Development of polycation-immobilized cellulose particles for selective DNA adsorption based its multipoint attachment
| Project/Area Number |
18550198
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Polymer/Textile materials
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| Research Institution | Kumamoto University |
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Principal Investigator |
SAKATA Masayo Kumamoto University, Graduate school of Science & Technology, Assistant Professor (60187391)
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| Co-Investigator(Kenkyū-buntansha) |
SASAKI Mitsuru Kumamoto University, Graduate school of Science & Technology, Associate Professor (40363519)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥4,190,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥390,000)
Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2006: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | DNA adsorption / polvcation / dimethvlaminopropylacrylamide / ligand / polvethvleneimine / chromatographic separation / pore size / ionic strength / セルロース粒子 / イオン相互作用 / イオン強度 |
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| Research Abstract |
We have focused a method for the selective removal of DNA from various cellular products using columns packed with polycation-immobilized cellulose beads. Polyethyleneimine (PEI), poly-N,N-dimethylaminopropylacrylamide (poly (DAPA)) and poly (ε-lysine) (PεL), all of which have cationic properties, were used as the ligands on the beads. Cellufine-GC15[○!R] and -CPC^[○!R] were used as cellulose matrices. Adsorption of DNA by the beads was determined using a batchwise method or a column method. Each bead type showed high DNA-adsorbing activity at pH 7.0 and ionic strengths of μ= 0.05-0.8. The larger the pore size of the beads, the larger the DNA-adsorbing activity. The DNA- adsorbing capacities per wet-mL of PEI-, poly(DAPA)- and PεL-immobilized Cellufine-CPC with large pore sizes, were 3.7, 3.2 and 1.8 mg, respectively. When a protein, such as bovine serum albumin (BSA) or γ-globulin, was present in solution with the DNA under physiological conditions (pH 7.0,μ= 0.2), the DNA selectivity of the PEI-immobilized Cellufine-CPC columns was unsatisfactory, because both the DNA and the protein were adsorbed onto the column. In contrast, the poly (DAPA) -immobilized Cellufine-CPC column selectively removed DNA from each protein solution contaminated with DNA under similar conditions : the DNA concentration in each treated protein solution was below 10 ng /mL, and high recovery of each protein (>92%) was obtained.
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Report
(3 results)
Research Products
(17 results)
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[Journal Article] Selective removal of DNA from bioproducts by polycationimmobilized cellulose beads2006
Author(s)
M., Sakata, M., Nakayama, K., Yanagi, M., Sasaki, M., Kunitake, C., Hirayama
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Journal Title
Journal of Liquid Chromatography & Related Technologies 29
Pages: 2499-2512
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Chromatographic separation of DNA from bio-product using polycation- immobilized polymer beads2007
Author(s)
M., Sakata, M., Nakayama, R., Nakayama, S., Sakamoto, M., Kunitake
Organizer
31th International Symposium & Exhibit on High Performance Liquid Phase Separations and Related Techniques
Place of Presentation
Ghent, Belgium
Year and Date
2007-06-19
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Novel preparation technique of mono-dispersed polystyrene particles based on silica particled dispersed aqueous without stirring and hydrophilic initiator2007
Author(s)
S., Kawano, S., Nishi, R., Umeza, M., Sakata, M., Kunitake
Organizer
5th International Forum of New Waves in Supramolecular Chemistry and Superstructured Materials, solution
Place of Presentation
Kumamoto, Japan
Year and Date
2007-01-22
Description
「研究成果報告書概要(欧文)」より
Related Report
