Development of new amperometric biosensors based on organic-inorganic hybrid ultra-thin films

• Project/Area Number: 18560005
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied materials science/Crystal engineering
• Research Institution: Tokyo University of Marine Science and Technology
• Principal Investigator: OHNUKI Hitoshi Tokyo University of Marine Science and Technology, 海洋工学部, 助教 (60223898)
• Co-Investigator(Kenkyū-buntansha): IZUMI Mitsuru 東京海洋大学, 海洋工学部, 教授 (50159802)
• Project Period (FY): 2006 – 2008
• Project Status: Completed (Fiscal Year 2008)
• Budget Amount: ¥3,840,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥540,000); Fiscal Year 2008: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000); Fiscal Year 2007: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2006: ¥1,500,000 (Direct Cost: ¥1,500,000)
• Keywords: 有機-無機ハイブリッド / バイオセンサー / ラングミュア・ブロジェット膜 / バイオセンサ / プルシアンブルー / コレステロールオキシターゼ / コレステロールセンサ / 超薄膜 / グルコースオキシターゼ / グルコース / 酵素 / アンペロメトリー

Research Abstract

正に帯電するカチオン性長鎖アルキル分子を鉄シアノ塩であるプルシアンブルー水溶液上に展開し、その水面上単分子膜を基板上に累積するとナノサイズのプルシアンブルークラスターを含む超薄膜が得られる.この有機-無機ハイブリッド超薄膜は高精度なバイオセンサの構成材料として非常に有望であることが分かった. すなわち、様々な酸化酵素を薄膜内に導入することにより、非常に低い印加電圧で動作する各種バイオセンサを作り出すことができる.

Research Products

• [Journal Article] Immobilization of glucose oxidase in Langmuir-Blodgett films containing Prussian blue nano-clusters (2008)
  • Author(s): Hitoshi Ohnuki, Takafumi Saiki, Akira Kusakari, Masaki Ichihara, Mitsuru Izumi
  • Journal Title: Thin Solid Films vol.516 Pages: 8860-8864
  • Peer Reviewed
• [Journal Article] Preparation of an enzymatic glucose sensor based on hybrid organic-inorganic Langmuir-Blodgett films : Adsorption of glucose oxidase into positively charged molecular layers (2008)
  • Author(s): Akira Kusakari, Mitrusu Izumi and Hitoshi Ohnuki
  • Journal Title: Colloids and Surfaces A : Physicochemical and Engineering Aspects vol.321 Pages: 47-51
  • Peer Reviewed
• [Journal Article] Immobilization of glucose oxidase in Langmuir-Blodgett films containing Prussian blue nano-clusters. (2008)
  • Author(s): H. Ohnuki, T. Saiki, A. Kusaka ri, M. Ichihara, M. Izumi
  • Journal Title: Thin Solid Films 516 Pages: 8860-8864
  • Peer Reviewed
• [Journal Article] Preparation of an Enzymatic Glucose Sensor based on Hybrid Inorganic-Organic Langmuir-Blodgett Films: Adsorption of Glucose Oxidase into Positively-Charged Molecular Layers (2008)
  • Author(s): A. Kusakari, M. Izumi, and H. Ohnuki
  • Journal Title: Colloids and Surfaces A: Physicochemical and Engineering (印刷中)
  • Peer Reviewed
• [Journal Article] Incorporation of Glucose Oxidase into Langmuir-Blodgett Films Based on Prussian Blue Applied to Amperometric Glucose Biosensor (2007)
  • Author(s): Hitoshi Ohnuki, Takafumi Saiki, Akira Kusakari, Hideaki Endo, Masaki Ichihara and Mitsuru Izumi
  • Journal Title: Langmuir vol.23 Pages: 4675-4681
  • Peer Reviewed
• [Journal Article] Incorporation of Glucose Oxidase into Langmuir-Blodgett Films Based on Prussian Blue Applied to Amperometric Glucose Biosensor (2007)
  • Author(s): Hitoshi OHNUKI, Takafumi Saiki, Akira Kusakari, Hideaki Endo, Masaki Ichihara, Mitsuru Izumi
  • Journal Title: Langmuir 23 Pages: 4675-4681
• [Journal Article] Metal ion adsorption to conducting organic langmuir-blodgett films (2006)
  • Author(s): Nobuaki Watanabe, Hitoshi Ohnuki, Mitsuru Izumi and Tatsuro Iamakubo
  • Journal Title: Colloids and Surfaces A : Physicochem. Eng. Aspects vol.284-285 Pages: 640-642
  • Peer Reviewed
• [Journal Article] New Molecular combinations for spontaneous mixed valence state formation between EDO-TTF derivatives and carboxylic acids (2006)
  • Author(s): Hitoshi Ohnuki, Takafumi Saiki, Tatsuro Imakubo and Mitsuru Izumi
  • Journal Title: Colloids and Surfaces A : Physicochem. Eng. Aspects vol.284-285 Pages: 15-19
  • Peer Reviewed
• [Presentation] Fabrication of enzymatic biosensors based on hybrid organic-inorganic LB film (2009)
  • Author(s): H. Ohnuki, R. Honjo, H. Endo and T. Imakubo, M. Izumi
  • Organizer: First International Conference on Multifunctional, Hybrid and Nanomaterial
  • Place of Presentation: Tours(France)
  • Year and Date: 2009-03-19
• [Presentation] Fabrication of enzymatic biosensors based on hybrid organic-inorganic LB films (2009)
  • Author(s): H. Ohnuki, R. Honjo, H. Endo, T. Imakubo, M. Izumi
  • Organizer: First International Conference on Multifunctional, Hybrid and Nanomaterials
  • Place of Presentation: Vinci Convention Centre, Tours, France
  • Year and Date: 2009-03-19
• [Presentation] Amperometric Cholesterol Biosensors Based on Hybrid Inorganic-Organic LB films (2008)
  • Author(s): Hitoshi Ohnuki, Rikimaru Honjo, Mitsuru Izumi, Hideaki Endo and Tatsuro Imakubo
  • Organizer: 8th International Conference on Nano-Molecular Electronics
  • Place of Presentation: Kobe(Japan)
  • Year and Date: 2008-12-18
• [Presentation] Amperometric Cholesterol Biosensors Based on Hybrid Inorganic-Organic LB films (2008)
  • Author(s): H. Ohnuki, R. Honjo, M. Izumi, H. Endo, T. Imakubo
  • Organizer: 8th International Conference on Nano-Molecular Electronics
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2008-12-17
• [Presentation] 有機-無機複合構造による超薄膜コレステロールセンサの開発 (2008)
  • Author(s): 大貫等、本上力丸、今久保達郎、遠藤英明、和泉充
  • Organizer: 第69回応用物理学会学術講演会
  • Place of Presentation: 中部大学
  • Year and Date: 2008-09-05
• [Presentation] 有機-無機複合構造による超薄膜コレステロールセンサの開発 (2008)
  • Author(s): 大貫 等, 本上力丸, 今久保達郎, 遠藤英明, 和泉 充
  • Organizer: 応用物理学会
  • Place of Presentation: 中部大学春日井キャンパス
  • Year and Date: 2008-09-03
• [Presentation] 有機-無機複合構造による新しい酵素型グルコースセンサの開発(III) (2008)
  • Author(s): 草刈顕、大貫等、和泉充
  • Organizer: 第55回応用物理学関係連合講演会
  • Place of Presentation: 日本大学
  • Year and Date: 2008-03-30
• [Presentation] 有機-無機複合構造による新しい酵素型グルコースセンサの開発III (2008)
  • Author(s): 草刈 顕、大貫 等、和泉 充
  • Organizer: 応用物理学会
  • Place of Presentation: 日本大学理工学部船橋キャンパス
  • Year and Date: 2008-03-30
• [Presentation] 有機-無機ハイブリッド系ラングミュア・ブロジェット膜を用いたグルコースセンサの開発-カチオン性超薄膜へのグルコース酸化酵素の吸着特性- (2007)
  • Author(s): 大貫 等、草刈 顕、和泉 充
  • Organizer: 電気学会 誘電・絶縁材料研究会
  • Place of Presentation: 新潟大学
  • Year and Date: 2007-11-09
• [Presentation] Preparation of a New Enzymatic Glucose Sensor based on Hybrid Inorganic-Organic LB films (2007)
  • Author(s): Akira Kusakari, Hitoshi Ohnuki and Mitsuru Izumi
  • Organizer: The 12th International Conference on Organized Molecular Films
  • Place of Presentation: Krakow(Poland)
  • Year and Date: 2007-07-03
• [Presentation] Preparation of a New Enzymatic Glucose Sensor based on Hybrid Inorganic-Organic LB films (2007)
  • Author(s): Akira Kusakari, Hitoshi Ohnuki, and Mitsuru Izumi
  • Organizer: The 12th International Conference on Organized Molecular Films
  • Place of Presentation: Krakow(ポーランド)
  • Year and Date: 2007-07-02
• [Presentation] プルシアンブルー系有機-無機複合LB膜のグルコースセンサ特性 (2007)
  • Author(s): 草刈顕、大貫等、和泉充
  • Organizer: 電気化学会第74 回大会
  • Place of Presentation: 東京理科大学
  • Year and Date: 2007-03-29
• [Presentation] 有機-無機複合構造による新しい酵素型グルコースセンサの開発(II) (2007)
  • Author(s): 草刈顕、大貫等、和泉充
  • Organizer: 第54回応用物理学関係連合講演会
  • Place of Presentation: 青山学院大学
  • Year and Date: 2007-03-29
• [Presentation] 有機-無機複合Langmuir-Blodgett膜のバイオセンサ応用 (2007)
  • Author(s): 大貫等、草刈顕、遠藤英明、才木隆史、和泉充
  • Organizer: 平成19年度電気学会全国大会
  • Place of Presentation: 富山大学
  • Year and Date: 2007-03-17
• [Presentation] Immobilization of glucose oxidase in Langmuir-Blodgett films based on Prussian Blue (2006)
  • Author(s): Hitoshi Ohnuki, Takafumi Saiki, Akira Kusakari and Mitsuru Izumi
  • Organizer: 10th European Conference on Organised Film
  • Place of Presentation: Riga(Latvia)
  • Year and Date: 2006-08-23
• [Remarks]
  • URL: http://www.kaiyodai.ac.jp/Japanese/db/0020/0520/BA_40344036.html
• [Remarks] 東京海洋大学研究者情報 個人ページ
  • URL: http://www.kaiyodai.ac.jp/Japanese/db/0020/0520/TH_40344036.html
• [Patent(Industrial Property Rights)] 生体高分子およびナノ構造体への電気的な接触を確立する技術 (2008)
  • Inventor(s): 大貫等、和泉充、草刈顕
  • Industrial Property Number: 2008-035432
  • Filing Date: 2008-02-22
• [Patent(Industrial Property Rights)] 生体高分子およびナノ構造体への電気的な接触を確立する技術 (2008)
  • Inventor(s): 大貫 等
  • Industrial Property Rights Holder: 大貫 等
  • Filing Date: 2008-02-18
• [Patent(Industrial Property Rights)] バイオセンサとその製造方法
  • Inventor(s): 大貫 等, 和泉 充, 才木隆史
  • Industrial Property Rights Holder: 東京海洋大学