| Project/Area Number |
18510076
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Environmental technology/Environmental materials
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| Research Institution | Kyushu Institute of Technology |
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Principal Investigator |
OGAWA Hiroaki Kyushu Institute of Technology, Graduate School of Life Science and Systems Engineering, Professor (50108685)
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| Co-Investigator(Kenkyū-buntansha) |
SHIRAI Yoshihito Kyushu Institute of Technology, Graduate School of Life Science and Systems Engineering, Professor (50175395)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,990,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | Sewage excess sludge / Sludge-lysing bacteria / Sludee-lysing canacity / Lactic acid nroduction / Protease / Environmentally-friendly technology / 下水剰余汚泥 / 下水汚泥分解細菌株 / プロテアーゼ放出 |
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| Research Abstract |
For the purpose of reducing industrial waste, activated sludge, Brevibacillus sp. KH3 and Bacillus sp. KH4 were isolated from sewage excess sludge ; these strains can decrease up to about 30% of the excess sludge at 50℃ and 37℃, respectively. Strains KH3 and KH4 had high protease activities when they were incubated with sewage excess. A casein zymography for evaluating protease activity revealed that KH3 released two proteases (32 and 40 kDa) and KH4 produced three protease activities (30, 44 and 250 kDa). Nutrient-rich conditions (excess sludge plus LB medium) strongly inhibited the degradation of excess sludge by KH3. Since 40 kDa protease was detected incubation under nutrient-poor environment(excess sludge without LB)but not during nutrient-rich condition, the 40 kDa protease seems to be involved in degradation of sludge by KH3.
Next, we were isolated and identified Lactobacillus acidophilus, L. delbrueckii subsp. delhrueckii and L. laclis subsp. laclis from the excess sludge containing 50 mM of sucrose at 50℃. Also, L. plantarum, L. delb. delb. and L. pentasus were found from the sludge medium at 30℃. In addition, lactic acid production from dissolved the sludge by KH3 and KH4 with 50 mM of sucrose by L. acidophilus was examined. Lactic acid yield was found to show an increasing effect; the dissolved sludge medium gave 9 and 6 fold more lactic acid production than unsolved sludge medium in KH3 and KH4, respectively.
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