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Development of threshold-energy dissociation mass spectrometry for characterization of biomolecules

Research Project

Project/Area Number 18550085
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Analytical chemistry
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

NAKAMURA Takemichi  The Institute of Physical and Chemical Research, 物質構造解析チーム, 先任研究員 (10360611)

Project Period (FY) 2006 – 2008
Project Status Completed (Fiscal Year 2008)
Budget Amount *help
¥2,750,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥450,000)
Fiscal Year 2008: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2007: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2006: ¥800,000 (Direct Cost: ¥800,000)
Keywords質量分析法 / 閾値エネルギー解離 / 生体分子 / イオントラップ / 解離反応経路 / 選択的断片化 / 衝突冷却 / 有機化合物定性 / 電子捕獲 / 複合型糖ペプチド / 多重極蓄積支援解離 / 振動励起 / 温度計分子 / 反応経路
Research Abstract

研究成果の概要:低分子有機化合物の定性および定量分析法として質量分析法は最も高感度かつ特異性の高い方法の一つであるが,生体分子の多くに対しては電子イオン化法を適用できず,化合物の同定は非常に困難な作業となる.閾値エネルギー解離法に関する本研究の結果,汎用性が高く小型化可能なイオントラップ質量分析計を用いて,本法を生体分子を含む広範な低分子有機化合物の定性・同定法へと展開していくための基盤を構築できた.

Report

(4 results)
  • 2008 Annual Research Report   Final Research Report ( PDF )
  • 2007 Annual Research Report
  • 2006 Annual Research Report
  • Research Products

    (15 results)

All 2009 2008 2007 2006

All Journal Article (8 results) (of which Peer Reviewed: 7 results) Presentation (7 results)

  • [Journal Article] マススペクトルの解析と有機化合物の定性 - 質量分析実務者のための基礎講座2009

    • Author(s)
      中村健道
    • Journal Title

      Journal of the Mass Spectrometry Society of Japan 57(印刷中)

      Pages: 0-0

    • NAID

      10024837806

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] マススペクトルの解析と有機化合物の定性-質量分析実務者のための基礎講座2009

    • Author(s)
      中村健道
    • Journal Title

      Journal of the Mass Spectrometry Society of Japan 57(印刷中)

    • NAID

      10024837806

    • Related Report
      2008 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Factors governing peptide backbone cleavages in electron capture dissociation of triantennary complex-type N-glycosylated peptide2007

    • Author(s)
      Hongo Y, , Sato A, Nakamura T.
    • Journal Title

      Journal of the Mass Spectrometry Society of Japan 55

      Pages: 279-285

    • NAID

      10019743670

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Electron capture dissociation of triantennary complex-type N-glycosylated peptides: a case of suppressed peptide backbone cleavage2007

    • Author(s)
      Hongo Y, Nakamura T, Sato A.
    • Journal Title

      Journal of the Mass Spectrometry Society of Japan 55

      Pages: 77-82

    • NAID

      130000262293

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Electron capture dissociation of triantennary complex-type N-glycosylated peptides: a case of suppressed peptide backbone cleavage.2007

    • Author(s)
      Hongo, Y., et. al.
    • Journal Title

      Journal of the Mass Spectrometry Society of Japan 55

      Pages: 77-82

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Factors governing peptide backbone cleavages in electron capture dissociation of triantennary complex-type N-glycosylated peptide.2007

    • Author(s)
      Hongo, Y., et. al.
    • Journal Title

      Journal of the Mass Spectrometry Society of Japan 55

      Pages: 279-285

    • Related Report
      2007 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phosphatidylglucoside exists as a single molecular species with saturated fatty acyl chains in developing astroglial membranes2006

    • Author(s)
      Nagatsuka Y, Horibata Y, Yamazaki Y, Kinoshita M, Shinoda Y, Hashikawa T, Koshino H, Nakamura T, Hirabayashi Y.
    • Journal Title

      Biochemistry 45

      Pages: 8742-8750

    • Related Report
      2008 Final Research Report
    • Peer Reviewed
  • [Journal Article] Phosphatidylglucoside exists as a single molecular species with saturated fatty acyl chains in developing astroglial membranes.2006

    • Author(s)
      Nagatsuka, Y. et al.
    • Journal Title

      Biochemistry 45(29)(印刷中)

    • Related Report
      2006 Annual Research Report
  • [Presentation] スペクトル解析と定性 - MS とMS/MS を有機化学的視点から考える2008

    • Author(s)
      中村健道
    • Organizer
      第35回BMSコンファレンス(BMS2008)
    • Place of Presentation
      裏磐梯ロイヤルホテル
    • Year and Date
      2008-07-08
    • Related Report
      2008 Final Research Report
  • [Presentation] スペクトル解析と定性-MSとMS/MSを有機化学的視点から考える2008

    • Author(s)
      中村健道
    • Organizer
      第35回BMSコンファレンス(BMS2008)
    • Place of Presentation
      裏磐梯ロイヤルホテル
    • Year and Date
      2008-07-08
    • Related Report
      2008 Annual Research Report
  • [Presentation] Inconvenient Reality: Is the "Hydrogen-shift" in Electron Capture Dissociation of Peptide Backbone Predictable?2007

    • Author(s)
      Takemichi Nakamura
    • Organizer
      55th ASMS Conference on Mass Spectrometry and Allied Topics
    • Place of Presentation
      Indiana Convention Center in Indianapolis
    • Year and Date
      2007-06-04
    • Related Report
      2008 Final Research Report
  • [Presentation] Inconvenient Reality:Is the"Hydrogen-shift"in Electron Capture Dissociation of Peptide Backbone Predictable?2007

    • Author(s)
      Takemichi Nakamura
    • Organizer
      55th ASMS Conference on Mass Spectrometry and Allied Topics
    • Place of Presentation
      Indiana Convention Center in Indianapolis
    • Year and Date
      2007-06-04
    • Related Report
      2007 Annual Research Report
  • [Presentation] ECD スペクトルの解釈について - 有機マススペクトロメトリーの視点から考える2007

    • Author(s)
      中村健道
    • Organizer
      第55回質量分析総合討論会
    • Place of Presentation
      広島国際会議場
    • Year and Date
      2007-05-16
    • Related Report
      2008 Final Research Report
  • [Presentation] ECDスペクトルの解釈について-有機マススペクトロメトリーの視点から考える2007

    • Author(s)
      中村 健道
    • Organizer
      第55回質量分析総合討論会
    • Place of Presentation
      広島国際会議場
    • Year and Date
      2007-05-16
    • Related Report
      2007 Annual Research Report
  • [Presentation] A proposal for extension of Biemann's nomenclature: peptide backbone fragmentation with odd electron species2006

    • Author(s)
      Takemichi Nakamura
    • Organizer
      22nd Asilomar Conference on Mass Spectrometry: Fundamentals of Gas Phase Ion Chemistry
    • Place of Presentation
      Asilomar Conference Center
    • Year and Date
      2006-10-21
    • Related Report
      2008 Final Research Report

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Published: 2006-04-01   Modified: 2016-04-21  

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