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Development of a micro fluorescence detector for m-TAS based on surface plasmon field enhancement

Research Project

Project/Area Number 18510110
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Microdevices/Nanodevices
Research InstitutionKyoto Prefectural University

Principal Investigator

ISHIDA Akito  Kyoto Prefectural University, 人間環境学部, 助教授 (20184525)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥2,410,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
Keywordsplasmon enhanced excitation / micro total analysis system / silver-coated nanocone array / fluorescence immunoassay / liauid phase silver coating / nanoimprinting / micro fluidics / PDMS
Research Abstract

The aim of this study is development of a high-performance fluorescence detection method based on surface plasmon enhanced excitation for integration into a micro total analysis system (μ-TAS). We employed a PMMA nanocone array (diameter, 250nm ; height, 750 nm ; pitch, 250 nm ; macroscopic dimension, 5×5mm^2) prepared by nanoimprinting as a substrate. The surface was treated with tannic acid and silver nitrate successively. After immobilization of Texas-Red on the surface, fluorescence enhancement property of the nanocone array was estimated by comparison between the fluorescence intensity of the same areas in the presence and absence of nanocone array upon far-field illumination using a fluorescence microscope. The silver-coated nanocone array showed the fluorescence intensity approximately 50 times larger than that of the flat surface. This result suggests that far-field illumination of the silver-coated nanocone array induces local plasmons and the strongly enhanced electromagnetic field effectively excites the fluorophores immobilized on the surface. Then we have demonstrated a practical application of this excitation method. Anti-IgG was immobilized on a silver coated nanocone arrays and the two sections of the surface were covered with PDMS microfluid channels, respectively. Fluorescence labeled IgG solution and BSA solution were independently introduced into the two channels and then, after careful washing, fluorescence microscopic imaging was carried out. The clear difference in the fluorescence intensity between the two channels demonstrates that the silver-coated nanocone is useful for fluorescence bioassays as a plasmon-enhanced substrate.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (27 results)

All 2008 2007 2006

All Journal Article (11 results) (of which Peer Reviewed: 1 results) Presentation (10 results) Book (6 results)

  • [Journal Article] Lanthanide Complex Strategy for Detection and Separation of Histidine-tagged Proteins2007

    • Author(s)
      Hiroshi Tsukube, Keiko Yano, Akito Ishida, Satoshi Shinoda
    • Journal Title

      Chem.Lett. 36

      Pages: 544-545

    • NAID

      10018881250

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Lanthanide Complex Strategy for Detection and Separation of Histidine-tagged Proteins2007

    • Author(s)
      Hiroshi Tsukube, Keiko Yano, Akito Ishida, Satoshi Shi-noda
    • Journal Title

      Chem.Lett. 36

      Pages: 544-545

    • NAID

      10018881250

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 金属ナノ構造体を用いるプラズモン増強励起蛍光分析2007

    • Author(s)
      石田 昭人
    • Journal Title

      日本写真学会誌 70

      Pages: 8-13

    • NAID

      10018577197

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Lanthanide Complex Strategy for Detection and Separation of Histidine-tagged Proteins2007

    • Author(s)
      Hiroshi Tsukube, Keiko Yano, Akito Ishida, Satoshi Shinoda
    • Journal Title

      Chem. Lett 36

      Pages: 544-555

    • NAID

      10018881250

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Disruption of the psbA gene by the copy correction mechanism reveals that the expression of plastid-encoded genes is regulated by photosynthesis activity2007

    • Author(s)
      M.Khan, W.Hameed, M.Nozoe, T.Shiina
    • Journal Title

      J Plant Res. 120

      Pages: 421-430

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 金属ナノ構造体を用いるプラズモン増強励起蛍光分析2007

    • Author(s)
      石田昭人
    • Journal Title

      日本写真学会誌 70

      Pages: 8-13

    • NAID

      10018577197

    • Related Report
      2006 Annual Research Report
  • [Journal Article] プラズモン・バイオエネルギー変換素子2006

    • Author(s)
      石田昭人, 椎茗隆, 竹田津悠
    • Journal Title

      光アライアンス 12

      Pages: 24-28

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Plasmon-enhanced Biological Light-energy Conversion Devices(in Japanese)2006

    • Author(s)
      Akito Ishida, Takashi, Shiina, Haruka Takedatsu
    • Journal Title

      Photo Alliance 12

      Pages: 24-28

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Surface Plasmon Enhanced Excitation of Photofunctional Molecules in Nanospace towards Molecular Plasmonics2006

    • Author(s)
      Ayako Fujii, Akito Ishida
    • Journal Title

      Handai Nanophotonics. Nanoplasmonics from Fundamentals to Applications(ed by Kawata, S.; Masuhara, H.)(Elsevier, Amsterdam) 2

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Plasmon enhanced fluorescence analyses in nanospace for bio-sensing(in Japanese)2006

    • Author(s)
      Akito Ishida, Ayako Fujii
    • Journal Title

      Design and Applications of Plasmonic Nano Materials(ed by S.Yamada)(CMC Press., Tokyo)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] プラズモン・バイオエネルギー変換素子2006

    • Author(s)
      石田昭人, 椎名隆, 竹田津悠
    • Journal Title

      光アライアンス 18

      Pages: 24-28

    • Related Report
      2006 Annual Research Report
  • [Presentation] プラズモン蛍光増強媒体としての金属コートナノインプリントレプリカと金コロイド吸着基板2008

    • Author(s)
      石田昭人
    • Organizer
      日本化学会第88春季年会
    • Place of Presentation
      立教大学池袋キャンパス
    • Year and Date
      2008-03-29
    • Related Report
      2007 Annual Research Report
  • [Presentation] プラズモン増強媒体としての金属コートナノインプリントレプリカと金コロイド吸着基板2008

    • Author(s)
      石田昭人
    • Organizer
      日本化学会第88春季年会
    • Place of Presentation
      東京
    • Year and Date
      2008-03-26
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] プラズモン増強電場のバイオフォトニクスへの応用2007

    • Author(s)
      石田昭人
    • Organizer
      応用物理学会応用電子物性分科会研究例会
    • Place of Presentation
      東京
    • Year and Date
      2007-10-25
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] プラズモン増強励起を用いるミュータス用微小蛍光検出器の開発研究2007

    • Author(s)
      石田昭人
    • Organizer
      2007年レーザー学会東京支部セミナー
    • Place of Presentation
      慶応大学理工学部
    • Year and Date
      2007-07-06
    • Related Report
      2007 Annual Research Report
  • [Presentation] ナノ構造化金属表面におけるプラズモン増強蛍光分析2007

    • Author(s)
      石田昭人
    • Organizer
      レーザー学会第18回若手技術者と学生のためのレーザー応用セミナー
    • Place of Presentation
      東京
    • Year and Date
      2007-06-07
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 希土類添加透明ナノ結晶化ガラスの表面プラズモン励起アップコンバージョン発光2007

    • Author(s)
      上田純平、石田昭人、田部勢津久
    • Organizer
      第24回希土類討論会
    • Place of Presentation
      九州大学医学部百年講堂
    • Year and Date
      2007-05-16
    • Related Report
      2007 Annual Research Report
  • [Presentation] 局在プラズモン増強蛍光によるバイオセンシング2007

    • Author(s)
      石田昭人
    • Organizer
      日本化学会第87春季年会
    • Place of Presentation
      大阪
    • Year and Date
      2007-03-25
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 微小構造体によるプラズモン電場増強と蛍光分析への応用2006

    • Author(s)
      石田昭人
    • Organizer
      日本分光学会関西支部講演会「最近の分光学の進歩に関する講演会-表面プラズモン、近接場を利用した分光」
    • Place of Presentation
      大阪
    • Year and Date
      2006-11-16
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 微小構造体による蛍光増強とその応用2006

    • Author(s)
      石田昭人
    • Organizer
      分子構造総合討論会
    • Place of Presentation
      静岡
    • Year and Date
      2006-09-20
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 増強プラズモンとナノバイオ蛍光分析2006

    • Author(s)
      石田昭人, 藤井亜矢子
    • Organizer
      光化学討論会(招待講演)
    • Place of Presentation
      仙台
    • Year and Date
      2006-09-10
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] プラズモン分光,分子認識と超分子,早下隆士,築部浩編,6章 先端分子認識化学のための解析ツール,97-1042007

    • Author(s)
      石田昭人
    • Total Pages
      7
    • Publisher
      三共出版
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] 分子認識と超分子 第6章2007

    • Author(s)
      石田昭人(共著)
    • Total Pages
      237
    • Publisher
      三共出版
    • Related Report
      2007 Annual Research Report
  • [Book] Surface Plasmon Enhanced Excitation of Photofunctional Molecules in Nanospace towards Molecular Plasmonics Handai Nanophotonics ed by Kawata, S.; Masuhara, H., Vol.2. "Nanoplasmonics from Fundamentals to Applications", Chap. 8, 153-1842006

    • Author(s)
      Ayako Fujii, Akito Ishida
    • Total Pages
      31
    • Publisher
      Elsevier, Amsterdam
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] バイオセンシングのためのナノスペースプラズモン増強蛍光分析,プラズモンナノ材料の設計と応用技術,山田淳監修,第18章,239-2502006

    • Author(s)
      石田昭人, 藤井亜矢子
    • Total Pages
      11
    • Publisher
      シーエムシー出版
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Book] "Nanoplasmonics"、Handai Nanophotonics Series, Vol. 2, Elsevier, Amsterdam2006

    • Author(s)
      Ayako Fujii, Akito Ishida
    • Total Pages
      316
    • Publisher
      Elsevier, Amsterdam
    • Related Report
      2006 Annual Research Report
  • [Book] プラズモンナノ材料の設計と応用技術2006

    • Author(s)
      藤井亜矢子, 石田昭人
    • Total Pages
      340
    • Publisher
      シーエムシー出版
    • Related Report
      2006 Annual Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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