| Budget Amount *help |
¥4,120,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥420,000)
Fiscal Year 2007: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2006: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Research Abstract |
This research has aimed to establish experimental platforms using solid-supported lipid bilayer in order to reveal the relation between the assembling structure and function of photosynthetic membrane proteins, light-harvesting complex 2 (LH2) and light-harvesting mm complex (LH1-RC). Recently, the array structure of LH2 and LH1-RC in a bacterial photosynthetic membrane has been revealed by atomic force microscopy (AFM). In the membrane, hexameric LH1-RCs were surrounded by LH2 molecules, forming 'protein nay' structure. We attempted to construct assembly of LH1-RC and LH2 in a supported lipid bilayer membrane.
LH1, reaction center(RC), and LH1-RC complexes were successfully assembled onto an amino-modified ITO electrode. Enhanced photocurrent generation by RC was observed when the RC was assembled with LHl, indicating that light harvesting and energy transfer by LH1 could work on the electrode.
We have established a novel method for incorporation of LH2 into a phew-separated lipid domai
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n in the membrane. In brief, LH2 incorporated into a cationic vesicle cargo (proteoliposome) is selectively arrayed on the fluid domain of the anionic planar membrane via vesicle fusion and subsequent lateral diffusion. The prceess of arraying has been observed by total internal reflection fluorescence (TIRF) microscopy An LH2/LLH1-RC coexistent array in a supported lipid bilayer was successfully constructed by consecutive deposition of vesicle cargos incorporating LH2 and LH1-RC onto an amino-modified coverslip.
LH1, reaction center (RC), and LH1-RC complexes were successfully assembled onto an amino-modified ITO electrode. Enhanced photocurrent generation by RC was observed when the RC was assembled with LH1, indicating that light harvesting and energy transfer by LH1 could work on the electrode.
A planar lipid bilayer tethered by an avidin-immobilized substrate via avidin-biotin interaction was successfully constructed. The architecture provides thin water layer between the planar membrane and the substrate. The water layer allows the LH1-RC possessing large protrudent hydrophilic domain to diffuse laterally within the membrane. As the experimental platform, this architecture makes it possible to investigate dynamic assembling of LH1-RC and LH2 in the planar membrane Less
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