Early rehabilitative approach enhances expression of neurotrophic factor and synaptic plasticity in cortex on the rat cerebral infarction

• Project/Area Number: 18500410
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Rehabilitation science/Welfare engineering
• Research Institution: Kagoshima University
• Principal Investigator: OOWATASHI Akihiko Kagoshima University, Faculty of Medicine, Assistant Professor (30295282)
• Co-Investigator(Kenkyū-buntansha): IKEDA Satoshi Kagoshima University, Medical and Dental Hospital, Senior Assistant Professor (00343369) ; KAWAHIRA Kazumi Kagoshima University, Graduate School of Medical and Dental Sciences, Professor (20117493) ; YOSHIDA Akira Kagoshima University, Graduate School of Medical and Dental Sciences, Assistant Professor (40347109) ; SAKAE Kiyohiro Kagoshima University, Faculty of Medicine, Professor (70041358)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥2,000,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥300,000); Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2006: ¥700,000 (Direct Cost: ¥700,000)
• Keywords: physical therapy / cortical plasticity / GDNF / the model of cerebral infarction / リハビリテーション / 早期運動療法 / 神経栄養因子

Research Abstract

There is limited evidence regarding the effects of rehabilitation on functional recovery on stroke patients. In particular, there are few studies focusing on the relationship of exercise, functional recovery and the expression of glial cell line-derived neurotrophic factor (GDNF).Objectives : We investigated the difference of functional recovery between non-exercise and beam-walking exercise group, and the no expression of GDNF on hemiplegic rat with exercise was examined. Methods : 22 adult Wistar rats (231±12g) were used. These animals were divided into two groups : non-exercise (n=11) and beam-walking exercise (n=11). All rats underwent surgical photochemical infarction at the age of 7 weeks. Rats in the beam-walking exercise group, beam-walking exercises were performed for 20 min everyday for 14 days and non-exercise group were left to follow a natural course. Both groups were evaluated every day with performance scale. 42 adult Wistar rats (232±9.5g) were used. These animals were divided into two groups : non-exercise (n =24),and beam-walking exercise (n=18). At various time points (1 day, 3 days, 5 days, 7 days and 14 days)after photochemical infarction, the brains were removed under deep anesthesia. The expression of GDNF was examined by immunohistochemical analyses. Results : Functional recovery of the beam-walking exercise group was significantly earlier than that of the non-exercise group. On 3 days after infarction, GDNF positive cells of the beam-walking exercise group in the temporal cortex surrounding the infarction were significantly higher than that of the non-exercise group. At the same time window, rats recovered motor function remarkably and the number of GDNF-like immunoreactive cells reached the maximum. Conclusions : The role of GDNF protein after photochemical infarction might be caused the plasticity of the brain rather than the nerve protection.

Research Products

• [Journal Article] Effect of Menthol on Detrusor Smooth-Muscle Contraction and the Micturition Reflex in Rats (2008)
  • Author(s): Nomoto Y, Yoshida A, Ikeda S, Kamikawa Y, Harada K, Ohwatashi A, Kawahira K
  • Journal Title: Urology (in press)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Effect of Menthol on Detrusor Smooth-Muscle Contraction and the Micturition Reflex in Rats (2008)
  • Author(s): Nomoto, Y., Yoshida, A., Ikeda, S., Kamikawa, Y., Harada, K., Ohwatashi, A., Kawahira, K.
  • Journal Title: Urology (in press)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Effect of Menthol on Detrusor Smooth-Muscle Contraction and the Micturition Reflex in Rats. (2008)
  • Author(s): Nomoto Y, Yoshida A, Ikeda S, Kamikawa Y, Harada K, Ohwatashi A, Kawahira K.
  • Journal Title: Urology (in press)
  • Peer Reviewed
• [Journal Article] Functional recovery and expression of GDNF seen in photoch emicallly induced cerebral infarction (2007)
  • Author(s): Keisuke Horinouchi, Satoshi Ikeda, Katsuhiro Harada, Akihiko Ohwatashi, Yurie Kamikawa, Akira Yoshida, Yoshiko Nomoto, Seiji Etoh, Kazumi Kawahira
  • Journal Title: Int J Neurosci. 117 Pages: 315-326
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Functional recovery and expression of GDNF seen in photochemicallly induced cerebral infarction (2007)
  • Author(s): Keisuke, Horinouchi, Satoshi, Ikeda, Katsuhiro, Harada, Akihiko, Ohwatashi, Yurie, Kamikawa, Akira, Yoshida, Yoshiko, Nomoto, Seiji, Etoh, Kazumi, Kawahira
  • Journal Title: Int J Neurosci 117 Pages: 315-326
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Functional recovery and expression of GDNF seen in photochemically induced cerebral infarction (2007)
  • Author(s): Akihiko Ohwatashi
  • Journal Title: Intern.J.Neuroscience 117・3 Pages: 315-326
• [Presentation] Muscle related gene expression on passive repetitive stretching of skeletal muscle (2008)
  • Author(s): Satoshi, Ikeda, Katsuhiro, Harada, Yurie, Kamikawa, Akihiko, Ohwatashi, Keisuke, Horinouchi, Akira, Yoshida, Yoshiko, Nomoto, Kazumi, Kawahira
  • Organizer: Association of academic physiatrist annual meeting
  • Place of Presentation: Anaheim CA
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Effects of edaravone : a free radical scavenger, on hemiplegic model of rat cerebral infarction (2008)
  • Author(s): Katsuhiro, Harada, Satoshi, Ikeda, Akihiko, Ohwatashi, Yurie, Kamikawa, Akira, Yoshida, Kazumi, Kawahira
  • Organizer: Association of academic physiatrist annual meeting
  • Place of Presentation: Anaheim CA
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Effects of menthol on micturition in rats with spinal cord injury (2007)
  • Author(s): Akira, Yoshida, Yoshiko, Nomoto, Satoshi, Ikeda, Yurie, Kamikawa, Katsuhiro, Harada, Akihiko, Ohwatashi, Kazumi, Kawahira
  • Organizer: 4th World Congress of the International Society of Physical and Rehabilitation
  • Place of Presentation: Seoul
  • Year and Date: 2007-06-11
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Effect of treadmill and beam-walking exercise on functional recovery and expression of GDNF after cerebral infarction in rats (2007)
  • Author(s): Akihiko Ohwatashi, Satoshi Ikeda, Keisuke Horinouchi, Katsuhiro Harada, Yurie Kamikawa, Akira Yoshida, Yoshiko Nomoto, Kiyohiro Sakae, Kazumi Kawahira
  • Organizer: 15th International WCPT Congress
  • Place of Presentation: Vancouver Canada
  • Year and Date: 2007-06-05
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Effect of treadmill and beam-walking exercise on functional recovery and expression of GDNF after cerebral infarction in rats. (2007)
  • Author(s): Akihiko Ohwatashi, Satoshi Ikeda, Keisuke Horinouchi, Katsuhiro Harada, Yurie Kamikawa, Akira Yoshida, Yoshiko Nomoto, Kiyohiro Sakae, Kazumi Kawahira
  • Organizer: 15th International WCPT Congress
  • Place of Presentation: Vancouver Canada
  • Year and Date: 2007-06-05
• [Presentation] Photochemical infarctionモデルラットに対して運動が与える影響 (2007)
  • Author(s): 大渡 昭彦, 池田 聡, 原田 雄大, 上川 百合恵, 堀ノ内 啓介, 野元 佳子, 吉田 輝, 川平 和美
  • Organizer: 第42回日本理学療法学術大会
  • Place of Presentation: 新潟
  • Year and Date: 2007-05-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] The 15 minutes passive stretch in several days increases MyoD, myogenin and Myosin heavy chain mRNA expression in the rat medial eastrocunemius muscle (2007)
  • Author(s): Yurie, Kamikawa, Satoshi, Ikeda, Katsuhiro, Harada, Akira, Yoshida, Yoshiko, Nomoto, Akihiko, Ohwatashi, Kazumi, Kawahira
  • Organizer: 4th World Congress of the International Society of Physical and Rehabilitation Medicine Seoul
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Functional recovery on the rat hemiplegic model of cerebral thrombosis (2007)
  • Author(s): Satoshi, Ikeda, Katsuhiro, Harada, Akihiko, Oowatashi, Yurie, Kamikawa, Akira, Yoshida, Keisuke, Horinouchi, Yoshiko, Nomoto, Kazumi, Kawahira
  • Organizer: 4th World Congress of the International Society of Physical and Rehabilitation Medicine Seoul
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Effect of treadmill and beam-walking exercise on functional recovery and expression of GDNF after cerebral infarction in rats (2007)
  • Author(s): Akihiko, Ohwatashi, Satoshi, Ikeda, Keisuke, Horinouchi, Katsuhiro, Harada, Yurie, Kamikawa, Akira, Yoshida, Yoshiko, Nomoto, Kiyohiro, Sakae, Kazumi, Kawahira
  • Organizer: 15th International WCPT Congress
  • Place of Presentation: Canada
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Expression of muscular growth factor on passive stretching in the rat skeletal muscle (2006)
  • Author(s): Ikeda, S., Kamikawa, Y., Harada, K., Oowatashi, A., Horinouchi, K., Yoshida, A., Nomoto, Y., Kawahira, K
  • Organizer: ACRM-ASNR Joint Educational Conference
  • Place of Presentation: Boston
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Functional recovery from second contralateral infarction on the rat hemiplegic model (2006)
  • Author(s): Katsuhiro, Harada, Satoshi, Ikeda, Keisuke, Horinouchi, Yurie, Kamikawa, Akira, Yoshida, Yoshiko, Nomoto, Akihiko, Ohwatashi, Kazumi, Kawahira
  • Organizer: ACRM-ASNR Joint Educational Conference
  • Place of Presentation: Boston
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Intention exercise is more effective for functional recovery than simple exercise on the rat experimental model of hemiplegia (2006)
  • Author(s): Satoshi, Ikeda. Akihiko, Oowatashi, Keisuke, Horinouchi, Yurie, Kamikawa, Akira, Yoshida, Yoshiko, Nomoto, Kazumi, Kawahira, Nobuyuki, Tanaka
  • Organizer: 4th World Congress for NeuroRehabilitation
  • Place of Presentation: Hong Kong
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] Ch 8, Passive repetitive stretches activate the skeletal muscle and induce the expression of mRNA of transcription factors.Frank Columbus Ed.New Messenger RNA Research Communications (2007)
  • Author(s): Satoshi Ikeda, Yurie Kamikawa, Katsuhiro Harada, Akihiko Oowatashi, Akira Yoshida, Keisuke Horinouchi, Kazumi Kawahira
  • Publisher: Nova Science Publishers,Inc.NY.
  • Description: 「研究成果報告書概要(和文)」より
• [Book] Ch 8, Passive repetitive stretches activate the skeletal muscle and induce the expression of Mrna of transcription factors. Frank Columbus Ed. New Messenger RNA Research Communications (2007)
  • Author(s): Satoshi, Ikeda, Yurie, Kamikawa, Katsuhiro, Harada, Akihiko, Oowatashi, Akira, Yoshida, Keisuke, Horinouchi, Kazumi, Kawahira
  • Publisher: Nova Science Publishers, Inc. NY
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] Ch 8, Passive repetitive stretches activate the skeletal muscle and induce the expression of mRNA of transcription factors. Frank Columbus Ed. New Messenger RNA Research Communications. (2007)
  • Author(s): Satoshi Ikeda, Yurie Kamikawa, Katsuhiro Harada, Akihiko Oowatashi, Akira Yoshida, Keisuke Horinouchi, Kazumi Kawahira
  • Publisher: Nova Science Publishers, Inc. NY.
• [Book] Robert Taylor (authors), Yasuaki Kawasaki, Akihiko Ohwatashi, Makoto Hujiki (translation supervisor). Saunders Veterinary Clinics Small Animal Practice. Vol.1-6 (2006)
  • Author(s): David, Levine, Darryl, L., Millis, Denis, J., Marcellin-Little
  • Publisher: Inter Zoo Co.,Ltd Tokyo
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] サンダースベテリナリークリニクスシリーズVol.1 No.6 リハビリテーションと理学療法 (2006)
  • Author(s): 大渡昭彦
  • Total Pages: 224
  • Publisher: 株式会社インターズー、東京