Investigation on plant intercellular chemical network using laser micro sampling technique

• Project/Area Number: 18510183
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Living organism molecular science
• Research Institution: Osaka University
• Principal Investigator: KAJIYAMA Shin'ichiro Osaka University, Graduate school of Engineering, Associate professor (Invited) (20243496)
• Project Period (FY): 2006 – 2007
• Project Status: Completed (Fiscal Year 2007)
• Budget Amount: ¥3,990,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥390,000); Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2006: ¥2,300,000 (Direct Cost: ¥2,300,000)
• Keywords: Single cell analysis / Cellular metabolite / Laser micro sampling / Nano Flow LC-MS / Chemical network / ArF excimer Laser / Laser cell-processing / Plant defense responses

Research Abstract

Unlike animal organs controlled by the central nervous system, multicellular plants axe composed of highly independent cells in functional and in metabolic aspect. This means metabolite analysis of whole plant or tissue samples only gives the average information of individual cell functions. For example, upon the infection of pathogens, some plants induce antimicrobial metabolite, phytoalexin. However the way and degree of defense response might not be same among individual cells. In this study, cellular metabolite accumulation and transcription of biosynthetic genes, among individual plant cells are investigated by using the technique of angle cell sampling and the analysis technique i.e. laser assisted single cell sampling, laser micro spectroscopy, and Nano Flow LC-ESI-MS. Through this study, we developed a novel experimental approach for functional cell biology of plants.

Research Products

• [Journal Article] Improvement of bioactive beads-mediated transformation by concomitant application of electroporation (2008)
  • Author(s): Murakawa, T., Kajiyama, S., Fukui, K.
  • Journal Title: Plant Biotechnology (印刷中)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Protection of isolated chromosomes using calcium-alginate microbeads(bioactive beads)toward chemical and physical stress. (2008)
  • Author(s): Murakawa, T., Hayashihara, K., Ito, K., Doi, T., Kajiyama, S., Fukui, K.
  • Journal Title: Chromosome Sci. (印刷中)
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Improvement of transformation efficiecy in bioactive beads-mediated gene transfer using DNA-lipofectin complex as entrapped genetic material (2008)
  • Author(s): Murakawa, T., Kajiyama, S., Ikeuchi, T., Kawakami, S., Fukui, K.
  • Journal Title: Journal of Bioscience and Bioengineering 105 Pages: 77-80
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Improvement of bioactive beads-mediated transformation by concomitant application of electroporation (2008)
  • Author(s): Murakawa, T., Kajiyama, S., Fukui, K
  • Journal Title: Plant Biotechnology (in printing)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Protection of isolated chromosomes using calcium-alginate microbeads(bioactive beads) toward chemical and physical stress (2008)
  • Author(s): Murakawa, T., Hayashihara, K., Ito, K., Doi, T., Kajiyama, S., Fukui, K
  • Journal Title: Chromosome Sci (in printing)
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Improvement of transformation efficiecy in bioactive beads-mediated gene transfer using DNA-lipofectin complex as entrapped genetic material (2008)
  • Author(s): Murakawa, T., Kajiyama, S., Ikeuchi, T., Kawakami, S., Fukui, K
  • Journal Title: J.Biosci.Bioeng 105 Pages: 77-80
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Improvement of transformation efficiecy in bioactive beads-mediated gene transfer using DNA-lipofectin complex as entrapped genetic material (2008)
  • Author(s): Murakawa, T., Kajiyama, S., Ikeuchi, T., Kawakami, S., Fukui, K.
  • Journal Title: Journal of Bioscience and Bioengineering (印刷中)
  • Peer Reviewed
• [Journal Article] Femtosecond Pulsed Laser as a Micro-Scalpel for Microdissection and Isolation of Specific Sections from Biological Samples (2007)
  • Author(s): Sakakura, M., Kajiyama, S., Tsutsumi, M., Si, J., Fukusaki, E., Tamaru, Y., Akiyama, S., Miura, K., Hirao, K., and Ueda, M.
  • Journal Title: Japanese Journal of Applied Physics 46 Pages: 5859-5864
  • NAID: 40015602604
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Novel plant transformation system by gene-coated gold particle introduction into specific cell using ArF excimer laser (2007)
  • Author(s): S. Kajiyama, F. Inoue, Y. Yoshikawa, T. Shoji, E. Fukusaki and A. Kobayashi
  • Journal Title: Plant Biotechnology 24 Pages: 315-320
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Highly sensitive spectral interferometric four-wave mixing microscopy near the shot noise limit and its combination with two-photon excited fluorescence microscopy (2007)
  • Author(s): Isobe, K., Ozeki, Y., Kawasumi, T., Kataoka, S., Kajiyama, S., Fukui, K., and Itoh
  • Journal Title: Opt. Express 14 Pages: 11204-11214
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Femtosecond Pulsed Laser as a Micro-Scalpel for Microdissection and Isolation of Specific Sections from Biological Samples (2007)
  • Author(s): Sakakura, M., Kajiyama, S., Tsutsumi, M., Si, J., Fukusaki, E., Tamaru, Y., Akiyama, S., Miura, K., Hirao, K., Ueda, M
  • Journal Title: Jap.J.Appl.Physics 46 Pages: 5859-5864
  • NAID: 40015602604
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Novel plant transformation system by gene-coated gold particle introduction into specific cell using ArF excimer laser (2007)
  • Author(s): Kajiyama, S., Inoue, F., Yoshikawa, Y., Shoji, T., Fukusaki, E., Kobayashi, A
  • Journal Title: Plant Biotechnology 24 Pages: 315-320
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] Novel plant transformation system by gene-coated gold particle introduction into specific cell using ArF excimer laser (2007)
  • Author(s): S.Kajiyama, F.Inoue, Y.Yoshikawa, T.Shoji, E.Fukusaki, A.Kobayashi
  • Journal Title: Plant Biotechnology (in press)(印刷中)
• [Journal Article] A. Single Cell-Based Analysis of Torenia Petal Pigments by a Combination of ArF Excimer Laser Micro Sampling and Nano-High Performance Liquid Chromato graphy(HPLC)-Mass Spectrometry (2006)
  • Author(s): Kajiyama, S., Harada, K., Fukusaki, E., and Kobayashi, A
  • Journal Title: J. Biosci. Bioeng. 106 Pages: 575-578
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] A Novel Microsurgery Method for Intact Plant Tissue at the Single Cell Level using ArF Excimer Laser Microprojection (2006)
  • Author(s): Kajiyama, S., Shoji, T., Okuda, S., Izumi, Y., Fukusaki, E., and Kobayashi, A.
  • Journal Title: Biotechnology and Bioengineering 93 Pages: 325-331
  • Description: 「研究成果報告書概要(和文)」より
  • Peer Reviewed
• [Journal Article] Highly sensitive spectral interferometric four-wave mixing microscopy near the shot noise limit and its combination with two-photon excited fluorescence microscopy (2006)
  • Author(s): Isobe, K., Ozeki, Y., Kawasumi, T, Kataoka, S., Kajiyama, S., Fukui, K., Itoh
  • Journal Title: Opt. Express 14 Pages: 11204-11214
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A.Single Cell-Based Analysis of Torenia Petal Pigments by a Combination of ArF Excimer Laser Micro Sampling and Nano-High Performance Liquid Chromatography (HPLC)-Mass Spectrometry (2006)
  • Author(s): Kajiyama, S., Harada, K., Fukusaki, E., Kobayashi, A
  • Journal Title: J. Biosci. Bioeng 102(6) Pages: 575-578
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A Novel Microsurgery Method for Intact Plant Tissue at the Single Cell Level using ArF Excimer Laser Microprojection (2006)
  • Author(s): Kajiyama, S., Shoji, T., Okuda, S., Izumi, Y., Fukusaki, E., Kobayashi, A
  • Journal Title: Biotech.and Bioeng 93(2) Pages: 325-331
  • Description: 「研究成果報告書概要(欧文)」より
• [Journal Article] A novel microsurgery method for intact plant tissue at the single cell level using ArF excimer laser microprojection (2006)
  • Author(s): S.Kajiyama, T.Shoji, S.Okuda, Y.Izumi, E.Fukusaki, A.Kobayashi
  • Journal Title: Biotechnology and Bioengineering 93(2) Pages: 325-331
• [Journal Article] Single cell-based analysis of Torenia petal pigments by a combination of ArF excimer laser micro sampling and nano-HPLC-Mass spectrometry (2006)
  • Author(s): S.Kajiyama, K.Harada, E.Fukusaki, A.Kobayashi
  • Journal Title: Journal of Bioscience and Bioengineering 102(6) Pages: 575-578
• [Journal Article] Highly sensitive spectral interferometric four-wave mixing microscopy near the shot noise limit and its combination with two-photon excited fluorescence microscopy (2006)
  • Author(s): K.Isobe, Y.Ozeki, T.Kawasumi, S.Kataoka, S.Kajiyama, K.Fukui, K.Itoh
  • Journal Title: Opt. Express 14 Pages: 11204-11214
• [Presentation] Single cell sampling and analysis by ultra short pulse laser (2007)
  • Author(s): Kajiyama, S., Sakakura, M., Ueda, M., Itoh, K., and Fukui, K.
  • Organizer: 5th International forum of post genome technologies
  • Place of Presentation: Bamboo Grove Hotel, Suzhou, China
  • Year and Date: 2007-09-11
• [Presentation] 動植物の組織・細胞単離のためのフェムト秒レーザー加工法 (2007)
  • Author(s): 坂倉政明, 司 金海, 梶山慎一郎, 平尾一之, 栃木憲治, 植田充美
  • Organizer: 第68回日本応用物理学会
  • Place of Presentation: 北海道工業大学
  • Year and Date: 2007-09-08
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Laser processing for plant and animal tissues (2007)
  • Author(s): Sakakura, M., Shi, K., Kajiyama, S, Hirao, K., Tbchigi, K., Ueda, M
  • Organizer: 68th Conference of Japanese Society of Applied Physics
  • Place of Presentation: Hokkaido. Japan
  • Year and Date: 2007-09-08
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Single cell sampling and analysis by ultra short pulse laser (2007)
  • Author(s): Kajiyama, S., Sakakura, M., Ueda, M., Itoh, K., and Fukui, K.
  • Organizer: 5th International forum of post genome technologies(5'IFPT)
  • Place of Presentation: Shuzou, China
  • Year and Date: 2007-09-07
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Single cell sampling and analysis by ultra short pulse laser (2007)
  • Author(s): Kajiyama, S., Sakakura, M., Ueda, M., Itoh, K., Fukui, K
  • Organizer: 5th International forum of post genome technologies (5'IFPT)
  • Place of Presentation: Shuzou, China
  • Year and Date: 2007-09-07
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 酸化ストレス条件下でのエラープローンなDNA polymeraseの発現解析 (2007)
  • Author(s): 平野 美紀、梶山 慎一郎、栗原 佐智子、福井 希一
  • Organizer: 日本農芸化学会2007年度大会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2007-03-26
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] シングルセル代謝分析に基づいた植物感染応答反応の動的解析 (2007)
  • Author(s): 和泉 自泰、福崎 英一郎、梶山 慎一郎、兼松 泰男、中村 亮介、石原 亨、小林 昭雄
  • Organizer: 日本農芸化学会2007年度大会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2007-03-26
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Expression analysis of error-prone DNA polymerase under oxdative stress (2007)
  • Author(s): Hirano, M., Kajiyama, S., Kurihara, S., Fukui, K
  • Organizer: Annual conference of Japanese society for bioscience,biotechnology, and agrochemistry
  • Place of Presentation: Tbkyo, Japan
  • Year and Date: 2007-03-26
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Dynamic analysis of plant defence responces by single cell sampling (2007)
  • Author(s): Izumi, Y., Fukusaki, E., Kajiyama, S., Kanematsu, Y., Nakamura, R., Ishihara, R., Kobayashi, A
  • Organizer: Annual conference of Japanese society for bioscience, biotechnology, and agrochemistry
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2007-03-26
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Single-cell based metabolite analyses by laser microsampling and nano-flow LC-MS (2006)
  • Author(s): Kajiyama, S.
  • Organizer: 4th International forum on post-genome technologies(4'IFPT)
  • Place of Presentation: Hanzou, China
  • Year and Date: 2006-09-25
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Single-cell based metabolite analyses by laser microsampling and nano-flow LC-MS (2006)
  • Author(s): Kajiyama, S
  • Organizer: 4th International forum on post-genome technologies (4' IFPM)
  • Place of Presentation: Hanzou, China
  • Year and Date: 2006-09-25
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] 質量分析による転写産物の新規定量法の開発 (2006)
  • Author(s): 広実 慶彦, 福崎 英一郎, 梶山 慎一郎, 小林 昭雄
  • Organizer: 日本生物工学会2006年度大会
  • Place of Presentation: 大阪大学豊中キャンパス
  • Year and Date: 2006-09-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] 1細胞分析に資するレーザーテクノロジー (2006)
  • Organizer: 日本生物工学会2006年度大会シンポジウム
  • Place of Presentation: 大阪大学豊中キャンパス
  • Year and Date: 2006-09-11
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Novel quantitative analysis method for m-RNA by mass spectrometry (2006)
  • Author(s): Hirozane, Y., Fukusaki, E., Kajiyama, S., Kobayashi, A
  • Organizer: Annual conference of Japanese society for bioscience and bioengineering
  • Place of Presentation: Osaka, Japan
  • Year and Date: 2006-09-11
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] Laser technology for single cell analysis (2006)
  • Author(s): Kajiyama, S
  • Organizer: Annual conference of Japanese society for bioscience and bioengineering
  • Place of Presentation: Osaka, Japan
  • Year and Date: 2006-09-11
  • Description: 「研究成果報告書概要(欧文)」より
• [Presentation] フェムト秒レーザーを用いた細胞のサンプリングと分析 (2006)
  • Author(s): 梶山慎一郎
  • Organizer: 日本分光学会関西支部大会シンポジウム
  • Place of Presentation: JSTイノベーションプラザ京都
  • Year and Date: 2006-06-29
  • Description: 「研究成果報告書概要(和文)」より
• [Presentation] Cellular sampling and analysis by femto second laser (2006)
  • Author(s): Kajiyama, S
  • Organizer: Symposium of Japanese society for spectra analysis (Kansai area)
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2006-06-29
  • Description: 「研究成果報告書概要(欧文)」より
• [Book] 共同執筆 ナノイメージング 「超短誘導パラメトリック発光を利用した蛍光染色を必要としない生細胞の観察」 (2008)
  • Author(s): 梶山慎一郎、小関泰之、福井希一、伊東一良(分担執筆)
  • Publisher: NTS(株)(印刷中)
  • Description: 「研究成果報告書概要(和文)」より
• [Book] ナノイメージング (2008)
  • Author(s): 梶山慎一郎(分担執筆)
  • Publisher: NTS(出版確定)
• [Book] 山根恒夫、松永是、民谷栄一 監修ナノバイオ大辞典「フェムト秒レーザー」 (2007)
  • Author(s): 梶山慎一郎(分担執筆)
  • Total Pages: 650
  • Publisher: (株)テクノシステム
  • Description: 「研究成果報告書概要(和文)」より
• [Book] ナノバイオ大辞典 (2007)
  • Author(s): 山根恒夫, 松永是, 民谷栄一 監修 梶山慎一郎(執筆分担)
  • Total Pages: 636
  • Publisher: 株式会社 テクノシステム
• [Patent(Industrial Property Rights)] PCRにより増幅された伸長DNA鎖の検出方法、および、標的配列の測定方法 (2006)
  • Inventor(s): 梶山慎一郎、福崎英一郎、広実慶彦、梨原善行
  • Industrial Property Rights Holder: 大阪大学, 他
  • Industrial Property Number: 2006-346582
  • Filing Date: 2006-12-22
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] 形質転換体の製造方法及び酵母の新規変異株 (2006)
  • Inventor(s): 植田 充美、平尾 一之、梶山慎一郎、福崎英一郎、司 金海、秦 洋二、堤 浩子
  • Industrial Property Rights Holder: 大阪大学, 他
  • Industrial Property Number: 2006-346522
  • Acquisition Date: 2006-12-22
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] レーザ加工装置およびレーザ加工方法 (2006)
  • Inventor(s): 植田 充美、平尾 一之、梶山慎一郎、坂倉 政明、ドゥミトリトゥジリン、ザポロシェンコユーリ、島田竜太郎
  • Industrial Property Rights Holder: 大阪大学, 他
  • Industrial Property Number: 2006-280135
  • Acquisition Date: 2006-10-30
  • Description: 「研究成果報告書概要(和文)」より
• [Patent(Industrial Property Rights)] PCRにより増幅された伸長DNA鎖の検出方法、および、標的配列の測定方法 (2006)
  • Inventor(s): 梶山慎一郎, 福崎英一郎, 梨原 善行, 広実 慶彦
  • Industrial Property Rights Holder: 大阪大学
  • Industrial Property Number: 2006-346582
  • Filing Date: 2006-12-22