| Project/Area Number |
18500516
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Sports science
|
|---|
| Research Institution | Kamakura Women's University |
|---|
Principal Investigator |
YOSHINO Yoko Kamakura Women's University, Faculty of Family and Consumer Sciences, Lecturer (70298248)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
YAMANE Akira Tsurumi University, School of Dental Medicine, Lecturer (20166763)
SUZUKI Masashige Waseda University, School of Sport Sciences, professor (90015842)
NAKAGAWA Yoichi Tsurumi University, School of Dental Medicine, Lecturer (90148057)
|
|---|
| Project Period (FY) |
2006 – 2007
|
| Project Status |
Completed (Fiscal Year 2007)
|
| Budget Amount *help |
¥1,610,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥700,000 (Direct Cost: ¥700,000)
|
|---|
| Keywords | Salivary secretion / Exercise / Food restriction / Salivary protein / Kallikrein activity / alpha-adrenergic receptor / 8-OHdG / 運動 / 酸化ストレス / アドレナリン受容体 / 唾液腺機能 / 唾液タンパク濃度 / α_2-アドレナリン受容体 |
|---|
| Research Abstract |
Objective : The objective of the present study was to examine the effect of exercise training on salivary gland through parasympathetic nervous system.
Materials and methods: Male ICR mice, weighing 39〜50g, at 32 weeks of age were divided into three groups; an exercise with food restriction (EXP), an exercise without food restriction (EXA), and a control (CTL). EXP group uses voluntary running wheel for exercise, and fed the same amount of diet as the CTL group (pair-feeding). The pilocarpine stimulated whole saliva was collected from the oral cavity by micro-pipet over 15 min 4, 8, and 12 weeks after beginning of the exercise with pair-feeding. The salivary flow rate, protein concentration, and amylase and kallikrein activities were determined. Kallikrein and amylase release are evoked by alpha- and beta- adrenergic receptor stimulation, respectively.
Results: The body weight of EXP mice was approximately 20% less than that of CTL mice between 4 and 12 weeks after the beginning of the experiment (p<0.05). Salivary 8-OHdG was significantly increased in the EXP group. The total protein secretion and kallikrein activity decreased 20〜30% in the EXP mice between 4 and 12 weeks after the starting of experiments when compared with the CTL mice(p<0.05〜0.01).
Conclusion : The results showed that the combination of exercise and diet control decreased kallikrein activity from the mice saliva, suggesting that signal transduction in the ∂-sympathetic nervous system was down regulated or the synthesis of kallikrein decreased in the salivary glands of exercise mice. The down-regulation was thought to be due to increse of 8-OHdG. This study suggested that the sufficient food intake was necessary for maintaining the oral function during physical exercise.
|
