| Project/Area Number |
18510062
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Risk sciences of radiation/Chemicals
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
HANDA Osamu Kyoto Prefectural University of Medicine, Department of Biomedical Safety Science, Assistant Professor (90381970)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Toshikazu Kyoto Prefectural University of Medicine, Department of Inflammation and Immunology, Professor (90158410)
YOSHIDA Norimasa Kyoto Prefectural University of Medicine, Department of Molecular Gastroenterology and Hepatology, Assistant Professor (30166962)
NAITO Yuji Kyoto Prefectural University of Medicine, Department of Medical Proteomics, Associate Professor (00305575)
KOKURA Satoshi Kyoto Prefectural University of Medicine, Department of Biomedical Safety Science, Associate Professor (80347442)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,650,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥450,000)
Fiscal Year 2007: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | cosmetic additives / toxicology / ultra violet / genomic analysis / keratinocvte |
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| Research Abstract |
For many years, methylparaben (MP) has been used as a preservative in cosmetics. In this study, we investigated the effects of ultraviolet-B(UVB)exposure on MP-treated human skin keratinocytes. HaCaT keratinocyte was cultured in MP-containing medium for 24 h, exposed to UVB (15 or 30 mJ/cm2) and further cultured for another 24 h. Subsequent cellular viability was quantified by MTT-based assay and cell death was qualified by fluorescent microscopy and flow cytometry. Oxidative stress, nitric oxide (NO) production and cellular lipid peroxidation were measured using fluorescent probes. In addition, activation of nuclear factor kappa B and activator protein-1 was assessed by electro-mobility gel-shift assay. Practical concentrations of MP (0.003%) had a little or no effect on cellular viability, oxidative stress, NO production, lipid peroxidation and activation of nuclear transcription factors in HaCaT keratinocytes. Low-dose UVB also had little or no effect on these parameters in HaCaT keratinocytes. However, UVB exposure significantly increased cell death, oxidative stress, NO production, lipid peroxidation and activation of transcription factors in MP-treated HaCaT keratinocytes. These results indicate that MP, which has been considered a safe preservative in cosmetics, may have harmful effects on human skin when exposed to sunlight.
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