Identification and topology analysis of plant odorant receptors
| Project/Area Number |
18510186
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Hiroshima University |
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Principal Investigator |
IZUMI Shunsuke Hiroshima University, Graduate School of Science, Associate Professor (90203116)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥4,000,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥2,700,000 (Direct Cost: ¥2,700,000)
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| Keywords | Odorant receptors / Proteomics / Biotransformation / Membrane topology / Chemical modification / Mass spectrometry / 高等植物 / モノテルペノイド |
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| Research Abstract |
Volatile substances and their receptors was involved in plant-plant communications.
We have discussed the roles of terpenes, green odor, Me jasmonate, and plant GPCR (G protein-coupled receptors), two-component system, and chemoreceptor channels as candidates for plant odorant receptors.
Next, Protein clusters rule the stereoselectivity of pulegone reductase; the pulegone reductase which bound with β-glucosidase and trypsin inhibitor catalyzed (1R,4R)-isomenthone preferentially rather than (1R,4S)-menthone from (R)-pulegone.
Furthermore, the topology and the dynamics of peptide within the liposomes were investigated by use of mass spectrometry combined with acetylation. The peptide within theliposomes was acetylated with NHSSAc; the N-terminal of melittin was acetylated. Thus, it was supposed that the peptide within the liposomes adopted an N-terminal topology.
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Report
(3 results)
Research Products
(17 results)
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[Journal Article] Nucleotide excision repair and homologous recombination systems commit differentially to the repair of DNA-protein crosslinks.2007
Author(s)
Nakano, Toshiaki, Morishlta, Soh, Katafuchi, Atsushi, Matsubara, Mayumi, Horikawa, Yusuke, Terato, Hiroak, Salem, Amir M. H., Izumi, Shunsuke, Pack, Seung Pil, Makino, Keisuke, Ide, Hiroshi.
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Journal Title
Molecular Cell 28(1)
Pages: 147-158
Description
「研究成果報告書概要(和文)」より
Related Report
Peer Reviewed
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[Journal Article] Nucleotide excision repair and homologous recombination systems commit differentially to the repair of DNA-protein crosslinks.2007
Author(s)
Nakano, Toshiaki, Morishita, Soh, Katafuchi, Atsushi, Matsubara, Mayumi, Horikawa, Yusuke, Terato, Hiroaki, Salem, Amir M. H., Izumi, Shunsuke, Pack, Seung Pil, Makino, Keisuke, Ide, Hiroshi
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Journal Title
Molecular Cell 28
Pages: 147-158
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Allosteric inhibition of rat neuronal nitric-oxide synthase caused by interference with the binding of calmodulin to the enzyme.2007
Author(s)
Ohashi, Koji, Yamazaki, Takeshi, Kitamura, Shigeyuki, Ohta, Shigeru, Izumi, Shunsuke, Kominami, Shiro.
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Journal Title
Biochimica et Biophysica Acta, 1770(2)
Related Report
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[Journal Article] Mitochondrial processing of bovine adrenal steroidogenic acute regulatory protein.2006
Author(s)
Yamazaki, Takeshi, Matsuoka, Chisa, Gendou, Miho, Izumi, Shunsuke, Zhao, Dong, Artemenko, Irina, Jefcoate, Colin R., Kominami, Shiro.
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Journal Title
Biochimica et Biophysica Acta, Proteins and Proteomics 1764(10),
Related Report
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[Journal Article] Characterization of rat and human CYP2J enzymes as Vitamin D 25-hydroxylases.2006
Author(s)
Aiba, Isamu, Yamasaki, Tomoaki, Shinki, Toshimasa, Izumi, Shunsuke, Yamamoto, Keiko, Yamada, Sachiko, Terato, Hiroaki, Ide, Hiroshi, Ohyama, Yoshihiko.
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Journal Title
Related Report
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[Journal Article] High glucose concentration induces elevated expression of anti-oxidant and proteolytic enzymes in cultural human retinal pigment epithelial cells.2006
Author(s)
Yokoyama, Tomoko, Yamane, Ken, Minamoto, Atsushi, Tsukamoto, Hidetoshi, Yamashita, Hidetoshi, Izumi, Shunsuke, Hoppe, George, Sears, Jonathan E., Mishima, Hiromu K.
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Journal Title
Experimental Eye Research 83(3)
Related Report
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