| Project/Area Number |
18500506
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Sports science
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| Research Institution | Akita University |
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Principal Investigator |
NANJO Hiroshi Akita University, School of medicine, Lecturer (70250892)
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| Co-Investigator(Kenkyū-buntansha) |
MASUDA Hirotake Akita University, School of medicine, professor (60103462)
KAWAMURA Koichi Akita University, School of medicine, associate professor (00091801)
TAKAHASHI Masato Akita University, School of medicine, associate professor (10315806)
KOBAYASHI Mikio Akita University, School of medicine, technician (20375306)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,060,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | endothelial cell / bone marrow origin / capillary / heart / vascular remodeling / aorta / striated muscle / training / 骨髄由来 / 樹状細胞 / 心臓弁 |
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| Research Abstract |
In vivo localization and distribution of endothelial cells of bone marrow origin has not well known in systemic organs. So we investigated their localization and distribution in special reference to physical training using bone marrow transplantation (BMT) chimeric mice using male C57BL/6J mice as recipients and male GFP mice (C57BL/6J-Tg: CAG-EGFP transgenic mouse) as donors. The results were as follows. A lot of GFP-positive CD34-positive, MIB-5 positive, and Brdu-positive endothelial cells of donor bone marrow origin were present at the cardiac capillaries, cardiac valves, ascending aorta orifice, renal capillaries in the BMT chimeric recipient mice. Endothelial cells of bone marrow origin were present at the blood-brain barrier, hepatic and splenic sinusoids, and any other general organs. Endothelial cells of bone marrow origin were few at the striated muscle capillaries. But, by physical training of two to four months periods, GFP-positive endothelial cells of bone marrow origin could be more mobilized at the lower limb striated muscle capillaries than control.
Endothelial progenitor cell localization and differentiation may be closely associated with vascular remodeling and hemodynamic conditions. Furthermore, pericytic and dendritic cells of bone marrow origin were present at the cardiac capillaries and aorta. Modulating distribution of endothelial progenitor cells may represent a novel therapeutic strategy in vascular remodeling and cardiovascular disease.
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