Effect of dynamic compressive loading on three-dimensional scaffold-embedded chondrocytes

• Project/Area Number: 18500400
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Rehabilitation science/Welfare engineering
• Research Institution: Shiga University of Medical Science
• Principal Investigator: IMAI Shinji Shiga University of Medical Science, 医学部, 准教授 (90283556)
• Co-Investigator(Kenkyū-buntansha): MATSUSUE Yoshitaka 滋賀医科大学, 医学部, 教授 (30209548)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,020,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥720,000); Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
• Keywords: 力学的刺激 / 関節軟骨 / 軟骨細胞 / 3次元培養 / コラーゲン

Research Abstract

軟骨細胞は細胞周囲の環境に応じて、その活性を調節している. 軟骨細胞の脱分化を抑制すべく周期的機械的刺戟を加え、脱分化軟骨細胞の賦活化を試みた. 3次元包埋下圧縮ストレスによってI型コラーゲンの発現が減少し、II型コラーゲンおよびアグリカンの発現が増加し、軟骨細胞の再分化が促されることが明らかになった. BMP-2、b-FGFと機械的刺戟を同時に加えることによる相乗効果を期待したが、逆に効果が弱くなる事が明らかになった.

Research Products

• [Journal Article] Intrasynovial lipoma causing trigger wrist and carpal tunnel syndrome (2008)
  • Author(s): Imai S, Kodama N, Matsusue Y.
  • Journal Title: Scand J Plastic Recons 42 Pages: 328-330
  • Peer Reviewed
• [Journal Article] Closed rupture of a flexor tendon of unknown aetiology (2008)
  • Author(s): Imai S, Andou K, Kodama N, Isoya E, Okabe H, Matsusue Y.
  • Journal Title: J Hand Surg 33B Pages: 90-91
  • Peer Reviewed
• [Journal Article] A novel exogenous concentration-gradient collagen scaffold augments full-thickness articular cartilage repair (2008)
  • Author(s): Mimura T, Imai S, Kubo M, Isoya E, Ando K, Matsusue Y.
  • Journal Title: Osteoarthritis Cartilage 16 Pages: 1083-1091
  • Peer Reviewed
• [Journal Article] Intrasynovial lipoma causing trigger wrist and carpal tunnel syndrome. (2008)
  • Author(s): Imai S, Kodama N, Matsusue Y
  • Journal Title: Scand J Plastic Recons. 42 Pages: 328-330
  • Peer Reviewed
• [Journal Article] Closed rupture of a flexor tendon of unknown aetiology. (2008)
  • Author(s): Imai S, Andou K, Kodama N, Isoya E, Okabe H, Matsusue Y.
  • Journal Title: J Hand Sure (British Edition) 33 Pages: 90-91
  • Peer Reviewed
• [Journal Article] A novel exogenous concentration-gradient collagen scaffold augments full-thickness articular cartilage repair. (2008)
  • Author(s): Mimura T, Imai S, Kubo M, Isoya E, Ando K, Matsusue.
  • Journal Title: Osteoarthritis Cartilage 16 Pages: 1083-1091
  • Peer Reviewed
• [Journal Article] Congenial unilateral upper limb muscular hypertrophy associated with contracture of the extrinsic extensor tendon (2007)
  • Author(s): Imai S, Isoya E, Kubo M, Kodama N, Matsusue Y.
  • Journal Title: J Hand Surg 32B Pages: 308-310
  • Peer Reviewed
• [Journal Article] 骨粗鬆症性脊椎骨折の整復に置ける椎体形成術の有効性 (2007)
  • Author(s): 今井晋二, 菊地克久, 猿橋康雄, 松末吉隆
  • Journal Title: 中部整災誌 50 Pages: 81-82
  • Peer Reviewed
• [Journal Article] Exogenous Collagen Augments Repair of Full-thickness Cartilage Defect by Facilitating Mesenchymal Cell Recruitment (2007)
  • Author(s): Kubo M, Imai S, Fujimiya M, Isoya E, Ando K, Matsusue Y.
  • Journal Title: Detailed Cellular Events during Cartilage Repair- Acta Orthop 78 Pages: 845-55
  • Peer Reviewed
• [Journal Article] Congenial unilateral upper limb muscular hypertrophy associated with contracture of the extrinsic extensor tendon (2007)
  • Author(s): Imai S, Isoya E, Kubo M, Kodama N, Matsusue Y
  • Journal Title: J Hand Surg (British Edition) 32 Pages: 3008-310
  • Peer Reviewed
• [Journal Article] 骨粗懸症性脊椎骨折の整復に置ける椎体形成術の有効性 (2007)
  • Author(s): 今井 晋二, 菊地 克久, 猿橋 康雄, 松末 吉隆
  • Journal Title: 中部整災誌 50 Pages: 81-82
• [Journal Article] Exogenous Collagen Augments Repair of Full-thiclrness Cartilage Defect by Facilitating Mesenchymal Cell Recmitment.-Detailed Cellular Events during Cartilage Repair- (2007)
  • Author(s): Kubo M, Imai S, Fujimiya M, Isoya E, Ando K, Matsusue Y
  • Journal Title: Acta Orthop 78 Pages: 845-855
• [Presentation] 肩関節鏡視下腱板修復術と広範囲腱板断裂の問題点 (2008)
  • Author(s): 今井晋二
  • Organizer: 滋賀県臨床整形外科学会
  • Place of Presentation: 大津市
  • Year and Date: 2008-10-04
• [Presentation] Combination of collagen scaffold and timely release of bone morphogenetic protein-2 enhances the repair of full-thickness defect of articular cartilage (2008)
  • Author(s): Mimura T, Imai S, Nishizawa K, Okumura N, Ando K, Kubo M, Matsusue Y.
  • Organizer: OARSI World Congress on Osteoarthritis 2008
  • Place of Presentation: Rome, Italy
  • Year and Date: 2008-09-19
• [Presentation] The role of the volume-sensitive Cl-current in the process of regulatory volume decrease (RVD) in freshly isolated articular chondrocytes (2008)
  • Author(s): Okumura N, Toyota F, Isoya E, Imai S,Matsuura H, Matsusue Y.
  • Organizer: OARSI World Congress on Osteoarthritis 2008
  • Place of Presentation: Rome, Italy
  • Year and Date: 2008-09-19
• [Presentation] The effect of low-intensity pulsed ultrasound for formaitn of scaffold-free cartilage (2008)
  • Author(s): Uenaka K, Imai S, Andou K, Matsusue Y.
  • Organizer: OARSI World Congress on Osteoarthritis 2008
  • Place of Presentation: Rome, Italy
  • Year and Date: 2008-09-19
• [Presentation] In-advance transmedullary stimulation of mesenchymal stem cells enhances spontaneous repair of full-thickness articular cartilage defect (2008)
  • Author(s): Nishizawa K, Imai S,Mimura T, Kubo M, Matsusue Y.
  • Organizer: OARSI World Congress on Osteoarthritis 2008
  • Place of Presentation: Rome, Italy
  • Year and Date: 2008-09-19
• [Presentation] Mechanical stress-induced chronological phenotypic changes of chondrocytes in three-dimentional scaffold (2008)
  • Author(s): Ando K, Imai S, Shioji S, Mimura T, Okumura N, Uenaka K, Kasahara T, Nishizawa K, Kubo M, Matsusue Y.
  • Organizer: OARSI World Congress on Osteoarthritis 2008
  • Place of Presentation: Rome, Italy
  • Year and Date: 2008-09-19
• [Presentation] Diabetes impairs fracture healing by repressing the osteoclast function (2008)
  • Author(s): Kasahara T, Imai S, Matsusue Y, Fujimiya M, Kojima H.
  • Organizer: OARSI World Congress on Osteoarthritis 2008
  • Place of Presentation: Rome, Italy
  • Year and Date: 2008-09-19
• [Presentation] Mechanical stress modulates chondrocytes phenotype via IL-4 (2008)
  • Author(s): Shioji S, Imai S, Ando K, Mimura T, Okumura N, Matsusue Y.
  • Organizer: OARSI World Congress on Osteoarthritis 2008
  • Place of Presentation: Rome, Italy
  • Year and Date: 2008-09-19
• [Presentation] Effect of dynamic compressive loading in combination with growth factor on the cartilage-specific gene expression of 3D scaffold-embedded chondrocytes (2007)
  • Author(s): Ando K, Imai S, Isoya E, Kubo M, Okumura N, Mimura T, Matsusue Y.
  • Organizer: OARSI World Congress on Osteoarthritis 2007
  • Place of Presentation: Fort Lauderdale, Florida, USA
  • Year and Date: 2007-12-07
• [Presentation] A novel concentration-gradient collagen scaffold augments due (2007)
  • Author(s): Mimura T, Imai S, Kubo M, Isoya E, Okumura N, Ando K, Matsusue Y.
  • Organizer: OARSI World Congress on Osteoarthritis 2007
  • Place of Presentation: Fort Lauderdale, Florida, USA
  • Year and Date: 2007-12-07
• [Presentation] 高解像度3D・CTを用いた骨粗霧症患者腰椎の骨梁構造解析 (2007)
  • Author(s): 今井 晋二
  • Organizer: 第44回日本リハビリテーション医学会学術集会
  • Place of Presentation: 神戸市
  • Year and Date: 2007-06-08
• [Presentation] Swelling-activated chloride current and its inhibition by arachidonic acid in rabbit articular chondrocytes (2006)
  • Author(s): Isoya E, Matsuura H, Toyoda F, Imai S, Kubo M, Matsusue Y.
  • Organizer: International Cartilage Repair Society 6th Symposium
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2006-01-08
• [Presentation] Collagen scaffold augments large full-thickness articular cartilage repair by active mesenchymal cell recruitment. -detaild cellular events during cartilage repair- (2006)
  • Author(s): Kubo M, Imai S, Isoya E, Ando K, Matsusue Y.
  • Organizer: International Cartilage Repair Society 6th Symposium
  • Place of Presentation: San Diego, CA, USA
  • Year and Date: 2006-01-08
• [Book] 関節軟骨:最新整形外科学大系1.運動器の生物学と生体力学(越智隆弘監修) (2008)
  • Author(s): 今井晋二、松末吉隆
  • Publisher: 東京, 中山書店
• [Book] 関節軟骨 : 最新整形外科学大系1(越智隆弘監修) (2008)
  • Author(s): 今井晋二, 松末吉隆
  • Total Pages: 6
  • Publisher: 中山書店
• [Book] 神経による骨形成の調節について:リウマチ病セミナーXVIII(七川歓次監修) (2007)
  • Author(s): 今井晋二
  • Publisher: 大阪, 長井書店
• [Book] リウマチ病セミナーXVIII(七川歓次監修) (2007)
  • Author(s): 今井 晋二
  • Publisher: 神経による骨形成の調節について