| Project/Area Number |
18550148
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Fukuoka Institute of Technology (2007)
University of Tsukuba (2006) |
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Principal Investigator |
MITA Hajime Fukuoka Institute of Technology, Faculty of Engineering, Professor (00282301)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥4,060,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥360,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2006: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | G-quadruplex DNA / function and structure / Redox potential / Axial ligand / heme / dimerization / NMR / thermodynamics / 四重鎮DNA |
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| Research Abstract |
A G-quadruplex DNA is composed of stacked G-quartets, each of which involves the planar association of four guanine bases. The size and planarity of a G-quartet are well-suited for interacting with a porphyrin ring through π-π stacking. The complexation of G-quadruplex DNAs with porphyrin or metal-porphyrin derivatives has been studied to characterize their molecular recognition of each other as well as to, create catalytic DNAs that exhibit hemoprotein functions. We have demonstrated the formation of a stable coordination complex between heme, the iron(III)-protoporphyrin IX complex, and a parallel G-quadruplex DNA assembled from a single repeat sequence of the human telomere, TTAGGG. The heme-DNA complex was found to be remarkably similar, in various spectroscopic and functional properties, to hemoprotein such as myoglobin.
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