Enzyme chemical study of heme oxygenase and development for machinery of heme catabolism
| Project/Area Number |
18550153
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Chemistry related to living body
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| Research Institution | Kyushu Institute of Technology |
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Principal Investigator |
SAKAMOTO Hiroshi Kyushu Institute of Technology, 大学院・情報工学研究院, 准教授 (70309748)
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| Co-Investigator(Kenkyū-buntansha) |
YASUNAGA Takuo 九州工業大学, 大学院・情報工学研究院, 教授 (60251394)
東元 祐一郎 久留米大学, 医学部, 准教授 (40352124)
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| Co-Investigator(Renkei-kenkyūsha) |
HIGASHIMOTO Yuichiro 久留米大学, 医学部, 准教授 (40352124)
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| Project Period (FY) |
2006 – 2008
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| Project Status |
Completed (Fiscal Year 2008)
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| Budget Amount *help |
¥4,040,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥540,000)
Fiscal Year 2008: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2007: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2006: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | 酵素反応 / 蛋白質 / ヘム / 構造解析 / シグナル伝達 / ヘムオキシゲナーゼ / シトクロムP450還元酵素 / 熱測定 / 電子顕微鏡 |
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| Research Abstract |
ヘムオキシゲナーゼ(HO)はヘム代謝系の律速酵素で、NADPH-シトクロムP450還元酵素から電子の供給を受け、ヘムを3段階からなる酸素依存性の反応によって分解し、ビリベルジン、一酸化炭素および鉄イオンを生成する。ビリベルジンは、ビリベルジン還元酵素によって直ちにビリルビンに還元される。本研究では、HOの触媒作用機序の解明と、ヘム代謝酵素および関連タンパク質の相互作用の探求を総合的に展開し、HOの生理機能を分子レベルで理解するための基盤作りを行った。
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Report
(4 results)
Research Products
(83 results)
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[Journal Article] Crystal structure of rat heme oxygenase-1 in complex with ferrous verdoheme : presence of a hydrogen bond network on the distal side2009
Author(s)
Sato, H., Sugishima, M., Sakamoto, H., Higashimoto, Y., Shimokawa, C., Fukuyama, K., and Noguchi, M.
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Journal Title
Biochem. J. 419
Pages: 339-345
Related Report
Peer Reviewed
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[Journal Article] Mass spectrometric identifica-tion of lysine residues of heme oxygenase-1 that are involved in its interaction with NADPH-cytochrome P450 reductase.2008
Author(s)
Higashimoto, Y., Sugishima, M., Sato, H., Sakamoto, H., Fukuyama, K., Palmer, G., and Noguchi, M.
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Journal Title
Biochem. Biophys. Res. Commun 367
Pages: 852-858
Related Report
Peer Reviewed
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[Journal Article] Partially functional outer arm dynein in a novel Chlamydomonas mutant expressing a truncated γ heavy chain.2008
Author(s)
Liu, Z., Takazaki, H., Nakazawa, Y., Sakato, M., Yagi, T., Yasunaga, T., King, S.M., and Kamiya, R.
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Journal Title
Eukaryotic Cell 7
Pages: 1136-1145
NAID
Related Report
Peer Reviewed
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[Journal Article] Structural basis for tropomyosin overlap in thin(actin)filaments and the generation of a molecular swivel by troponin-T2008
Author(s)
Murakami, K., Stewart, M., Nozawa, K., Tomii, K., Kudou, N., Igarashi, N., Shirakihara, Y., Wakatsuki, S., Yasunaga, T., and Wakabayashi, T.
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Journal Title
Proc. Nat. Acad. Sci. USA 105
Pages: 7200-7205
NAID
Related Report
Peer Reviewed
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[Journal Article] Electrochemical reduction of ferrous α-verdoheme in complex with heme oxygenase-12007
Author(s)
Sato, H., Higashimoto, Y., Sakamoto, H., Sugishima, M., Takahashi, K., Palmer, G., and Noguchi, M.
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Journal Title
J. Inorg. Biochem 101
Pages: 1394-1399
Related Report
Peer Reviewed
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[Journal Article] Alternative cyanide-binding modes to the haem iron in haem oxygenase2007
Author(s)
Sugishima, M., Oda, K., Ogura, T., Sakamoto, H., Noguchi, M., and Fukuyama, K.
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Journal Title
Acta. Crystallogr. Sect. F Struct. Biol. Cryst. Commun 63
Pages: 471-474
NAID
Related Report
Peer Reviewed
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[Journal Article] X-ray crystallographic and biochemical characterization of the inhibitory action of an imidazole-dioxolane compound on heme oxygenase2007
Author(s)
Sugishima, M., Higashimoto, Y., Oishi, T., Takahashi, K., Sakamoto, H., Noguchi, M., and Fukuyama, K.
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Journal Title
Biochemistry 46
Pages: 1860-1867
Related Report
Peer Reviewed
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[Journal Article] Structural basis for calcium-regulated relaxation of striated muscles at interaction sites of troponin with actin and tropomyosin2007
Author(s)
Murakami, K., Yumoto, F., Ohki, S. Y., Yasunaga, T., Tanokura, M., and Wakabayashi, T.
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Journal Title
Adv. Exp. Med. Biol 592
Pages: 71-86
Related Report
Peer Reviewed
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[Journal Article] An Actin Homolog of the Archaeon Thermoplasma acidophilum That Retains the Ancient Characteristics of Eukaryotic Actin2007
Author(s)
Hara, F., Yamashiro, K., Nemoto, N., Ohta, Y., Yokobori, S., Yasunaga, T., Hisanaga, S., and Yamagishi, A.
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Journal Title
J. Bacteriol 189
Pages: 2039-2045
Related Report
Peer Reviewed
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[Journal Article] Domains 16 and 17 of tropoelastin in elastic fibre formation2007
Author(s)
Wachi, H., Sato, F., Nakazawa, J., Nonaka, R., Szabo, Z., Urban, Z., Yasunaga, T., Maeda, I., Okamoto, K., Starcher B. C., and Li, D. Y
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Journal Title
Biochem. J. 402
Pages: 63-70
Related Report
Peer Reviewed
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[Journal Article] The reactions of heme- and verdoheme-heme oxygenase-1 complexes with FMN-depleted NADPH-cytochrome P450 reductase-electrons required for verdoheme oxidation can be transferred through a pathway not involving FMN.2006
Author(s)
Higashimoto, Y., Sato, H., Sakamoto, H., Takahashi, K., Palmer, G., and Noguchi, M.
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Journal Title
J. Biol. Chem 281
Pages: 31659-31667
NAID
Related Report
Peer Reviewed
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[Journal Article] Atomic model construction of protein complexes from electron micrographs and visualization of their 3D structure using VR system.2006
Author(s)
Noda, K., Nakamura, M., Nishida, R., Yoneda, Y., Yamaguchi, Y., Tamura, Y., Nakuamura, H., and Yasunaga, T.
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Journal Title
J. Plasma Physics 72
Pages: 1037-1040
Related Report
Peer Reviewed
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