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Ecotoxicity evaluation of river water and sewage effluent using aquatic organisms and investigation of identification method for its causative toxic chemicals

Research Project

Project/Area Number 18560531
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Civil and environmental engineering
Research InstitutionKyoto University

Principal Investigator

YAMASHITA Naoyuki  Kyoto University, Faculty of Engineering, Lecturer (90391614)

Co-Investigator(Kenkyū-buntansha) TANAKA Hiroaki  Kyoto University, Faculty of Engineering, Professor (70344017)
Project Period (FY) 2006 – 2007
Project Status Completed (Fiscal Year 2007)
Budget Amount *help
¥4,010,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥510,000)
Fiscal Year 2007: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2006: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordsexotoxicity / sewage effluent / river water / water pollution / chemical substance / 土木環境システム
Research Abstract

Many kinds of chemical substances have been developed with the advancement of science and technology. The chemical substances with low level concentrations released into the aquatic environment would make potential environmental pollution on the aquatic organisms. Therefore, it is important to evaluate the impact by waste effluents containing the chemicals and identify causative chemicals which show toxicity on the aquatic organisms.
In this research, acquisition of ecotoxicological data for chemical substances released into the aquatic environment and establishment of ecotoxicological experimental system using the solid phase extraction technique were conducted. Ecotoxicity of actual environmental samples such as river water and sewage effluent was evaluated and it was tried to identify the causative chemicals present in environmental water samples.
From the results of ecotoxicological tests for pharmaceuticals, which are recently focused as potential environmental pollutants in the aquatic environment, antibiotics showed higher toxicity to aquatic organisms compared with that for other types of pharmaceuticals. Ecotoxicological experimental system combined with the solid phase extraction technique was established and applied to the water samples taken from Lake Biwa and Yodo River basin. High toxicity was shown for the samples of sewage effluents and river water downstream from the sewage effluent discharge. Identification of the causative chemical responsible for toxicity on aquatic organisms contained in water samples taken from Yodo River basin was conducted. Combination of fractionating using HPLC and bioassay was proved to be useful tool for identifying causative chemicals and the substance of about 600 of molecular weight is suggested to be one of causative chemicals for the ecotoxicity.

Report

(3 results)
  • 2007 Annual Research Report   Final Research Report Summary
  • 2006 Annual Research Report
  • Research Products

    (29 results)

All 2008 2007 2006

All Journal Article (9 results) (of which Peer Reviewed: 3 results) Presentation (20 results)

  • [Journal Article] 淀川流域の下水処理場放流水と支川における医薬品の存在実態2007

    • Author(s)
      杉下寛樹, 山下尚之, 田中宏明, 田中周平, 藤井滋穂, 宝輪勲, 小西千絵
    • Journal Title

      環境工学研究論文集 44

      Pages: 307-312

    • NAID

      130003775898

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Occurrence of Pharmaceuticals in sewage effluent and tributary inYodo River basin2007

    • Author(s)
      H. Sugishita, N. Yamashita, H. Tanaka, S. Tanaka, S. Fujii, I. Howa, C. Konishi
    • Journal Title

      Environmental Engineering Research Vol. 44

      Pages: 307-312

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 藻類生長阻害試験を用いた医薬品の生態リスク評価2007

    • Author(s)
      福永彩, 杉下寛樹, 山下尚之, 田中宏明
    • Journal Title

      第41回日本水環境学会年会講演集 41

      Pages: 200-200

    • Related Report
      2006 Annual Research Report
  • [Journal Article] 藻類生長阻害試験を用いた医薬品の毒性評価2006

    • Author(s)
      福永彩, 山下尚之, 田中宏明
    • Journal Title

      環境工学研究論文集 43

      Pages: 57-63

    • NAID

      130003949498

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary 2006 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Effects of antibacterial agents, levofloxacin and clarithromycin, on aquatic organisms2006

    • Author(s)
      N. Yamashita, M. Yasojima, N. Nakada, K. Miyajima, K. Komori
    • Journal Title

      Water Science and Technology 53

      Pages: 65-72

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
    • Peer Reviewed
  • [Journal Article] Toxicity evaluation of Pharmaceuticals using algal growth inhibition test2006

    • Author(s)
      A. Fukunaga, R. Nagao, N. Yamashita, H. Tanaka
    • Journal Title

      Environmental Engineering Research Vol. 43

      Pages: 57-63

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] Effects of antibacterial agents, levofloxacin and clarithromycin, on aquatic organisms2006

    • Author(s)
      N. Yamashita, M. Yasojima, N. Nakada, K. Miyajima, K. Komori
    • Journal Title

      Water Science and Technology Vol. 53

      Pages: 65-72

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Journal Article] 藻類生長阻害試験を用いた人用および動物用医薬品の生態毒性評価2006

    • Author(s)
      福永彩, 山下尚之, 田中宏明
    • Journal Title

      土木学会第60回年次学術講演会講演集 60

      Pages: 7-30

    • Related Report
      2006 Annual Research Report
  • [Journal Article] Effects of antibacterial agents, levofloxacin and clarithromycin, on aquatic organisms2006

    • Author(s)
      Yamashita, N., Yasojima, M., Nakada, N., Miyajima, K., Komori, K., Suzuki, Y., Tanaka, H.
    • Journal Title

      Water Science and Technology 53

      Pages: 65-72

    • Related Report
      2006 Annual Research Report
  • [Presentation] 発光性細菌を用いた毒性試験に基づく水環境中PPCPsの初期リスク評価2008

    • Author(s)
      杉下寛樹, 山下尚之, 田中宏明
    • Organizer
      第42回日本水環境学会年会
    • Place of Presentation
      名古屋
    • Year and Date
      2008-03-20
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Primary ecological risk evaluation of PPCPs in water environment based on toxicity test using luminescent bacteria2008

    • Author(s)
      H. Sugishita, N. Yamashita, H. Tanaka
    • Organizer
      42th annual conference oh water environment
    • Place of Presentation
      Nagoya, Japan
    • Year and Date
      2008-03-20
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Prioritization of the Pharmaceuticals Detected in River Water in Japan Based on Algal Growth Inhibition Test2008

    • Author(s)
      A. Fukunaga, N. Yamashita, H. Tanaka
    • Organizer
      Pharmaceutical Products in the Environment : Trends Toward Lowering Occurrence and Impact
    • Place of Presentation
      Nimes, France
    • Year and Date
      2008-02-20
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Prioritization of the pharmaceuticals detected in river water in Japan based on algal growth inhibition test2008

    • Author(s)
      A. Fukunaga, N. Yamashita, H. Tanaka
    • Organizer
      Pharmaceutical Products in the Environment : Trends Tbward Lowering Occurrence and Impact
    • Place of Presentation
      Nimes, France
    • Year and Date
      2008-02-20
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Prioritization of the Pharmaceuticals Detected in River Water in Japan Based on Algal Growth Inhibition Test2008

    • Author(s)
      A. Fukunaga, N. Yamashita and H. Tanaka
    • Organizer
      Pharmaceutical Products in the Environment: Trends Toward Lowering Occurrence and Impact
    • Place of Presentation
      Nimes, France
    • Year and Date
      2008-02-20
    • Related Report
      2007 Annual Research Report
  • [Presentation] PPCPs in Yodo River System : Rough Classification Based on the Occurrences2007

    • Author(s)
      H. Sugishita, R. Nagao, N. Yamashita. H. Tanaka, I. Howa, C. Konishi
    • Organizer
      2nd International workshop on rainwter and reclaimed water for urban sustainable water use
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2007-10-04
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] An ecological risk assessment approach for Pharmaceuticals in effluent from wastewater treatment plants2007

    • Author(s)
      A. Fukunaga, R. Nagao, N. Yamashita, H. Tanaka
    • Organizer
      2nd International workshop on rainwter and reclaimed water for urban sustainable water use
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2007-10-04
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] An ecological risk assessment approach for pharmaceuticals in effluent from wastewater treatment plants2007

    • Author(s)
      A. Fukunaga, R. Nagao, N. Yamashita, H. Tanaka
    • Organizer
      2nd International workshop on rainwter and reclaimed water for urban sustainable water use
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2007-10-04
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] PPCPs in Yodo River System: Rough Classification Based on the Occurrences2007

    • Author(s)
      H. Sugishita, R. Nagao, N. Yamashita, H. Tanaka, I. Howa and C. Konishi
    • Organizer
      2nd International workshop on rainwter and reclaimed water for urban sustainable water use
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2007-10-04
    • Related Report
      2007 Annual Research Report
  • [Presentation] 淀川水系における96物質の医薬品の存在実態調査2007

    • Author(s)
      杉下寛樹, 田中宏明, 宝輪勲
    • Organizer
      土木学会第62回年次学術講演会
    • Place of Presentation
      広島
    • Year and Date
      2007-09-12
    • Related Report
      2007 Annual Research Report
  • [Presentation] 水生生物に対する医薬品類の毒性影響2007

    • Author(s)
      福永彩, 杉下寛樹, 山下尚之, 田中宏明
    • Organizer
      第29回京都大学環境衛生工学研究会シンポジウム
    • Place of Presentation
      京都
    • Year and Date
      2007-07-19
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] 淀川水系における医薬品の存在実態調査2007

    • Author(s)
      杉下寛樹, 長尾亮治, 山下尚之, 田中宏明, 宝輪勲, 小西千絵
    • Organizer
      第29回京都大学環境衛生工学研究会シンポジウム
    • Place of Presentation
      京都
    • Year and Date
      2007-07-19
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] Toxicity effects of Pharmaceuticals on aquatic organisms2007

    • Author(s)
      A. Fukunaga, H. Sugishita, N. Yamashita, H. Tanaka
    • Organizer
      29th annual symposium on environmental and sanitary enginnering of Kyoto University
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2007-07-19
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Occurrence of pharmaceuticals in Yodo River basin2007

    • Author(s)
      H. Sugishita, R. Nagao, N. Yamashita, H. Tanaka, I. Houwa, C. Konishi
    • Organizer
      29th annual symposium on environmental and sanitary enginnering of Kyoto University
    • Place of Presentation
      Kyoto, Japan
    • Year and Date
      2007-07-19
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] A risk assessment approach using reliability analysis for pharmaceuticals in discharge from sewage treatment plants2007

    • Author(s)
      A. Fukunaga, R. Nagao, N. Yamashita, H. Tanaka
    • Organizer
      5th IWA Specialised Conference on Assessment and Control of Micropollutants/Hazardous Substances in Water
    • Place of Presentation
      Frankfurt, Germany
    • Year and Date
      2007-06-18
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Annual Research Report 2007 Final Research Report Summary
  • [Presentation] A risk assessment approach using reliability analysis for pharmaceuticals in discharge from sewage treatment plants2007

    • Author(s)
      A. Fukunaga, R. Nagao, N. Yamashita, H. Tanaka
    • Organizer
      5th IWA Specialised Conference on Assessment and Control of Micropollutants /Hazardous Substances in Water
    • Place of Presentation
      Frankfurt, Germany
    • Year and Date
      2007-06-18
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 藻類生長阻害試験を用いた医薬品の生態リスク評価2007

    • Author(s)
      福永彩, 杉下寛樹, 山下尚之, 田中宏明
    • Organizer
      第41回日本水環境学会年会講演集
    • Place of Presentation
      大阪
    • Year and Date
      2007-03-15
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Ecological risk evaluation of Pharmaceuticals by algal growth inhibition test2007

    • Author(s)
      A. Fukunaga, H. Sugishita, N. Yamashita, H. Tanaka
    • Organizer
      41th annual conference on water environment
    • Place of Presentation
      Osaka, Japan
    • Year and Date
      2007-03-15
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] 藻類生長阻害試験を用いた人用および動物用医薬品の生態毒性評価2006

    • Author(s)
      福永彩, 山下尚之, 田中宏明
    • Organizer
      土木学会第61回年次学術講演会講演集
    • Place of Presentation
      草津
    • Year and Date
      2006-09-20
    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2007 Final Research Report Summary
  • [Presentation] Ecotoxicity evaluation of Pharmaceuticals for human and veterinary use by algal growth inhibition test2006

    • Author(s)
      A. Fukunaga, N. Yamashita, H. Tanaka
    • Organizer
      61th annual conference of civil engineering
    • Place of Presentation
      Kusatsu, Japan
    • Year and Date
      2006-09-20
    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2007 Final Research Report Summary

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Published: 2006-04-01   Modified: 2016-04-21  

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