during cartilage repair-2006

    • Author(s)
      Kubo M, Imai S, Isoya E, Ando K, Matsusue Y.
    • Organizer
      International Cartilage Repair Society 6th Symposium
    • Place of Presentation
      San Diego, CA, USA
    • Year and Date
      2006-01-08
    • Related Report
      2008 Final Research Report
  • [Book] 関節軟骨:最新整形外科学大系1.運動器の生物学と生体力学(越智隆弘監修)2008

    • Author(s)
      今井晋二、松末吉隆
    • Publisher
      東京, 中山書店
    • Related Report
      2008 Final Research Report
  • [Book] 関節軟骨 : 最新整形外科学大系1(越智隆弘監修)2008

    • Author(s)
      今井晋二, 松末吉隆
    • Total Pages
      6
    • Publisher
      中山書店
    • Related Report
      2008 Annual Research Report
  • [Book] 神経による骨形成の調節について:リウマチ病セミナーXVIII(七川歓次監修)2007

    • Author(s)
      今井晋二
    • Publisher
      大阪, 長井書店
    • Related Report
      2008 Final Research Report
  • [Book] リウマチ病セミナーXVIII(七川歓次監修)2007

    • Author(s)
      今井 晋二
    • Publisher
      神経による骨形成の調節について
    • Related Report
      2007 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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