Genetic and proteomic analysis of novel antimicrobial proteins from rice and their application to harmful microorganisms
| Project/Area Number |
18560749
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Niigata University |
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Principal Investigator |
TANIGUCHI Masayuki Niigata University, Institute of Science and Technology, Professor (00163634)
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| Project Period (FY) |
2006 – 2007
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| Project Status |
Completed (Fiscal Year 2007)
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| Budget Amount *help |
¥3,980,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥480,000)
Fiscal Year 2007: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2006: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Antimicrobial protein / Rice protein / Antimicrobial activity / Protein purification / Periodontal disease / システインプロテアーゼ / 歯周病菌 / プロテアーゼ阻害剤 |
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| Research Abstract |
In this study, the preparation of cysteine protease inhibitors (oryzacystatins: Ocs) as a recombinant protein and their properties were investigated. The screening of antimicrobial proteins from rice and rice bran against Porphyromonas gingivalis was also studied. The results obtained in this study were summarized as follows.
1. Five types of recombinant-OCs were expressed in Escherichia coli BL21 as the GST or His-tag fusion protein and purified by the conventional method. The inhibitory activities of OCs for several cysteine proteases were estimated.
2. To measure sensitively antimicrobial activity, turbidity assay, ATP-chemical luminescence assay and fluorescence assay using DNA-binding reagent were developed and their performances were compared.
3. The proteins inhibitory to proteases (Arg- and Lys-gingipains) from P. gingivalis were detected in the extracts of rice and rice bran.
4. The proteins of MW 1-16 kDa obtained from the extract of rice bran have antimicrobial activity against P. gingivalis. The chemical structure of the proteins purified by two-dimensional electrohoresis was analyzed by MALDI-TOF-MS. The several proteins in the purified samples were identified as candidates of antimicrobial proteins.
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Report
(3 results)
Research Products
(9 results)
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[Journal Article] Oryzacystatin-II, a Cystatin from Rice(Oryza sativa L. japonica), is a Dimeric Protein: the Possible Involvement of the Interconversion between Dimer and Monomer in the Regulation of the Activity of Oryzacystatin-II2007
Author(s)
S., Ohtsubo, M., Taiyoji, T., Kawase, M., Taniguchi, E., Saitoh
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Journal Title
J.Agric.Food Chem Vol.55, No.5
Pages: 1762-1766
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Inhibition of the growth and proteolytic activity of Porphyromonas gingivalis by recombinant oryzacystatins2007
Author(s)
Yasuyuki, Shitomi, Yuki, Niwa, Tomokazu, Kawase, Sadami, Ohtsubo, Eiichi, Saitoh, Masayuki, Taniguchi
Organizer
10th Symposium on Proteinase Inhibitors and Biological Control
Place of Presentation
Portoroz, Slovenia
Description
「研究成果報告書概要(欧文)」より
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[Presentation] Screening of antimicrobial proteins from rice against Porphyromonas gingivalis and their properties2007
Author(s)
Yuki, Niwa, Akira, Ikarashi, Yasuyuki, Shitomi, Tomokazu, Kawase, Takaaki, Tanaka, Masayuki, Taniguchi
Organizer
Annual Meeting of the Society for Biotechnoly, Japan
Place of Presentation
Higasihiroshima(Hiroshma University)
Description
「研究成果報告書概要(欧文)」より
Related Report
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