Research of new medicinal compounds against the osteoporosis from the marine echinoderms.
| Project/Area Number |
18510187
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Kyushu University |
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Principal Investigator |
INAGAKI Masanori Kyushu University, Faculty of Pharmaceutical Sciences, Assistant Professor (90274480)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,960,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2006: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | Sphingolipid / Echinodermata / Starfish / Sea Cucumber / Feather Star / Osteoporosis / セラミド / ガングリオシド |
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| Research Abstract |
We have found that the glycosphingolipid molecular species from marine echinoderms possess anti-osteoporosis effects toward the osteoporosis model mice (OVX mice) by osteoclast colony assay and osteogenetic cell colony assay. Continuing our research for anti-osteoporosis compounds from echinoderms, we accomplished the following studies in these years.
The echinoderms used as materials were as follows. The starfish Linckia laevigata (collected at Okinawa) and Certonardoa semiregularis (collected at Fukuoka), sea cucumber Bohadschia argus, Thelenota ananas (each collected at Okinawa), and Stichopus japonicus (collected at the Inland Sea), and feather star Comanthina schlegeli (collected at Okinawa). These animals were extracted with CHC13/MeOH and the extracts were partitioned between water and organic solvent, and each fractions were purified by normal phase and reversed phase column chromatography. On the basis of chemical and spectroscopic evidences, we determined a new ganglioside molecular species from L. laevigata, a new ganglioside molecular species from S. japonicus, two new inositol-phosphoceramide type ganglioside molecular species from C. schlegeli, three glucocerebroside molecular species from T. ananas, two glucocerebroside molecular species from C. semiregularis and a galactocerebroside molecular species from B. argus. Galactocerebroside molecular species were further separated by reversed phase HPLC in a difference of the length of alkyl chain, to give the individual components of molecular species.
These sphingolipids obtained in this research are very useful for examination of structure activity relationship of the sphingolipid that the effectiveness was seen toward the OVX mice.
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Report
(3 results)
Research Products
(45 results)
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[Journal Article] Constituents of Holothuroidea, 17. Isolation and Structure of Biologically Active Monosialo-Gangliosides from the Sea Cucumber Cucumaria echinata2006
Author(s)
F., Kisa, K., Yamada, T., Miyamoto, M., Inagaki, R., Higuchi
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Journal Title
Chem. Pharm. Bull. 54(7)
Pages: 982-987
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Constituents of Holothuroidea, 18. Isolation and Structure of Biologically Active Disialo and Trisialo-Gangliosides from the Sea Cucumber Cucumaria echinata2006
Author(s)
F., Kisa, K., Yamada, T., Miyamoto, M., Inagaki, R., Higuchi
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Journal Title
Chem. Pharm. Bull. 54(9)
Pages: 1293-1298
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Journal Article] Isolation and Structure of Four New Ceramides from the Starfish Luidia maculata2006
Author(s)
M., Inagaki, Y., Ikeda, S., Kawatake, K., Nakamura, M., Tanaka, E., Misawa, M., Yamada, R., Higuchi
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Journal Title
Chem. Pharm. Bull. 54(12)
Pages: 1647-1649
NAID
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Langmuir Monolayers of Cerebroside Originated from Bohadschia argus with Different Sugars at the Air-Water interface2007
Author(s)
Y., Ikeda, M., Inagaki, B., Zhang, K., Yamada, R., Higuchi, O., Shibata
Organizer
The 46th Annual Meeting of Japan Oil Chemists' Society
Place of Presentation
Kyoto
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Behavior of Cerebrosides Langmuir Monolayer with Different Sugars at the Air-Water interface2007
Author(s)
Y., Ikeda, M., Inagaki, B., Zhang, K., Yamada, R., Higuchi, O., Shibata
Organizer
The 60th Divisional Meeting on Colloid and Interface Chemistry
Place of Presentation
Matsumoto
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Behavior of Cerebroside Langmuir Monolayers Originated from the Echinoderms with Different Sugars at the Air-Water interface2007
Author(s)
Y., Ikeda, M., Inagaki, B., Zhang, K., Yamada, R., Higuchi, O., Shibata
Organizer
The 24th Annual Meeting of Japan Pharmaceutical Society Kyushu Branch
Place of Presentation
Fukuoka
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Isolation and Structure of Phosphoglycosphingolipids from the Okinawan Feather Stars, Comanthina schlegeli and Comanthus bennetti2006
Author(s)
M., Inagaki, M., Shiizaki, D., Yatsugi, T., Miyamoto, R., Higuchi
Organizer
25th IUPAC International Conference on Biodiversity and Natural Products
Place of Presentation
Kyoto
Description
「研究成果報告書概要(欧文)」より
Related Report
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[Presentation] Isolation and Structure of Phosphoglycosphingolipids from the Okinawan Feather Star Comanthina schlegeli2006
Author(s)
M., Inagaki, M., Shiizaki, T., Hiwatashi, T., Miyamoto, R., Higuchi
Organizer
2006' International Symposium for Pharmaceutical Sciences in Beijing
Place of Presentation
Beijing, China
Description
「研究成果報告書概要(欧文)」より
Related Report
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