• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Electrophysiological Study on gain control over suprachoroidal-transretinal electrical stimulation for retinal prosthesis

Research Project

Project/Area Number 18500402
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Rehabilitation science/Welfare engineering
Research InstitutionOsaka University

Principal Investigator

SAWAI Hajime  Osaka University, Graduate School of Medicine, Associate professor (20202103)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,840,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥240,000)
Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2006: ¥2,800,000 (Direct Cost: ¥2,800,000)
Keywordsartificial vision / retina / electrical stimulation / field potenntials / threshold / superior colliculuss / 人工感覚器 / 再生医学 / 移植・再生医療 / 神経科学 / 生物・生体工学
Research Abstract

1. Introduction
We recently developed an electrical stimulation method for retinal prosthesis named Suprachoroidal-Transretinal Stimulation (STS), and demonstrated that a monophasic pulse of STS focally applied between an anode on the fenestrated sclera and an intravitreal cathode (inward STS) could evoke well-localized field potentials in the superior colliculus (SC) of both normal and retinal dystrophic rats (Kanda et al, 2004). To define effective pulse parameters in STS-based artificial retinas, we here investigated relations of pulse duration (0.1, 0.2, 0.5, 1.0ms), pulse amplitude, and polarities of STS to the collicular responses in rats. We also examined the SC response to biphasic STS comparing with that to the monophasic STS, because biphasic pulses should be used in artificial retina to avoid electrochemical damage to the retina from charge-imbalances.
2. Materials and Methods
16 Long Evans rats and 1 retinal dystrophic rat (Royal College of Surgeons; RCS) were anesthetized and … More immobilized. A single monophasic or biphasic pulse of constant current was delivered as STS to the left eye between an epi-scleral electrode and an intravitreal electrode. Evoked potentials (EP) to STS were recorded from the superficial gray layer of the right SC. Various pulse duration (0.1, 0.2, 0.5, 1.0ms), and pulse amplitude was tested.
3. Results and Discussion
A monophasic inward STS elicited EP that was composed of early and late components with latencies of 5-11 ms and >25ms, respectively. The former consisted of a small positive wave (P1) followed by a large negative wave (N1). The P1-N1 was identified not only in the normal rats but also in the RCS rat. It tended to become larger in amplitude as the pulse duration is shorter at the same quantity of electric charge of STS. To the contrary, the late components were oscillatory, and the amplitude and latency were not affected by the duration of pulse at the same electric charge in the normal rats. Furthermore, the late components were never recognized in the RCS rats. Inverting the polarity of the two stimulation electrodes for a monophasic outward STS, only slow responses were evoked in the normal rats, but not in the RCS rat. The amplitude of the slow responses depended on the electric charge, but not on the duration of the stimulus pulse. On the basis of these results, the P1-N1 component seems to be derived from the direct activation of the retinal ganglion cells by the inward STS. To elicit this component in the STS-based artificial retina, the shorter duration of pulse is more efficient.
A biphasic pulse by a combination of the inward and outward STS elicited the P1-N1, which was comparable in waveform, latency and threshold to that to a monophasic pulse of inward STS. However, the amplitude to the biphasic STS is smaller than that to the inward monophasic STS in most cases. The late oscillatory components were substantially suppressed, especially with short pulses. Such suppression may provide us for high temporal resolution of STS-based artificial retina.
In conclusion, short pulse duration (0.1ms or 0.2ms) of biphasic STS seems to be adequate for an artificial retina. Less

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (31 results)

All 2008 2007 2006

All Journal Article (21 results) (of which Peer Reviewed: 8 results) Presentation (7 results) Book (3 results)

  • [Journal Article] Parameters of optic nerve electrical stimulation affecting neuroprotection of axotomized retinal ganglion cells in adult rats.2008

    • Author(s)
      Okazaki Y. Morimoto T, Sawai H.
    • Journal Title

      Neurosci Res. 61

      Pages: 129-135

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Pikachurin, a dystroglycan ligand, is essential for photoreceptor ribbon synapse formation.2008

    • Author(s)
      Sato S, Omori Y, Katoh K, Kondo M, Kanagawa M, Miyata K, Funabiki K, Koyasu T, Kajimura N, Miyoshi T, Sawai H, Kobayashi K, Tani A, Toda T, Usukura J, Tano Y Fujikado T, Furukawa T.
    • Journal Title

      Nat Neurosci. 11

      Pages: 923-931

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Retinal Prosthesis2008

    • Author(s)
      Sawai H. Miyoshi T, Fujikado H, Tano Y.
    • Journal Title

      The Japanese J. Artifical Organs. 37

      Pages: 163-167

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Parameters of optic nerve electrical stimulation affecting neuroprotection of axotomized retinal ganglion cells in adult rats.2008

    • Author(s)
      Okazaki Y, Morimoto T, Sawai H
    • Journal Title

      Neurosci Res. 61(2)

      Pages: 129-35

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Pikachurin, a dystroglycan ligand, is essential for photoreceptor ribbon synapse formation.2008

    • Author(s)
      Sato S, Omori Y, Katoh K, Kondo M, Kanagawa M, Miyata K, Funabiki K, Koyasu T, Kajimura N, Miyoshi T, Sawai H, Kobayashi K, Tani A, Toda T, Usukura J, Tano Y, Fujikado T, Furukawa T
    • Journal Title

      Nat Neurosci. 11(8)

      Pages: 923-31

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Single-unit responses in lateral geniculate nucleus evoked by a single pulse of suprachoroidal-transretinal stimulation.2008

    • Author(s)
      Sawai H, Miyoshi T, Fujikado H, Tano Y
    • Journal Title

      The Japanese J. Artifical Organs 37

      Pages: 163-167

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Parameters of optic nerve electrical stimulation affecting neuroprotection of axotomized retinal ganglion cells in adult rats2008

    • Author(s)
      Okazaki Y, Morimoto T, and Sawai H
    • Journal Title

      Neurosci Res (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Optical Imaging to evaluate retinal activation by electrical crrents using supra choroidal-transretinal stimuration.2007

    • Author(s)
      Okawa Y, Fujikado T, Miyoshi T, Sawai H, Kusaka S, Mihashi T, Hirohara Y, Tano Y
    • Journal Title

      Invest Ophthalmol Vis Sci. 48

      Pages: 4777-4784

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Effects of adiponectin on the renal sympathetic nerve activity and blood pressure in rats.2007

    • Author(s)
      Tanida M, Shen J, Horii Y, Matsuda M, Kihara S, Funahashi T, Shimomura I, Sawai H, Fukuda Y, Matsuzawa Y, Nagai K.
    • Journal Title

      Exp Biol Med. 232

      Pages: 390-397

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Optical recording of retinal and visual cortical responses evoked by electrical stimulation.2007

    • Author(s)
      Osanai M, sakaehara H, Sawai H, Song W. J, Yagi T.
    • Journal Title

      IEEJ Trans. EIS 127

      Pages: 1595-1602

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Development of retinal implant using a new stimulating method.2007

    • Author(s)
      Sawai H.
    • Journal Title

      Medical Bio 4

      Pages: 36-41

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Optical imaging to evaluate retinal activation by electrical currents using suprachoroidal-transretinal stimulation.2007

    • Author(s)
      Okawa Y, Fujikado T, Miyoshi T, Sawai H, Kusaka S, Mihashi T, Hirohara Y, Tano Y
    • Journal Title

      Invest Ophthalmol Vis Sci. 48(10)

      Pages: 4777-84

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effects of adiponectin on the renal sympathetic nerve activity and blood pressure in rats.2007

    • Author(s)
      Tanida M, Shen J, Horii Y, Matsuda M, Kihara S, Funahashi T, Shimomura I, Sawai H, Fukuda Y, Matsuzawa Y, Nagai K
    • Journal Title

      Exp Biol Med (Maywood). 232(3)

      Pages: 390-7

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Development of retinal implant using a new stimulating method.2007

    • Author(s)
      Sawai H
    • Journal Title

      Medixal Bio 4

      Pages: 36-41

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Humayun Artificial Sight: Basic Research, Biomedical Engineering, and Clinical Advances.2007

    • Author(s)
      Mark S
    • Journal Title

      Springer-Verlag

      Pages: 31-45

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] All about vision and eyemovement.2007

    • Author(s)
      Wakakura M
    • Journal Title

      What is retinal prosthesis.

      Pages: 110-113

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Optical imaging to evaluate retinal activation by electrical currents using suprachoroidal-transretinal stimulation.2007

    • Author(s)
      Okawa Y, Fujikado T, Miyoshi T, Sawai H, Kusaka S, Mihashi T, Hirohara Y, Tano Y.
    • Journal Title

      Invest Ophthalmol Vis Sci. 48

      Pages: 4777-4784

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Optical recording of retinal and visual cortical responses evoked by electrical stimulation2007

    • Author(s)
      Osanai M, Sakaehara H, Sawai H, Song W-J, Yagi T.
    • Journal Title

      IEEJ Trans. EIS 127

      Pages: 1595-1602

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 新しい刺激法を用いた人工網膜の開発2007

    • Author(s)
      澤井元
    • Journal Title

      メディカルバイオ 4

      Pages: 36-41

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Dose-different effects of orexin-A on the renal sympathetic nerveand blood pressure in urethane-anesthetized rats.2006

    • Author(s)
      Tanida, M, Niijima, A, Shen, J, Yamada, S, Sawai, H, Fukuda, Y, Nagai, K
    • Journal Title

      Exp Biol Med 231

      Pages: 1616-1625

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Dose-different effects of orexin-A on the renal sympathetic nerve and blood pressure in urethane-anesthetized rats.2006

    • Author(s)
      Tanida, M, Niijima, A, Shen, J, Yamada, S, Sawai, H, Fukuda, Y, Nagai, K
    • Journal Title

      Exp Biol Med 231

      Pages: 1616-1625

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Pulse parameters affecting rat collicular evoked potentials to Suprachoroidal-Transretinal Electrical Stimulation for retinal prosthesis2007

    • Author(s)
      H. Sawai
    • Organizer
      The Association for Research in Vision and Ophthalmology
    • Place of Presentation
      フォートローダーデール、フロリダ州、米国
    • Year and Date
      2007-05-06
    • Related Report
      2007 Annual Research Report
  • [Presentation] Pulse parameters affecting rat collicular evoked potentials to Suprachoroidal-Transretinal Electroical Stimulation for retinal prosthesis2007

    • Author(s)
      Sawai H.
    • Organizer
      ARVO Annual Meeting
    • Place of Presentation
      フォードローダーデール、フロリダ州、アメリカ合衆国
    • Year and Date
      2007-05-04
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Pulse parameters affecting rat collicular evoked potentials to suprachoroidal-transretinal electrical stimulation for retinal prosthesis.2007

    • Author(s)
      H. Sawai, M. Kuroda, T. Fujikado, Y. Tano
    • Organizer
      ARVO Annual Meeting Abstract P-652, USA
    • Place of Presentation
      Fort Lauderdale, Florida
    • Year and Date
      2007-05-04
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] pulse amplitude, and polarity affecting rat collicular evoked potentials to suprachoroidal-transretinal electrical stimulation for retinal prosthesis.2006

    • Author(s)
      Sawai H.
    • Organizer
      The Fourth Asian Conference on Vision
    • Place of Presentation
      Matsue, Japan
    • Year and Date
      2006-08-01
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Pulse duration, pulse amplitude, and polarity affecting rat collicular evoked potentials to Suprachoroidal suprachoroidal transretinal electrical stimulation for retinal prosthesis.2006

    • Author(s)
      Sawai H, Kuroda M, Miyoshi T, Kanda H, Fujikado T, Tano Y
    • Organizer
      The Fourth, Asian Conference on Vision (ACV2006) abstract P-113
    • Place of Presentation
      Matsue, Japan
    • Year and Date
      2006-08-01
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Single-unit responses in lateral geniculate nucleus evoked by a single pulse of suprachoroidal-transretinal stimulation.2006

    • Author(s)
      Miyoshi T, Kanda H, Fujikado T, Tano Y, Sawai H.
    • Organizer
      The Fourth Asian Conference on Vision
    • Place of Presentation
      Matsue, Japan
    • Year and Date
      2006-07-29
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Single-unit responses in lateral geniculate nucleus evoked by a single pulse of suprachoroidal"transretinal stimulation.2006

    • Author(s)
      Miyoshi T, Kanda H, Fujikado T, Tano Y, Sawai H
    • Organizer
      The Fourth Asian Conference on Vision (ACV2006) abstract P-109
    • Place of Presentation
      Matsue, Japan
    • Year and Date
      2006-07-29
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] Artificial Sight : Basic Research, Biomedical Engineering, and Clinical Advances2007

    • Author(s)
      Mark S. Humayun (Editor)
    • Publisher
      Springer-Verlag
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 視覚と眼球運動のすべて2007

    • Author(s)
      若倉雅登
    • Publisher
      メジカルビュー社、東京
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 視覚と眼球運動のすべて2007

    • Author(s)
      若倉雅登 他(澤井元)
    • Total Pages
      279
    • Publisher
      株式会社メジカルビュー社
    • Related Report
      2006 Annual Research Report

URL: 

Published: 2006-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi