Development for the method of minute plant cell analysis by fluorescence. correlation spectroscopy.
| Project/Area Number |
18550080
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Analytical chemistry
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| Research Institution | Tokyo University of Pharmacy and Life Science |
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Principal Investigator |
FUJIWARA Kitao Tokyo University of Pharmacy and Life Science, School of Life Sciences, Professor (90090521)
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| Co-Investigator(Kenkyū-buntansha) |
AOKI Motohide Tokyo University of Pharmacy and Life Sciences, School of Life Sciences, Assistant Professor (30418917)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2006: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | Fluorescence spectroscopy / Micro-algae / Chlorophyl / Correlation Spectroscopy / Nano-particle / Particle size determination / プランクトン / 大腸菌 / 自己相関蛍光法 / FCS / 共焦点顕微鏡 / 粒径測定 / タンパク質 / ナノプランクトン / ピコプランクトン / 自己相関法 / 蛍光分光法 / 微粒子 / シリカナノ粒子 / クロレラ / フローセル |
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| Research Abstract |
Fluorescence correlation spectrometry (FCS) is the novel technique which can determine the size of fluorescent particles. The optical parameters such as diameter of pin-hole aperture and laser beam size were inspected in the response of FCS signal against the variation in the diameters of fluorescent particles. In the ordinary system, con-focal optical configuration was adopted. However, it has been proved that the FCS system with the ordinary optical configuration of fluorescence spectroscopy can determine the size lass than 100 nm. The present system with the ordinary optical fluorescence configuration can successfully determine the mico-algae.
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Report
(3 results)
Research Products
(17 results)
