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Establishment of the evaluation system for endocrine-disrupting chemimals using stickleback

Research Project

Project/Area Number 18510060
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Risk sciences of radiation/Chemicals
Research InstitutionNagasaki University

Principal Investigator

NAGAE Masaki  Nagasaki University, Faculty of Environmental Studies, Associate Professor (00315227)

Co-Investigator(Kenkyū-buntansha) HARA Akihiko  Hokkaido University, Graduate school of Fisheries Science, Professor (40091483)
SOYANO Kiyoshi  Nagasaki University, Institute for Easr China Sea Research, Associate Professor (80260735)
URA Kazushiro  Hokkaido University, Graduate school of Fisheries Science, Assistant Professor (90360940)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,140,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥540,000)
Fiscal Year 2007: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
KeywordsBiomarker / Stickleback / Endocrine-disrupting chemical
Research Abstract

Male stickleback produce a glue protein named "spiggin" that is used as a cementing substance for the building of its nest. The synthesis of spiggin is strongly controlled by androgen. Therefore, spiggin is a useful biomarker to evaluate androgenic activity of environmental chemicals, in addition to vitellogenin, precursor of yolk protein produced from the liver by the estrogenic stimulation. In this study, we established the highly sensitive quantification system for both spiggin and vitellogenin to evaluate sex-hormonal effect of chemicals.
Highly sensitive quantification system for spiggin mRNA was established using real-time RT-PCR technique. This system enables precise and high sensitive quantification. Vitellogenin, biomarker for estrogenic stimulation, was also purified by several chromatographic techniques. Specific antibody against vitellogenin was also raised. Furthermore, vitellogenin quantification system was established using the method of chemiluminescent-linked immunosorb … More ent assay. This system also enables high sensitive quantification same with spiggin assay system. In addition to these preparations for quantification systems for the biomarkers, the detail condition for in vivo exposure test using stickleback was determined. Using MT as a control androgen, we performed exposure test. As a result, only 3 days exposure at a MT concentration 0.1 and 1 μg/L was efficient for spiggin mRNA expression in kidney. Next, MT exposure was performed under three different water temperature (5, 10 and 15℃) to know the effect of water temperature on spiggin mRNA expression. Exposure at 15℃ induced maximal MT effect on spiggin mRNA expression, the others, particular in exposure at 5℃, markedly diminished MT effect. This result suggests that at least water temperature from 10℃ and up is necessary to perform high sensitive detection of androgenic activity using spiggin as a biomarker.
The present established systems will be powerful tools for the evaluation of the endocrine disrupting potency of chemicals. Less

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (10 results)

All 2007 2006

All Journal Article (6 results) (of which Peer Reviewed: 4 results) Presentation (4 results)

  • [Journal Article] Detection and assessment of androgenic potency of endocrine-disrupting chemicals using three-spined stickleback,Gasterosteus aculeatus2007

    • Author(s)
      Masaki Nagae
    • Journal Title

      Environmental Sciences 14

      Pages: 255-261

    • NAID

      120006982377

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Three-spined stickleback:anine emerging model in environmental endocrine disruption2007

    • Author(s)
      Ioanna Katsiadaki
    • Journal Title

      Envkonmental Sciences 14

      Pages: 263-283

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Detection and assessment of androgenic potency of endocrine-disrupting chemicals using three-spined stickleback, Gasterosteus aculeatus2007

    • Author(s)
      Masski, Nagae, et. al.
    • Journal Title

      Environmental Sciences 14

      Pages: 255-261

    • NAID

      120006982377

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Three-spined stickleback : an emerging model in environmental endocrine disruption2007

    • Author(s)
      Ioanna, Katsiadaki, et. al.
    • Journal Title

      Environmental Sciences 14

      Pages: 263-283

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Detection and assessment of androgenic potency of endocrine-disrupting chemicas using three-spined stickleback, Gasterosteus aculeatus2007

    • Author(s)
      Masaki Nagae
    • Journal Title

      Environmental Sciences 14

      Pages: 255-261

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Three-spined stickleback: an emerging model in environmental endocrine disruption2007

    • Author(s)
      Ioanna Katsiadaki
    • Journal Title

      Environmental Sciences 14

      Pages: 263-283

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Presentation] イトヨゲノムデータベースに基づくアンドロゲン特異的タンパク(スピギン)mRNAの高感度検出系構築2007

    • Author(s)
      坂井 正頌
    • Organizer
      第10環境ホルモン学会研究発表会
    • Place of Presentation
      大宮ソニックシティー
    • Year and Date
      2007-12-11
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Highly sensitive detection of spiggin mRNA for the evaluation of androgenic potency of chemicals2007

    • Author(s)
      Masanobu, Sakai, et. al.
    • Organizer
      The 9th Annumal Meeting of Japan Society of Endocrine Disrupters Research
    • Place of Presentation
      Omiya Sonic-City(Saitama)
    • Year and Date
      2007-12-11
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] イトヨ雄特異タンパク(スピギン)を用いた環境アンドロゲン作用の高感度検出2006

    • Author(s)
      坂井 正頌
    • Organizer
      第9環境ホルモン学会研究発表会
    • Place of Presentation
      星薬科大学
    • Year and Date
      2006-11-11
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Establishment of highly sensitive quantification sysytem of spiggin mRNA based on stickleback genome database information2006

    • Author(s)
      Masanobu, Sakai, et. al.
    • Organizer
      The 10th Annumal Meeting of Japan Society of Endocrine Disrupters Research
    • Place of Presentation
      HOSHI University(Tokyo)
    • Year and Date
      2006-11-11
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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