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Epigenetic regulation by HP1-SU(Z)12 molecular interaction

Research Project

Project/Area Number 18510172
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 基礎ゲノム科学
Research InstitutionKyushu University

Principal Investigator

YAMAMOTO Ken  Kyushu University, Medical Institute of Bioregulation, Associate Professor (60274528)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,210,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2006: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsSUZ12 / polycomb / MEP50 / EZH2 / E2F6 / histone H2A / transcription / PRC2 / ポリコーム / HP1 / heterochromatin / 転写制御 / ヒストンH2A
Research Abstract

SUZ12 is a Polycomb group protein that forms Polycomb repressive complexes (PRC2/3) together with EED and histone methyltransferase EZH2. Although the essential role of SUZ12 in regulating the activity of the PRC2/3 complexes has been demonstrated, additional function of this protein was suggested. Here, we show that SUZ12 interacts with WD-repeat protein MEP50 in vitro and in vivo. We show that the MEP50 binds histone H2A, selectively among core histones, and mediates transcriptional repression of protein arginine methyltransferase PRMT5, which is known to methylate H2A and H4. These results suggest that SUZ12 might have a role in transcriptional regulation through physical interaction with MEP50 that can be an adaptor between PRMT5 and its substrate H2A. In addition, we identified physical interaction between SUZ12-EZH2 complex and E2F6. This interaction suggests that SUZ12-EZH2 polycomb complex can be recruited onto genome through E2F6 to regulate transcription of its target genes. Because it has been shown that SUZ12 and EZH2 are up-regulated in cancer cells, our findings provide molecular target for cancer therapy.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (11 results)

All 2008 2007 2006 Other

All Journal Article (9 results) (of which Peer Reviewed: 4 results) Presentation (2 results)

  • [Journal Article] Onecut transcription factor OC2 is a direct target of T-bet in type-1 T-helper cells2008

    • Author(s)
      Furuno K., et. al.
    • Journal Title

      Genes Immun

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] K Onecut transcription factor OC2 is a direct target of T-bet in type-1 T-helper cells.2008

    • Author(s)
      Furuno K., et. al.
    • Journal Title

      Genes Immun. (In press)

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of HLA allele and killer immunoglobulin-like receptor ligand matching of clinical outcome in Leukemia patients undergoing transplantation with T-cell-replete marrow from an unrelated donor2007

    • Author(s)
      Morishima Y., et. al.
    • Journal Title

      Biol.Bood Marrow Transplant 13

      Pages: 315-328

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Effects of HLA allele and killer immunoglobulin-like receptor ligand matching on clinical outcome in Leukemia Patients undergoing transplantation with T-cell-replete marrow from an unrelated donor2007

    • Author(s)
      Morishima Y., et. al.
    • Journal Title

      Biol. Blood Marrow Transplant 13

      Pages: 315-328

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effects of HLA allele and killer immunoglobulin-like receptor ligand matching on clinical outcome in Leukemia Patients undergoing transplantation with T-cell-replete marrow from an unrelated donor.2007

    • Author(s)
      Morishima Y., et. al.
    • Journal Title

      Biol, Blood Marrow Transplant 13

      Pages: 315-328

    • Related Report
      2007 Annual Research Report
  • [Journal Article] Association of Polycomb group SUZ12 with WD-repeat protein MEP50 that binds to bistone H2A selectively in vitro2006

    • Author(s)
      Furuno K., et. al.
    • Journal Title

      Biochemical and Biophysical Research Communications 345

      Pages: 1051-1058

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Association of Polycomb group SUZ12 with WD-repeat protein MEP50 that binds to histone H2A selectively in vitro2006

    • Author(s)
      Furuno K., et. al.
    • Journal Title

      Biochemical and Biophysical Research Communications 345

      Pages: 1051-1058

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Association of Polycomb group SUZ12 with WD-repeat protein MEP50 that binds to histone H2A selectively in vitro.2006

    • Author(s)
      Furuno K. et al.
    • Journal Title

      Biochemical and Biophysical Research Communications 345

      Pages: 1051-1058

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Onecut transcription factor OC2 is a direct target of T-bet in type-1 T-helper cells

    • Author(s)
      Furuno K., et. al.
    • Journal Title

      Genes Immun (in press)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] E2F6転写制御に関わるSUZ12-EZH2複合体2006

    • Author(s)
      政次 俊宏, 他
    • Organizer
      第65回日本癌学会学術総会
    • Place of Presentation
      横浜
    • Year and Date
      2006-09-30
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] SUZ12-EZH2 complex regulates transcription activity of E2F62006

    • Author(s)
      Toshihiro Masatsugu, et. al.
    • Organizer
      The 65th Annual Meeting of Japanese Cancer Society
    • Place of Presentation
      Yokohama
    • Year and Date
      2006-09-30
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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