Determination of the liquid-liquid phase separation and the zeta potentials of liquid droplets and crystals in the insulin aqueous solutions

• Project/Area Number: 18550020
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Physical chemistry
• Research Institution: Yamaguchi University
• Principal Investigator: WAIZUMI Kenji Yamaguchi University, 教育学部, 教授 (70260677)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥4,220,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥420,000); Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2007: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000); Fiscal Year 2006: ¥2,400,000 (Direct Cost: ¥2,400,000)
• Keywords: タンパク質 / 結晶成長 / インスリン / 相分離 / ゼータ電位

Research Abstract

タンパク質の機能を理解するには、タンパク質分子の精密な3次元構造を知ることが重要である。そのためには良質の単結晶を必要とするが、多くのタンパク質では良質な単結晶が得られず、研究遂行上のボトルネックとなっている。本研究では、タンパク質結晶の良質な単結晶を得るための研究の一貫として、インスリンの結晶形態制御および濃厚な液滴を経由して結晶が形成される現象についての基礎研究を行い、これまで報告されていなかった幾つかの新たな知見を得た

Research Products

• [Journal Article] Effects of pH and Particle Size on Zeta Potential of Insulin Crystals in their Growth Solutions, J.Jpn (2008)
  • Author(s): KENJI WAIZUMI, NAWATA SHUSUKE, KIMURA ATSUSHI
  • Journal Title: Soc. Microgravity Appl Vol.25, No, 3 Pages: 593-597
  • Peer Reviewed
• [Journal Article] インスリン微粒子のゼータ電位 ー結晶形態変化との関連ー (2007)
  • Author(s): 和泉研二、名和田周助
  • Journal Title: 日本結晶成長学会誌 33・4 Pages: 148-148
• [Journal Article] インスリン結晶の核形成に対する微小重力の影響 (2007)
  • Author(s): 和泉研二
  • Journal Title: Space Utilization Research 23 Pages: 9-11
  • NAID: 120006830745
• [Journal Article] インスリン微粒子のゼータ電位 -結晶形態変化との関連- (2007)
  • Author(s): 和泉研二
  • Journal Title: 日本結晶成長学会誌 33・4 Pages: 148-148
• [Presentation] Effects of Zeta Potential on the Morphological change of Insulin Crystals (2008)
  • Author(s): KENJI WAIZUMI
  • Organizer: Japan-Netherlands Symposium on Crystal Growth - Theory and In-situ Measurements-
  • Place of Presentation: Sapporo
  • Year and Date: 2008-10-21
• [Presentation] Effects of Zeta Potential on the Morphological change of Insulin Crystals (2008)
  • Author(s): Kenji Waizumi
  • Organizer: Japan-Netherlands Symposium on Crystal Growth-Theory and In-situ Measurements-
  • Place of Presentation: Sapporo
  • Year and Date: 2008-10-21
• [Presentation] Effects of pH and Particle Size on Zeta Potential of Insulin Crystals in their Growth Solutions (2007)
  • Author(s): KENJI WAIZUMI
  • Organizer: Third International Symposium on Physical Sciences in Space
  • Place of Presentation: NARA
  • Year and Date: 2007-10-23
• [Presentation] Effects of pH and Particle Size on Zata Potential of Insulin Crystals in their Growth Solutions (2007)
  • Author(s): Kenji Waizumi
  • Organizer: Third International Symposium on Physical Sciences in Space
  • Place of Presentation: Nara
  • Year and Date: 2007-10-23
• [Presentation] Relationship between the Crystal Morphology and Zeta Potential of Insulin (2007)
  • Author(s): KENJI WAIZUMI
  • Organizer: 2nd International Topical Team Meeting on Crystal Growth
  • Place of Presentation: NARA
  • Year and Date: 2007-10-21
• [Presentation] インスリン微粒子のゼータ電位 (2007)
  • Author(s): 和泉研二、木村敦
  • Organizer: 日本化学会第87回春季年会
  • Place of Presentation: 関西大学
  • Year and Date: 2007-03-27
• [Presentation] Nucleation and Growth of Insulin Crystals (2006)
  • Author(s): KENJI WAIZUMI
  • Organizer: 19th Geneal Meeting of the International Mineralogical Assosiation
  • Place of Presentation: KOBE
  • Year and Date: 2006-07-27