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Development and application of assay systems for searching antipruritic drug

Research Project

Project/Area Number 18510196
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Living organism molecular science
Research InstitutionMukogawa Women's University

Principal Investigator

OKU Hisae  Mukogawa Women's University, School of Pharmacy and Pharmaceutical Sciences, Assistant (90281518)

Co-Investigator(Kenkyū-buntansha) ISHIGURO Kyoko  Mukogawa Women's University, School of Pharmacy and Pharmaceutical Sciences, Professor (70151363)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥3,880,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥180,000)
Fiscal Year 2007: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2006: ¥3,100,000 (Direct Cost: ¥3,100,000)
Keywordsbioactive / Pharmacy / animal model of disease / itch / Natural resources / stress
Research Abstract

The following report describes our research using the mouse pruritis-model to investigate the factors that trigger pruritis and contribute to its worsening. Moreover, we also extended our research to anti-pruritics as an effort to discover new itch-suppression materials from natural resources which are essential in treatment of atopic dermatitis and other skin inflammations.
1. Central nervous system mechanisms present in the model for transient pruritis have been observed in the NC mice of atopic dermatitis model. This model was used for atopic dermatitis in an effort to create a model that exhibits worsening of pruritis in response to stress. In order to quantify the stress level in mice, biochemistry of blood was performed to measure the concentrations of corticosterone, a stress hormone. Then, using a communications box purchased out of the equipment budget for this application, we applied random direct shocks to induce stress in mice, creating easily replicable stress conditions within a short span of time. Consequently, we also investigated the correlation and relationship between the degree of stress and the scratching behaviour in vivo.
2. We used a variety of assay methods to create an index for measuring the frequency of scratching for degranulating compound 48/80, serotonin and platelet activating factor, and protease, which specifically increase at the site of inflammation. In our search for seeds from natural resources, containing anti-pruritic properties we discovered that the bud of the white Rose of Sharon, obtained from the Shrubalthea Flower, Chinese medicine was effective, we investigated the effectiveness of different varieties of Rose of Sharon, and observed that the red buds were more effective against allergic pruritis than the white buds used in Chinese medicine. Moreover, we were also able to clarify that the active ingredients consisted of several varieties of flavonol and anthocyan derivatives.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (46 results)

All 2008 2007 2006

All Journal Article (25 results) (of which Peer Reviewed: 12 results) Presentation (21 results)

  • [Journal Article] Involvement of inducible Nitric Oxide Synthase in Blood Decrease in vein induced by Hen-egg White Lysozyme2007

    • Author(s)
      Oku, H., Iwaoka, E., Ogawa, Y., Ishiguro, K.
    • Journal Title

      Biol.Pharm.Bull. 30

      Pages: 1324-1328

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Allergy-preventive Phenolic Glycoside Xanthorrhoea hastilis2007

    • Author(s)
      Ogawa, Y., Oku, H., Iwaoka, E., Iinuma, M.and Ishiguro, K.
    • Journal Title

      Chem.Pharm.Bull. 55

      Pages: 675-678

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Development of in vivo Bioassay method for preimary preventive substances of the metabolic syndrome-Effect of Kinginka-cha-2007

    • Author(s)
      Oku, H., Iwaoka, E., Ogawa, Y., Ishiguro, K.
    • Journal Title

      Clical Exp.Pharmaco.Physiol 34

      Pages: 40-42

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Primary preventive teas on metabolic syndrome-mechanisms of Kinginkacha and Kinkacha-2007

    • Author(s)
      Oku, H., Ogawa, Y., Iwaoka, E., Yamaguchi, Y., Kunitomo, M. and Ishiguro, K.
    • Journal Title

      Yakugaku Zasshi 127

      Pages: 40-42

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Involvement of inducible Nitric Oxide Synthase in Blood Decrease in vein induced by Hen-egg White Lysozyme.2007

    • Author(s)
      Oku. H., Iwaoka, E., Ogawa, Y., Ishiguro, K.
    • Journal Title

      Biol. Pharm. Bull. 30

      Pages: 1324-1328

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Allergy-preventive Phenolic Glycoside Xanthorrhoea hastilis2007

    • Author(s)
      Ogawa, Y., Oku. H., Iwaoka, E., Iinuma, M, Ishiguro, K.
    • Journal Title

      Chem. Pharm. Bull. 55

      Pages: 675-678

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Development of in vivo Bioassay method for preimary preventive substances of the metabolic syndrome-Effect of Kinginka-cha-2007

    • Author(s)
      Oku, H., Iwaoka, E., Ogawa, Y., Ishiguro, K.
    • Journal Title

      Clical Exp. Pharmaco. Physiol. 34

      Pages: 40-42

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Primary preventive teas on metabolic syndrome-mechanisms of Kinginkacha and Kinkacha-2007

    • Author(s)
      Oku, H., Ogawa, Y., Iwaoka, E., Yamaguchi, Y., Kunitomo, M., Ishiguro, K.
    • Journal Title

      Yakugaku Zasshi 127

      Pages: 40-42

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Involvement of inducible Nitric Oxide Synthase in Blood Decrease in ve in induced by Hen-egg White Lvsozvme2007

    • Author(s)
      Oku, H., Iwaoka, E., Ogawa, Y., Ishiguro, K.
    • Journal Title

      Biol.Pharm.Bull 30

      Pages: 1324-1328

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Allergy-preventive Phenolic Glycoside Xanthorrhoea hastilis2007

    • Author(s)
      Ogawa, Y., Oku, H., Iwaoka, E., Iinuma, M, Ishiguro, K.
    • Journal Title

      Chem.Pharm.Bull 55

      Pages: 675-678

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of in vivo Bioassay method for preimary preventive substances of the metabolic syndrome-Effect of Kinginka-cha2007

    • Author(s)
      Oku, H., Iwaoka, E., Ogawa, Y., Ishiguro, K.
    • Journal Title

      Clical Exp.Pharmaco.Physiol 34

      Pages: 40-42

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Primary preventive teas on metabolica syndrome-mechanisms of Kinginkach a and Kinkacha-2007

    • Author(s)
      Oku, H., Ogawa, Y., Iwaoka, E., Yamaguchi, Y., Kunitomo, M., Ishiguro, K.
    • Journal Title

      Yakugaku Zasshi 127

      Pages: 40-42

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] allergy-preventive Phenolic Glycoside Xanthorrhoea hastilis2007

    • Author(s)
      Ogawa, Y., Oku, H., Iwaoka, E., Iinuma, M, Ishiguro, K.
    • Journal Title

      Chem.Pharm.Bull. (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Involvement of inducible Nitric Oxide Synthase in Blood Decrease in vein induced by Hen-eff White Lysozyme.2007

    • Author(s)
      Oku, H., Iwaoka, E., Ogawa, Y., Ishiguro, K.
    • Journal Title

      Biol.Pharm.Bull. (in press)

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Development of an in vivo bioassay method for primary preventive substances of the metabolic syndrome-Effect of Kinginkacha-2006

    • Author(s)
      Oku, H., Ogawa, Y., Iwaoka, E. and Ishiguro, K.
    • Journal Title

      Yakugaku Zasshi 126

      Pages: 158-160

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Primary preventive teas on metabolic syndrome-Kinginkacha,kinkacha and kudingcha-2006

    • Author(s)
      Oku, H., Ogawa, Y., Iwaoka, E. and Ishiguro, K.
    • Journal Title

      Yakugaku Zasshi 126

      Pages: 114-115

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Allergy-preventive Phenolic Glycoside from Populus sieboldii2006

    • Author(s)
      Ogawa, Y., Oku, H., Iwaoka, E., Iinuma, M., and Ishiguro, K.
    • Journal Title

      J.Nat.Prod. 69

      Pages: 1215-1217

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Garcinone B reduces prostaglandin E2 release and NF-kB-mediated transcription in C6 rat glioma cells2006

    • Author(s)
      Yamakuni, T., Aoki, K., Nakatani, K., Nondo, N., Oku, H., Ishiguro, K. and Ohizumi, Y.
    • Journal Title

      Neuroscience Letters 394

      Pages: 206-210

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Development of an in vivo bioassay method for primary preventive substances of the metabolic syndrome-Effect of Kinginkacha-2006

    • Author(s)
      Oku. H., Ogawa, Y., Iwaoka, E., Ishiguro, K.
    • Journal Title

      Yakugaku Zasshi 126

      Pages: 158-160

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Primary preventive teas on metabolic syndrome-Kinginkacha, Kinkacha and Kudingcha-2006

    • Author(s)
      Oku. H., Ogawa, Y., Iwaoka, E., lshiguro, K.
    • Journal Title

      Yakugaku Zasshi 126

      Pages: 114-115

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Allergy-preventive Phenolic Glycoside from Populus sieboldii2006

    • Author(s)
      Ogawa, Y., Oku, H., Iwaoka, E., Iinuma, M., Ishiguro, K.
    • Journal Title

      J. Nat. Prod. 69

      Pages: 1215-1217

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Garcinone B reduces prostaglandin E2 release and NF-kB-mediated transcription in C6 rat glioma cells.2006

    • Author(s)
      Yamakuni, T., Aoki, K., Nakatani, K., Nondo, N., Oku, H., Ishiguro, K., Ohizumi, Y.
    • Journal Title

      Neuroscience Letters 394

      Pages: 206-210

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary 2006 Annual Research Report
  • [Journal Article] Allergy-preventive Phenolic Glycoside from Populus sieboldii.2006

    • Author(s)
      Ogawa, Y., Oku, H., Iwaoka, E., Iinuma, M, Ishiguro, K.
    • Journal Title

      J.Nat.Prod. 69

      Pages: 1215-1217

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Primary preventive teas on metabolic syndrome- Kinginkacha, kinkacha and Kudingcha2006

    • Author(s)
      Oku, H., Ogawa, Y., Iwaoka, E., Ishiguro, K.
    • Journal Title

      Yakugaku Zasshi 126

      Pages: 114-115

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Development of an in vivo bioassay method for primary preventive substances of the metabolic syndrome- Effect of Kinginkacha.2006

    • Author(s)
      Oku, H., Ogawa, Y., Iwaoka, E., Ishiguro, K.
    • Journal Title

      Yakugaku Zasshi 126

      Pages: 158-160

    • Related Report
      2006 Annual Research Report
  • [Presentation] 抗ストレス物質を目的とするアッセイ法の開発2008

    • Author(s)
      伊藤 愛, 奥 尚枝, 石黒 京子
    • Organizer
      日本薬学会第128年会
    • Place of Presentation
      横浜
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Development of in vivo assay method for anti-stress agent.2008

    • Author(s)
      Ito, A., Oku, H., Ishiguro, K.
    • Organizer
      128th the Pharmaceutical Society of Japan
    • Place of Presentation
      Yokohama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Primary preventive teas on metabolic syndrome-Mechanisms of Kinginkacha,and Kinkacha-2007

    • Author(s)
      Oku H., Ogawa Y., Iwaoka E., Yamaguchi Y., Kunitomo M. and Ishiguro K.
    • Organizer
      第2回食品薬学シンポジウム
    • Place of Presentation
      静岡
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development and Application of an in vivo Bioassay Method for Allergy-Preventive Substances-Allergy preventive Effect of Xanthorrhoea hastilis-2007

    • Author(s)
      Ishiguro, K., Ogawa, Y., Oku, H., Iwaoka, E. and Iinuma M.
    • Organizer
      Pharmaceutical Sciences World Congress
    • Place of Presentation
      Amsterdam
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 病態動物モデルSHR-SPにおけるハナビラタケの血圧上昇抑制および血流量改善作用2007

    • Author(s)
      岩岡 恵実子, 池田 克巳, 久保 正秀, 柴田 勝, 奥 尚枝, 石黒 京子, 高 明
    • Organizer
      日本薬学会第127年会
    • Place of Presentation
      富山
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] キサントレアのアレルギー発症予防効果2007

    • Author(s)
      小川 優子, 奥 尚枝, 岩岡 恵実子, 飯沼 宗和, 石黒 京子
    • Organizer
      日本薬学会第127年会
    • Place of Presentation
      富山
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Primary preventive teas on metabolic syndrome-Mechanisms of Kinginkacha, and Kinkacha-2007

    • Author(s)
      Oku H., Ogawa Y., Iwaoka E., Yamaguchi Y., Kunitomo M., Ishiguro K.
    • Organizer
      The 2th Symposium on Pharmaceutocal Food Science
    • Place of Presentation
      Shizuoka
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development and Application of an in vivo Bioassay Method for Allergy-Preventive Substances-Allergy preventive effect of Xanthorrhoea hastilis-2007

    • Author(s)
      Ishiguro K., Ogawa Y., Oku H., Iwaoka E., Iinuma M.
    • Organizer
      Pharmaceutical Sciences World Congress
    • Place of Presentation
      Amsterdam
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Effects of Hanabiratake on SHR-SP rat.2007

    • Author(s)
      Iwaoka E., Ikeda K., Kubo M., Shibata M., Oku H., Ishiguro K., Koa M.
    • Organizer
      127th The Pharmaceutical Society of Japan
    • Place of Presentation
      Toyama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Allergy preventive effect of Xanthorrhoea hastilis.2007

    • Author(s)
      Ogawa Y., Oku H. lwaoka E., Iinuma M., Ishiguro K.
    • Organizer
      127th The Pharmaceutical Society of Japan
    • Place of Presentation
      Toyama
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development and Application of an in vivo Bioassay Method for Allergy-Priventive Substances-Effect of Xanthorrhoea hastilis2007

    • Author(s)
      Ishiguro, K., Ogawa, Y., Oku, H., Iwaoka, E., Iinuma, M.
    • Organizer
      Pharmaceutical Sciences World Congress
    • Place of Presentation
      Amsterdam
    • Related Report
      2007 Annual Research Report
  • [Presentation] Development of an in vivo bioassay method for primary preventive substances of the metabolic syndrome-Effect of Kinginkacha-2006

    • Author(s)
      Oku, H., Ogawa, Y., Iwaoka, E. and Ishiguro, K.
    • Organizer
      第16回天然薬物の開発と応用シンポジウム
    • Place of Presentation
      札幌
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of an in vivo bioassay method for Primary Preventive Teas on Metabolic Syndrome-Kinginkacha,Kinkacha and Kudingcha-2006

    • Author(s)
      Oku, H., Ogawa, Y., Iwaoka, E. and Ishiguro, K.
    • Organizer
      第1回食品薬学シンポジウム
    • Place of Presentation
      大阪
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of an in vivo Bioassay method for Primary preventive substances of the Metabolic syndrome-2006

    • Author(s)
      Oku, H., Ogawa, Y., Iwaoka, E. and Ishiguro, K.
    • Organizer
      2nd International Symposium on Lifestyle Related Diseases-Perspectives for Primary Prevention and Treatment in Animal Models and Humans to be held
    • Place of Presentation
      Nishinomiya
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Primary Preventive Teas on Metabolic Syndrome-Kinginkacha,Kinkacha and Kudingcha-2006

    • Author(s)
      Ogawa, Y., Oku, H., Iwaoka, E. and Ishiguro, K.
    • Organizer
      2nd International Symposium on Lifestyle Related Diseases-Perspectives for Primary Prevention and Treatment in Animal Models and Humans to be held
    • Place of Presentation
      Nishinomiya
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of an assay method for anti-allergic effect by using HEL-induced blood flow decrease (Part 2)2006

    • Author(s)
      Oku H., Ogawa Y., Iwaoka E., Ueda H., Okamura H., and Ishiguro K.
    • Organizer
      International Conference on Biodiversity and Natural Products
    • Place of Presentation
      Kyoto
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of an in vivo bioassay method for primary preventive substances of the metabolic syndrome-Effect of Kinginkacha-2006

    • Author(s)
      Oku, H., Ogawa, Y., lwaoka, E., Ishiguro, K.
    • Organizer
      16th Symposium on the Development and Application of Naturally Occurring Drug Materials
    • Place of Presentation
      Sapporo
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of an in vivo Bioassay method for Primary preventive teas on Metabolic syndrome-Kinginkacha, Kinkacha and Kudingcha-2006

    • Author(s)
      Oku, H., Ogawa, Y., lwaoka, E., Ishiguro, K.
    • Organizer
      The 1th Symposium on Pharmaceutocal Food Science
    • Place of Presentation
      Osaka
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of an in vivo Bioassay method for Primary preventive substances of the Metabolic syndrome2006

    • Author(s)
      Oku, H., Ogawa, Y., Iwaoka, E., Ishiguro, K.
    • Organizer
      2nd International Symposium on Lifestyle Related Diseases-Perspectives for Primary Prevention and Treatment in Animal Models and Humans to be held
    • Place of Presentation
      Nishimomiya
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Primary Preventive Teas on Metabolic Syndrome-Kinginkacha, Kinkacha and Kudingcha-2006

    • Author(s)
      Ogawa, Y., Oku. H., Iwaoka, E., Ishiguro, K.
    • Organizer
      2nd International Symposium on Lifestyle Related Diseases-Perspectives for Primary Prevention and Treatment in Animal Models and Humans to be held
    • Place of Presentation
      Nishimomiya
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Development of an assay method for anti-allergic effect by using HEL-induced blood flow decrease(Part 2).2006

    • Author(s)
      Oku H., Ogawa Y., Iwaoka E., Ueda H., Okamura H., Ishiguro K.
    • Organizer
      International Conference on Biodiversity and Natural Products
    • Place of Presentation
      Kyoto
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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