| Project/Area Number |
18510183
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Osaka University |
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Principal Investigator |
KAJIYAMA Shin'ichiro Osaka University, Graduate school of Engineering, Associate professor (Invited) (20243496)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
|
| Budget Amount *help |
¥3,990,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | Single cell analysis / Cellular metabolite / Laser micro sampling / Nano Flow LC-MS / Chemical network / ArF excimer Laser / Laser cell-processing / Plant defense responses |
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| Research Abstract |
Unlike animal organs controlled by the central nervous system, multicellular plants axe composed of highly independent cells in functional and in metabolic aspect. This means metabolite analysis of whole plant or tissue samples only gives the average information of individual cell functions. For example, upon the infection of pathogens, some plants induce antimicrobial metabolite, phytoalexin. However the way and degree of defense response might not be same among individual cells. In this study, cellular metabolite accumulation and transcription of biosynthetic genes, among individual plant cells are investigated by using the technique of angle cell sampling and the analysis technique i.e. laser assisted single cell sampling, laser micro spectroscopy, and Nano Flow LC-ESI-MS. Through this study, we developed a novel experimental approach for functional cell biology of plants.
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