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STUDY ON ANTISEPTIC EFFECT OF SILVER AND COPPER IONS AGAINST LEGIONELLA IN HOT SPRING WATERS

Research Project

Project/Area Number 18510035
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Environmental impact assessment/Environmental policy
Research InstitutionToho University

Principal Investigator

KATO Naoyuki  Toho University, Faculty of Medicine, Associate Professor (50104154)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,120,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2006: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsLEGIONELLA / HOT SPRINNG / SILVER ION / COPPER ION / AMOEBA / TROPHOZOITE / CYST / ANTISEPTIC EFFECT / 殺菌作用 / 循環装置 / 温泉環境学
Research Abstract

1. We studied the influence of chemical composition of hot springs to the antiseptic effect of silver ion.
(1) The antiseptic effect of silver ion delayed according to-increases of chloriide and sulfuric acid ions in hot spring. (2) Some hot spring did not show any antiseptic effect despite enough silver ion concentration. The cause will be attributed to that silver ion formed complex with some kind of hot spring components.
2. Antiseptic effect of silver ion against legionella cells in amoeba or amoeba alone was studied.
(1) While legionella cells in amoeba were not killed at the concentration of 0.05 ppm of silver ion, silver ion of 2.5ppm showed an antiseptic effect. (2) Legionella cells in cyst encysted amoeba were not killed even at the concentration of 2.5ppm but did at the concentration of 5ppm. (3) Amoeba cells themselves were not killed by the concentration of-5ppm of silver ion.
3. The concentration of copper ion in an aqueous solution decreased with a rise in pH.
In this study … More , particles coated with copper were added into each aqueous solution with different pH at a final concentration of 0.1%and 0.5%, respectively. Eluted copper concentration was different in 0.1% and 0.5%, however there was no difference among different pH in dissolution rate of copper along with the time course. 4. Antiseptic effect of copper ion against legionella cells in amoeba or amoeba alone was studied.
(1) The antiseptic duct of copper ion against legionella cells m trophozoite cells was not shown at the concentration of 1ppm, while legionella cells of 76% at 5ppm and 95% at 10ppm were killed, respectively. (2) The legionella cell in cyst form were not killed at the concentration of 1ppm of copper ion as well as in trophozoite cell. Eighty fie % and 94% of legionella cells were killed at the concentration of 5ppm and 10ppm, respectively. (3) Seventy four % and 47% of amoeba cells survived in the copper ion concentration of 5ppm and 10ppm, respectively.
5. In the experiment of antiseptic effect against legionella in copper ion solution with pH7.2, Legionella cells were completely killed at 6h after the addition of 0.5 ppm copper ion. On the other hand, each 0.1% copper solution adjusted. to pH6.0, 7.4 and 8.5 showed all antiseptic effect, and distilled water, tap water and marine water adjusted to copper concentration of 0.1% had also the same effect. The antiseptic effect of hot spring waters against legionella was diversed among each hot spring due to the differences of pH and chemical composition of hot springs. Less

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (22 results)

All 2008 2007 2006

All Journal Article (3 results) (of which Peer Reviewed: 2 results) Presentation (19 results)

  • [Journal Article] 微量元素と温泉2007

    • Author(s)
      加藤 尚之
    • Journal Title

      Biomed Res Trace Elements 18

      Pages: 301-310

    • NAID

      10021097424

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Trace Elements in Hot Spring Water2007

    • Author(s)
      N., Kato
    • Journal Title

      Biomed Res Trace Elements 18

      Pages: 301-310

    • NAID

      10021097424

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 微量元素と温泉2007

    • Author(s)
      加藤尚之
    • Journal Title

      Biomed Res Trace Elements 18

      Pages: 301-310

    • NAID

      10021097424

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] Acanthamoeba castellaniiとLegionella pneumophilaの捕食寄生相互関係に及ぼす温度の影響2008

    • Author(s)
      大野 章
    • Organizer
      第81回日本細菌学会総会
    • Place of Presentation
      京都
    • Year and Date
      2008-03-25
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Temperature-dependent parasitic relationship between Legionella pneumophila and a Free-living amoeba (Acanthamoeba castellanii2008

    • Author(s)
      A., Ohno, N., Kato, R., Sakamoto, S., Kimura, K., Yamaguchi
    • Organizer
      The 81th Annual Scientific Meeting of the Japan Society for Bacteriology
    • Place of Presentation
      Kyoto
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 温泉での銅イオンによるレジオネラ殺菌2007

    • Author(s)
      加藤 尚之
    • Organizer
      第60回日本温泉科学会大会
    • Place of Presentation
      鹿児島
    • Year and Date
      2007-09-20
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] レジオネラ属菌が温泉において高濃度で分離培養される要因-各種水温におけるアメーバ内増殖能への影響-2007

    • Author(s)
      大野 章
    • Organizer
      第60回日本温泉科学会大会
    • Place of Presentation
      鹿児島
    • Year and Date
      2007-09-20
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 温泉での銅イオンによるレジオネラ殺菌2007

    • Author(s)
      加藤尚之
    • Organizer
      日本温泉科学会大会
    • Place of Presentation
      かごしま県民交流センター
    • Year and Date
      2007-09-20
    • Related Report
      2007 Annual Research Report
  • [Presentation] Legionella pneumophilaおよびAmoebaに対する銅殺菌効果の検討2007

    • Author(s)
      加藤 尚之
    • Organizer
      第34回日本防菌防黴学会年次大会
    • Place of Presentation
      大阪
    • Year and Date
      2007-08-30
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 温泉と微量元素2007

    • Author(s)
      加藤 尚之
    • Organizer
      第18回日本微量元素学会(シンポジウム)
    • Place of Presentation
      福井
    • Year and Date
      2007-07-05
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 河川に生息するレジオネラの培養不能の原因解明,各種水温におけるアメーバ内増殖能への影響2007

    • Author(s)
      大野 章
    • Organizer
      第80回日本細菌学会総会
    • Place of Presentation
      大阪
    • Year and Date
      2007-03-26
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Cause of non-cultivability of legionella inhabiting in river and effect of diverse temperatures for proliferation ability of legionella in amoeba2007

    • Author(s)
      A., Ohno, N., Kato, K., Yamaguchi
    • Organizer
      The 80th Annual Scientific Meeting of the Japan Society for Bacteriology
    • Place of Presentation
      Osaka
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Hot spring water and Trace Element2007

    • Author(s)
      N., Kato
    • Organizer
      The 18th Annual Scientific Meeting of the Japan Society for Biomedical Research on Trace Elements (Symposium Lecture)
    • Place of Presentation
      Fukui
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Study on antiseptic effect of copper ion against Legionella pneumophila and amoeba2007

    • Author(s)
      N., Kato, K., Yamada, A., Ohno
    • Organizer
      The 34th Annual Scientific Meeting of the Japan Society for Antibacterial and antifungal agents
    • Place of Presentation
      Osaka
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Legionella sterilization of copper ion in hot spring water2007

    • Author(s)
      N., Kato, K., Yamada, A., Ohno, S., Okouchi
    • Organizer
      The 60th Annual Scientific Meeting of the Balneological Society of Japan
    • Place of Presentation
      Kagoshima
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Factor that Legionella pneumoniae is frequently isolated from hot spring water -Effect of diverse temperatures for proliferation ability of legionella in amoeba2007

    • Author(s)
      A., Ohno, N., Kato, K., Yamaguchi
    • Organizer
      The 60th Annual Scientific Meeting of the Balneological Society of Japan
    • Place of Presentation
      Kagoshima
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 温泉とレジオネラ属菌2006

    • Author(s)
      加藤 尚之
    • Organizer
      宮城大学(招待講演)
    • Place of Presentation
      仙台
    • Year and Date
      2006-10-30
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Legionella pneumophilaに対する銅殺菌効果の検討2006

    • Author(s)
      加藤 尚之
    • Organizer
      第59回日本温泉科学会大会
    • Place of Presentation
      秋田
    • Year and Date
      2006-09-06
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Legionella pneumophilaおよびAmoebaに対する銀殺菌効果の検討2006

    • Author(s)
      加藤 尚之
    • Organizer
      第33回日本防菌防黴学会年次大会
    • Place of Presentation
      東京
    • Year and Date
      2006-05-31
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Study on antiseptic effect of silver ion against Legionella pneumophila and amoeba2006

    • Author(s)
      N., Kato, K., Yamada, A., Ohno
    • Organizer
      The 33th Annual Scientific Meeting of the Japan Society for Antibacterial and Antifungal Agents
    • Place of Presentation
      Tokyo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Study on antiseptic effect of copper ion against Legionella pneumophila2006

    • Author(s)
      N., Kato, A., Ohno, K., Yamada, S., Okouchi
    • Organizer
      The 59th Annual Scientific Meeting of the Balneological Society of Japan
    • Place of Presentation
      Akita
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Hot spring water and Legionella spp2006

    • Author(s)
      N., Kato
    • Organizer
      Miyagi University (Invitation Lecture)
    • Place of Presentation
      Sendai
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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