Development of recognition device for biomolecules using polymer gel-QCM conjugate
| Project/Area Number |
18560729
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Properties in chemical engineering process/Transfer operation/Unit operation
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| Research Institution | Tokyo Institute of Technology (2007)
Institute of Research and Innovation (2006) |
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Principal Investigator |
SEIDA Yoshimi Tokyo Institute of Technology, Interdisciplinary Graduate School of Science and Technology, Guest Associate Professor (60216504)
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| Co-Investigator(Kenkyū-buntansha) |
佐野 景子 (瀧 景子) (財)産業創造研究所, 生物工学部, 研究員 (50332284)
岩佐 敏 (財)産業創造研究所, 化学研究部, 研究員 (70425768)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥300,000)
Fiscal Year 2007: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2006: ¥2,300,000 (Direct Cost: ¥2,300,000)
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| Keywords | Polymer Gel / Biomolecule / Molecular Recognition / Sensor / Antigen-Antibody Reaction / Quartz Crystal Microbalance / Vicso-elasticity / Stimuli-responsiveness / デバイス |
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| Research Abstract |
First, method to synthesized micro size hydrogels on the surface of quartz crystal microbalance, QCM, was developed using N-isopropylacrylamide and its copolymer. The gel physically attached on the surface of QCM worked well as a visco-elastic body under the vibration of the quartz. The gold electrode was preferable for the physical attachment of microgel on the QCM. The method to introduce bovine serum albumin was developed. The protein was introduced into the polymer network of gel chemically through covalent bonding. The phase behavior and QCM response of the microgel were investigated as a function of solution pH, ionic strength and solution temperature. The QCM with 5MHz basic resonation responded with high sensitivity to the environmental change quickly. The change in resonation frequency and viscosity term of the QCM appeared due to the visco-elasitic property change of the gel in the environment. The gel contact with very small amount of organic molecules responded for a wide variety of concentration of the organic molecules. When a anti-body of introduced proteins interacted with the protein, the visco-elasticity of the gel responded sensitively with change in resonation of the QCM. The results obtained in the present study indicated that the micro gel coupled with the QCM was potential device for molecular sensing with high sensitivity.
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Report
(3 results)
Research Products
(44 results)
