Effect of dynamic compressive loading on three-dimensional scaffold-embedded chondrocytes
| Project/Area Number |
18500400
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Rehabilitation science/Welfare engineering
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| Research Institution | Shiga University of Medical Science |
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Principal Investigator |
IMAI Shinji Shiga University of Medical Science, 医学部, 准教授 (90283556)
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| Co-Investigator(Kenkyū-buntansha) |
MATSUSUE Yoshitaka 滋賀医科大学, 医学部, 教授 (30209548)
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| Project Period (FY) |
2006 – 2008
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| Project Status |
Completed (Fiscal Year 2008)
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| Budget Amount *help |
¥4,020,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥720,000)
Fiscal Year 2008: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2006: ¥900,000 (Direct Cost: ¥900,000)
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| Keywords | 力学的刺激 / 関節軟骨 / 軟骨細胞 / 3次元培養 / コラーゲン |
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| Research Abstract |
軟骨細胞は細胞周囲の環境に応じて、その活性を調節している. 軟骨細胞の脱分化を抑制すべく周期的機械的刺戟を加え、脱分化軟骨細胞の賦活化を試みた. 3次元包埋下圧縮ストレスによってI型コラーゲンの発現が減少し、II型コラーゲンおよびアグリカンの発現が増加し、軟骨細胞の再分化が促されることが明らかになった. BMP-2、b-FGFと機械的刺戟を同時に加えることによる相乗効果を期待したが、逆に効果が弱くなる事が明らかになった.
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Report
(4 results)
Research Products
(29 results)
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[Presentation] Mechanical stress-induced chronological phenotypic changes of chondrocytes in three-dimentional scaffold2008
Author(s)
Ando K, Imai S, Shioji S, Mimura T, Okumura N, Uenaka K, Kasahara T, Nishizawa K, Kubo M, Matsusue Y.
Organizer
OARSI World Congress on Osteoarthritis 2008
Place of Presentation
Rome, Italy
Year and Date
2008-09-19
Related Report
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