| Project/Area Number |
18510168
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
基礎ゲノム科学
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| Research Institution | Tokyo Medical and Dental University |
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Principal Investigator |
KOHDA Takashi Tokyo Medical and Dental University, 難治疾患研究所, Associate Professor (60211893)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,970,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥270,000)
Fiscal Year 2007: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2006: ¥2,800,000 (Direct Cost: ¥2,800,000)
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| Keywords | Somatic cell cloning / Epigenetics / Chromosomal domain / 遺伝子発現調節 / ゲノム / ゲノム領域 / 遺伝子発現調節単位 / 胎盤 |
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| Research Abstract |
Aim of this study is to analyze the epigenetic feature of the gene expression abnormalities in somatic cell cloning by nuclear transfer and the establishment of the methods to identified the genome-wide epigenetic transcriptional regulation domains through the analysis of gene expression profiles of the somatic cell cloned mice.
In this study, I propose a novel method to identify the gene expression domains with same expression pattern by calculating the Pearson's distance between the neighbouring genes on the chromosome. This method is easy way to identify gene cluster that is coregulated in the samples examined by DNA microarray with high throughput.
For the understanding of the basic nature of somatic cell done abnormalities, we examined the detailed histology of the clone placenta at the time point just after the implantation when most of the done embryos stop their development. It is also demonstrated that the gene expression change including genomic imprinted genes was induced in the done mice, not only in the neonatal tissue of doned mice, but also in the embryonic stem cells derived from cloned mouse embryos.
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