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Identification and topology analysis of plant odorant receptors

Research Project

Project/Area Number 18510186
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Living organism molecular science
Research InstitutionHiroshima University

Principal Investigator

IZUMI Shunsuke  Hiroshima University, Graduate School of Science, Associate Professor (90203116)

Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,000,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥2,700,000 (Direct Cost: ¥2,700,000)
KeywordsOdorant receptors / Proteomics / Biotransformation / Membrane topology / Chemical modification / Mass spectrometry / 高等植物 / モノテルペノイド
Research Abstract

Volatile substances and their receptors was involved in plant-plant communications.
We have discussed the roles of terpenes, green odor, Me jasmonate, and plant GPCR (G protein-coupled receptors), two-component system, and chemoreceptor channels as candidates for plant odorant receptors.
Next, Protein clusters rule the stereoselectivity of pulegone reductase; the pulegone reductase which bound with β-glucosidase and trypsin inhibitor catalyzed (1R,4R)-isomenthone preferentially rather than (1R,4S)-menthone from (R)-pulegone.
Furthermore, the topology and the dynamics of peptide within the liposomes were investigated by use of mass spectrometry combined with acetylation. The peptide within theliposomes was acetylated with NHSSAc; the N-terminal of melittin was acetylated. Thus, it was supposed that the peptide within the liposomes adopted an N-terminal topology.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (17 results)

All 2008 2007 2006

All Journal Article (15 results) (of which Peer Reviewed: 6 results) Presentation (2 results)

  • [Journal Article] GSK-3β Regulates Proper Mitotic Spindle Formation in Cooperation with a Component of the γ -Tubulin Ring Complex, GCP5.2008

    • Author(s)
      Izumi, Nanae, Fumoto, Katsumi, Izumi, Shunsuke, Kikuchi, Akira.
    • Journal Title

      Journal of Biological Chemistry 283(19)

      Pages: 12981-12991

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] GSK-3P Regulates Proper Mitotic Spindle Formation in Cooperation with a Component of the y -Tubulin Ring Complex, GCP5.2008

    • Author(s)
      Izumi, Nanae, Fumoto, Katsumi, Izumi, Shunsuke, Kikuchi, Akira
    • Journal Title

      J. Biol. Chem. 283

      Pages: 12981-12991

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Nucleotide excision repair and homologous recombination systems commit differentially to the repair of DNA-protein crosslinks.2007

    • Author(s)
      Nakano, Toshiaki, Morishlta, Soh, Katafuchi, Atsushi, Matsubara, Mayumi, Horikawa, Yusuke, Terato, Hiroak, Salem, Amir M. H., Izumi, Shunsuke, Pack, Seung Pil, Makino, Keisuke, Ide, Hiroshi.
    • Journal Title

      Molecular Cell 28(1)

      Pages: 147-158

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Nucleotide excision repair and homologous recombination systems commit differentially to the repair of DNA-protein crosslinks.2007

    • Author(s)
      Nakano, Toshiaki, Morishita, Soh, Katafuchi, Atsushi, Matsubara, Mayumi, Horikawa, Yusuke, Terato, Hiroaki, Salem, Amir M. H., Izumi, Shunsuke, Pack, Seung Pil, Makino, Keisuke, Ide, Hiroshi
    • Journal Title

      Molecular Cell 28

      Pages: 147-158

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Mass spectrometry of hydrogen/deuterium exchange in 70S ribosomal proteins from E. coli.2007

    • Author(s)
      Yamamoto, Tatsuya, Izumi, Shunsuke, Gekko, Kunihiko
    • Journal Title

      FEBS Letters 580

      Pages: 3638-3642

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] DNA Damage-Dependent Acetylation and Ubiquitination of H2AX Enhances Chromatin Dynamics.2007

    • Author(s)
      T. Ikura, S. Tashiro, A. Kakino, H. Shima, N. Jacob, R. Amunugama, K. Yoder S. Izumi,
    • Journal Title

      Mol. Cell. Biol., 27

      Pages: 7028-7028

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Topological study of membrane proteins by mass spectrometry.2007

    • Author(s)
      H. Mizuno, S. Izumi, K. Kominami, and T. Hirata
    • Journal Title

      J. Mass Spec. Soc. Jpn., 55

      Pages: 221-221

    • NAID

      10019487591

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Post-translational palmitoylation and glycosylation of wnt-5a are necessary for its signalling.2007

    • Author(s)
      M. Kurayoshi, H. Yamamoto, S. Izumi, and A. Kikuchi
    • Journal Title

      Biochem. J., 402

      Pages: 515-515

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Post-translational palmitoylation and glycosylation of Wnt-5a are necessary for its signalling.2007

    • Author(s)
      Kurayoshi, Manabu, Yamamoto, Hideki, Izumi, Shunsuke, Kikuchi, Akira.
    • Journal Title

      Biochemical Journal 402(3),

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Allosteric inhibition of rat neuronal nitric-oxide synthase caused by interference with the binding of calmodulin to the enzyme.2007

    • Author(s)
      Ohashi, Koji, Yamazaki, Takeshi, Kitamura, Shigeyuki, Ohta, Shigeru, Izumi, Shunsuke, Kominami, Shiro.
    • Journal Title

      Biochimica et Biophysica Acta, 1770(2)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Mass spectrometry of hydrogen/deuterium exchange in 70S ribosomal proteins from E. coli.2006

    • Author(s)
      Yamamoto, Tatsuya, Izum i, Shunsuke, Gekko, Kunihiko.
    • Journal Title

      FEBS Letters 580

      Pages: 3638-3642

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Mitochondrial processing of bovine adrenal steroidogenic acute regulatory protein.2006

    • Author(s)
      Yamazaki, Takeshi, Matsuoka, Chisa, Gendou, Miho, Izumi, Shunsuke, Zhao, Dong, Artemenko, Irina, Jefcoate, Colin R., Kominami, Shiro.
    • Journal Title

      Biochimica et Biophysica Acta, Proteins and Proteomics 1764(10),

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Characterization of rat and human CYP2J enzymes as Vitamin D 25-hydroxylases.2006

    • Author(s)
      Aiba, Isamu, Yamasaki, Tomoaki, Shinki, Toshimasa, Izumi, Shunsuke, Yamamoto, Keiko, Yamada, Sachiko, Terato, Hiroaki, Ide, Hiroshi, Ohyama, Yoshihiko.
    • Journal Title

      Steroids 71(10),

    • Related Report
      2006 Annual Research Report
  • [Journal Article] High glucose concentration induces elevated expression of anti-oxidant and proteolytic enzymes in cultural human retinal pigment epithelial cells.2006

    • Author(s)
      Yokoyama, Tomoko, Yamane, Ken, Minamoto, Atsushi, Tsukamoto, Hidetoshi, Yamashita, Hidetoshi, Izumi, Shunsuke, Hoppe, George, Sears, Jonathan E., Mishima, Hiromu K.
    • Journal Title

      Experimental Eye Research 83(3)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Mass spectrometry of hydrogen/deuterium exchange in 70S ribosomal proteins from E. coli.2006

    • Author(s)
      Yamamoto, Tatsuya, Izumi, Shunsuke, Gekko, Kunihiko.
    • Journal Title

      FEBS Letters 580(15)

      Pages: 3638-3642

    • Related Report
      2006 Annual Research Report
  • [Presentation] Mechanism of the Insertion into Bilayer Membrane by Melittin2007

    • Author(s)
      K. Saikusa, S. Izumi, T. Hirata
    • Organizer
      55th ASMS Conference
    • Place of Presentation
      Indianapolis
    • Year and Date
      2007-06-14
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Mechanism of the Insertion into BilayerMembrane by Melittin2007

    • Author(s)
      K. Saikusa, S. Izumi, T. Hirata
    • Organizer
      55th ASMS Conference
    • Place of Presentation
      Indianapolis, USA
    • Year and Date
      2007-06-14
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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